Essential Role of CCL2 in Clustering of Splenic ERTR-9<sup>+</sup>Macrophages during Infection of BALB/c Mice by<i>Listeria monocytogenes</i>

Jabłońska, Jadwiga; Dittmar, Kurt E.J.; Kleinke, Tanja; Buer, Jan; Weiß, Siegfried

doi:10.1128/iai.00443-06

Cited by 17 publications

(24 citation statements)

References 38 publications

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“…This concept is supported by histological studies that describe a shift in the localization of Listeria in the spleen: initially trapped in the MZ, Listeria was found as early as 24 h after intravenous infection in the T cell zones [62]. Even though it cannot be formally excluded that T cells might primarily migrate to Listeria-containing foci in the MZ, as recently postulated by Weiss and colleagues [63], such a scenario seems -also with respect to the large body of published contrary findings in different infection models (see below) -far less likely.…”

Section: Splenic Cd8␣ + DC During Intracellular Bacterial Infectionmentioning

confidence: 71%

Unique functions of splenic CD8α+ dendritic cells during infection with intracellular pathogens

Neuenhahn

Busch

2007

Immunology Letters

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Section: Splenic Cd8␣ + DC During Intracellular Bacterial Infectionmentioning

confidence: 71%

Unique functions of splenic CD8α+ dendritic cells during infection with intracellular pathogens

Neuenhahn

Busch

2007

Immunology Letters

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“…More recently, CD8α + DC were found to be the primary cell type containing viable bacteria early after infection (1 – 3h) [28]. Other studies have detected LM exclusively in SIGN-R1 + MZMs and not CD11c + DC very early after infection after infection [14]. Here five phagocyte populations were identified that contained the bulk of LM after i.v.…”

Section: Introductionmentioning

confidence: 81%

“…These cells recognize sialic acid and LPS from Neiseria meningitidis [10] through Siglec-1 (sialic-acid-binding-immunoglobulin-like-lectin 1) [11, 12]. MMMs have also been shown to produce interferon during Herpes simplex virus infection [13], but their role in bacterial infection is unclear, although it was reported that they produce CCL2 [14]. …”

Section: Introductionmentioning

confidence: 99%

The cellular niche of Listeria monocytogenes infection changes rapidly in the spleen

et al. 2009

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The spleen is an important organ for the host response to systemic bacterial infections. Many cell types and cell surface receptors have been shown to play role in the capture and control of bacteria, yet these are often studied individually and a coherent picture has yet to emerge of how various phagocytes collaborate to control bacterial infection. We analyzed the cellular distribution of Listeria monocytogenes (LM) in situ during the early phase of infection. Using an immunohistochemistry approach, five distinct phagocyte populations contained LM after i.v. challenge and accounted for roughly all bacterial signal in tissue sections. Our analysis showed that LM was initially captured by a wide range of phagocytes in the marginal zone, where the growth of LM appeared to be controlled. The cellular distribution of LM within phagocyte populations changed rapidly during the first few hours, decreasing in marginal zone macrophages and transiently increasing in CD11c 1 DC. After 4-6 h LM was transported to the periarteriolar lymphoid sheath where the infective foci developed and LM grew exponentially.Key words: Bacterial infection . DC . Listeria monocytogenes . Macrophages . Spleen Supporting Information available online IntroductionThe spleen is an important site for host responses to bacterial infection [1,2]. Within the spleen, bacteria may encounter various tissue resident phagocytes including macrophages, DC, and neutrophils [3,4]. Marginal zone macrophages (MZM) are positioned along the outer layer of the marginal sinus where they have direct access to bacteria entering the spleen from the circulation. These macrophages express MARCO (macrophage receptor with collagenous structure), a type-1 scavenger receptor, related to the SR-A family of receptors [5], which recognizes bacterial cell-wall-associated polyanions [6]. MZM also express the C-type lectin, SIGN-R1. SIGN-R1 binds dextran [7] and facilitates the capture of polysaccharide antigens on bacteria such as Streptococcus pneumoniae [8]. A separate class of MZM, the metallophilic MZM (MMM), localize between the inner marginal sinus and the B-cell follicle [9]. These cells recognize sialic acid and LPS from Neiseria meningitidis [10] through Siglec-1 (sialicacid-binding-Ig-like-lectin 1) [11,12]. MMM have also been shown to produce interferon during Herpes simplex virus infection [13], but their role in bacterial infection is unclear, although it was reported that they produce CCL2 [14].Neutrophils play a crucial role in controlling bacterial infection [15][16][17][18][19]. They are present in the marginal zone (MZ) and Results LM enters a wide variety of phagocytes immediately after challengeWe found that the recovery of LM (EGD)-infected host cells from spleen was unreliable; $90% of LM CFU were lost in the process of isolating phagocytes and making single cell suspensions (Supporting Information Fig. 1). As an alternative, we developed an antibody staining approach, by which we identified five distinct phagocyte populations in spleen cryosections ( Fig....

