Essential rosemary oil protects testicular cells against DNA-damaging effects of H2O2 and DMNQ

Slameñová, D; Horváthová, Eva; Kovačíková, Zuzana; Kozics, Katarína; Hunáková, Ľuba

doi:10.1016/j.foodchem.2011.04.020

Cited by 15 publications

(13 citation statements)

References 27 publications

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“…Although EOs were applied in the past to successfully treat a variety of diseases and to preserve health, they have been used more frequently for a greater variety of applications in recent years, including drugs, crop protectants, food additives, aromatherapy, and others. The resulting increase in human exposure as a consequence of this expanded usage therefore requires a careful re-assessment of their toxicity and genotoxicity on the level of mammalian cells 27 . The potential toxic effects of plant extracts, including EOs, on humans should not be underestimated.…”

Section: Introductionmentioning

confidence: 99%

The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells

Puškárová

Bučková

Kraková

et al. 2017

Sci Rep

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183

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Six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. Antimicrobial activity was shown against pathogenic (Escherichia coli, Salmonella typhimurium, Yersinia enterocolitica, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis) and environmental bacteria (Bacillus cereus, Arthrobacter protophormiae, Pseudomonas fragi) and fungi (Chaetomium globosum, Penicillium chrysogenum, Cladosporium cladosporoides, Alternaria alternata, and Aspergillus fumigatus). Oregano, thyme, clove and arborvitae showed very strong antibacterial activity against all tested strains at both full strength and reduced concentrations. These essential oils showed different fungistatic and fungicidal activities when tested by direct application and in the vapor phase. The genotoxic effects of these oils on HEL 12469 human embryo lung cells were evaluated using an alkaline comet assay for the first time, revealing that none of the oils induced significant DNA damage in vitro after 24 h. This study provides novel approaches for assessing the antimicrobial potential of essential oils in both direct contact and the vapor phase and also demonstrates the valuable properties of the phenol-free arborvitae oil. These results suggest that all the tested essential oils might be used as broad-spectrum anti-microbial agents for decontaminating an indoor environment.

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Section: Introductionmentioning

confidence: 99%

The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells

Puškárová

Bučková

Kraková

et al. 2017

Sci Rep

Self Cite

183

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“…Consequently, in our previous experiments we evaluated the DNA-protective potential of CA against damage induced in HepG2 cells by hydrogen peroxide (H 2 O 2 ) and tert -butyl hydroperoxide, using conventional and modified comet assay (Slamenova et al , 2013b ). In our ex vivo studies we manifested the ability of CA and RO to protect DNA of hepatocytes and testicular cells isolated from CA- or RO-supplemented rats against several DNA-damaging agents (Slamenova et al , 2008 ; Horvathova et al , 2010 ; Slamenova et al , 2011a ; Slamenova et al , 2011b ). We examined also the anticancer potential of these two volatiles on human hepatoma cell line HepG2 (Melusova et al , 2014a ) and on BHNF-1 fibroblasts (Melusova et al , 2014b ).…”

Section: Discussionmentioning

confidence: 97%

Carvacrol and rosemary oil at higher concentrations induce apoptosis in human hepatoma HepG2 cells