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“…Moreover, we found that depleted SiglecH-DTR Tg mice were highly susceptible to lethal LM-OVA infection. Previous studies have shown that early after infection, Listeria can be found associated with SIGN-R1 + cells in the spleen (36, 37) and that mice depleted of MZM and MM are impaired in their ability to control and survive Listeria infection (38). We envision that lack of MZM in DT-treated SiglecH-DTR Tg mice may account for the cytokine storm and rapid death following LM-OVA infection.…”

Section: Discussionmentioning

confidence: 99%

Cell Depletion in Mice That Express Diphtheria Toxin Receptor under the Control of SiglecH Encompasses More Than Plasmacytoid Dendritic Cells

Swiecki

Wang

Riboldi

et al. 2014

The Journal of Immunology

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Plasmacytoid dendritic cells (pDC) produce type I interferon (IFN-I) in response to viruses and are routinely identified in mice by SiglecH expression. SiglecH is a sialic acid-binding immunoglobulin-like lectin that has an immunomodulatory role during viral infections. Here, we evaluated the impact of SiglecH deficiency on cytokine responses in the presence and absence of pDC. We found that lack of SiglecH enhanced IFN-I responses to viral infection regardless of whether pDC were depleted or not. We also examined the expression pattern of SiglecH in this study. We observed that SiglecH was expressed by specialized macrophages and progenitors of classical DC (cDC) and pDC. Accordingly, marginal zone macrophages and pDC precursors were eliminated in newly generated SiglecH-DTR Tg mice but not CLEC4C-DTR Tg mice after diphtheria toxin (DT) treatment. Using two different bacterial models, we found that SiglecH-DTR Tg mice injected with DT had altered bacterial uptake and were more susceptible to lethal Listeria monocytogenes infection than DT-treated CLEC4C-DTR Tg mice. Taken together, our findings suggest that lack of SiglecH may affect cytokine responses by cell types other than pDC during viral infections perhaps by altering viral distribution or burden and that cell depletion in SiglecH-DTR Tg mice encompasses more than pDC.

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Essential Role of CCL2 in Clustering of Splenic ERTR-9⁺Macrophages during Infection of BALB/c Mice byListeria monocytogenes

Cited by 17 publications

References 38 publications

Unique functions of splenic CD8α+ dendritic cells during infection with intracellular pathogens

Unique functions of splenic CD8α+ dendritic cells during infection with intracellular pathogens

The cellular niche of Listeria monocytogenes infection changes rapidly in the spleen

Cell Depletion in Mice That Express Diphtheria Toxin Receptor under the Control of SiglecH Encompasses More Than Plasmacytoid Dendritic Cells

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Essential Role of CCL2 in Clustering of Splenic ERTR-9+Macrophages during Infection of BALB/c Mice byListeria monocytogenes

Cited by 17 publications

References 38 publications

Unique functions of splenic CD8α+ dendritic cells during infection with intracellular pathogens

Unique functions of splenic CD8α+ dendritic cells during infection with intracellular pathogens

The cellular niche of Listeria monocytogenes infection changes rapidly in the spleen

Cell Depletion in Mice That Express Diphtheria Toxin Receptor under the Control of SiglecH Encompasses More Than Plasmacytoid Dendritic Cells

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Essential Role of CCL2 in Clustering of Splenic ERTR-9⁺Macrophages during Infection of BALB/c Mice byListeria monocytogenes