Melušová

Jantová

Horváthová

2014

Interdisciplinary Toxicology

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Natural essential oils are volatile herbal complex compounds which manifest cytotoxic effects on living cells depending on their type and concentration but usually they are not genotoxic. Our previous studies showed that carvacrol (CA) and rosemary essential oil (RO) induced growth inhibition of both human cell lines HepG2 and BHNF-1, with hepatoma HepG2 cells being more sensitive to either compound tested. Cytotoxic concentrations of CA and RO induced the formation of DNA strand breaks. Further ex vivo studies showed that extracts prepared from hepatocytes of CA- and RO-supplemented rats did not increase incision repair activity compared to extracts from liver cells of control animals. Therefore, the aim of this work was to determine the effect of cytotoxic concentrations of CA and RO on the cell cycle and the ability of both natural volatiles to induce DNA fragmentation and apoptotic death of human hepatoma HepG2 cells. These effects were measured after 24 h incubation of HepG2 cells with CA and RO using three independent methods – flow cytometry, internucleosomal DNA fragmentation (electrophoresis) and micronucleus assay. Evaluation of morphological changes and formation of micronuclei in HepG2 cells showed no increase in the number of micronuclei in cells treated by CA and RO compared to control cells. On the other hand, CA and RO induced morphological changes typical for apoptosis in concentration-dependent manner. The presence of necrosis was negligible. Both natural compounds caused shrinking of cytoplasmic membrane and formation of apoptotic bodies. In addition, the highest concentrations of CA and RO induced internucleosomal DNA fragmentation (formation of DNA ladder) in HepG2 cells. Cell cycle analysis revealed the accumulation of cells in the G1 phase, which was accompanied by a reduction in the number of cells in the S phase after 24 h exposure to the substances tested. The cell division was thus slowed down or stopped and this process resulted in cell death.

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“…Already in 2002 it was found that long-term or short-term pre-treatment of hamster V79 or human colon cancer Caco-2 cells with extracts of RO manifested protective effects against DNA damage induced by H 2 O 2 and visible light-excited methylene blue [59]. The potential DNA-protective effects of RO oil (dissolved in drinking water of rats) were demonstrated in hepatocytes influenced with H 2 O 2 , visible light-excited methylene blue and DMNQ [18] and in testicular cells treated with H 2 O 2 and DMNQ [60]. Melusova et al [31] studied DNA-protective and pro-apoptotic effect of carvacrol and RO essential oil as well as repair ability of extracts acquired from hepatocytes of rats fed with carvacrol or RO oil.…”

Section: Discussionmentioning

confidence: 98%

“…The authors suggested that although RO supplementation improved the status of oxidative stress of old rats, the antioxidant status of their organs was not improved. Slamenova et al [60] showed that consumption of RO oil, which increased resistance of rat testicular cells against DNA-damaging effects of H 2 O 2 and DMNQ, did not change the activity of antioxidant enzymes GPx and SOD.…”

Section: Discussionmentioning

confidence: 99%

Chromatographic analyses of Lavandula angustifolia and Rosmarinus officinalis extracts and their biological effects in mammalian cells and cell-free systems

Horváthová¹,

Kozics²,

Melušová³

et al. 2017

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Knowledge of biological properties of natural compounds allows to understand their therapeutic value, efficacy and security. We investigated: composition of Lavandula angustifolia (LA) and Rosmarinus officinalis (RO) extracts, their antioxidant capacity, cytotoxicity and genotoxicity, their DNA-protective potential against DNA damage induced in hamster V79 cells by several genotoxins or in plasmid DNA by Fe2+ ions and activity of antioxidant enzymes in cells treated with these extracts. Higher cytotoxicity, observed at higher concentrations of extracts, was accompanied by the increased level of single-strand (ss) DNA breaks as well as formamidopyrimidine DNA glycosylase (Fpg) sensitive sites. LA and RO extracts were able to protect DNA of hamster cells as well as plasmid DNA against ss DNA breaks induced by genotoxins and Fe2+. LA extract mildly increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), while RO extract decreased the activity of SOD, but increased the activity of CAT and GPx. Cell-free tests confirmed antioxidant activity of both extracts. The biological properties of LA and RO extracts showed that they could have a positive impact on human health.

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Essential rosemary oil protects testicular cells against DNA-damaging effects of H2O2 and DMNQ

Cited by 15 publications

References 27 publications

The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells

The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells

Carvacrol and rosemary oil at higher concentrations induce apoptosis in human hepatoma HepG2 cells

Chromatographic analyses of Lavandula angustifolia and Rosmarinus officinalis extracts and their biological effects in mammalian cells and cell-free systems

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