EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin

Kondo, Hidehiro; Suga, Ryota; Suda, Satomitsu; Nozaki, Reiko; Hirono, Ikuo; Nagasaka, Reiko; Kaneko, Gen; Ushio, Hideki; Watabe, Shugo

doi:10.1016/j.gene.2011.05.035

Cited by 32 publications

(24 citation statements)

References 24 publications

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“…As is the case in zebrafish (Nishio et al, 2008), and the very recent study performed in rainbow trout (Kondo et al, 2011), we have also observed that the expression of adiponectin in muscles is higher than in adipose tissue. In addition, adiponectin mRNA expression was two times higher in the oxidative red muscle than in the glycolytic white muscle, which corresponds with previous studies assessing different types of fibres in mice (Krause et al, 2008).…”

Section: Discussionsupporting

confidence: 83%

“…Nevertheless, there is little information available about adipokines in lower vertebrates such as fish. Although the presence of leptin and tumour necrosis factor  (TNF) has been described in various fish species (Kling et al, 2009;SaeraVila et al, 2007), adiponectin has been identified in zebra fish (Nishio et al, 2008), and only very recently in rainbow trout (Kondo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

Sanchez-Gurmaches

Cruz-García

Gutiérrez

et al. 2012

Journal of Experimental Biology

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SUMMARYHere we present the presence of adiponectin and adiponectin receptors [type 1 (adipoR1) and type 2 (adipoR2)] in rainbow trout (Oncorhynchus mykiss) tissues and cell cultures together with the response to different scenarios. In response to fasting, adiponectin expression was up-regulated in adipose tissue, while the expression of its receptors increased in white and red muscle. Insulin injection decreased adipoR1 expression in white and red muscles. We deduce that the adipoRs in trout muscle show opposite responses to increasing insulin plasma levels, which may maintain sensitivity to insulin in this tissue. Adiponectin expression was inhibited by the inflammatory effect of lipopolysaccharide (LPS) in adipose tissue and red muscle. Moreover, results indicate that LPS may lead to mobilization of fat reserves, increasing adipoR1 expression in adipose tissue. The effects of LPS could be mediated through tumour necrosis factor  (TNF), at least in red muscle. Insulin, growth hormone and TNF all diminished expression of adipoR2 in adipocytes and adipoR1 in myotubes, while insulin increased the expression of adipoR2 in the muscle cells. Adiponectin activates Akt in rainbow trout myotubes, which may lead to an increase in fatty acid uptake and oxidation. Overall, our results show that the adiponectin system responds differently to various physiological challenges and that it is hormonally controlled in vivo and in vitro. To the best of our knowledge, this is the first time this has been demonstrated in teleosts, and it may be a valuable contribution to our understanding of adipokines in fish.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

Sanchez-Gurmaches

Cruz-García

Gutiérrez

et al. 2012

Journal of Experimental Biology

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“…In this sense, FABP4 acts as an adipokine regulating adipocyte and macrophage interactions during inflammation [106]. FABP3 transcripts have been predominantly detected in subcutaneous adipocytes [107] and intra-peritoneal adipose tissue of rainbow trout [23]. The high presence of FABPs suggests an important role for this family of proteins during the maturation process of fish adipocytes.…”

Section: Resultsmentioning

confidence: 99%

“…For instance, the cytokine tumor necrosis factor α (TNFα) is expressed in fish adipose tissue and is well known by its anti-adiposity actions [5, 17, 18]. Leptin and adiponectin are known to be the most abundant proteins secreted by mammalian adipocytes [19], contrary to what has been described so far in teleost species, where leptin is primarily expressed in the liver [20] and adiponectin in muscle [21–23]. These specific features highlight the need to further investigate the physiological and secretory functions of fish adipose tissue.…”

Section: Introductionmentioning

confidence: 99%

Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells

et al. 2017

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BackgroundExcessive accumulation of adipose tissue in cultured fish is an outstanding problem in aquaculture. To understand the development of adiposity, it is crucial to identify the genes which expression is associated with adipogenic differentiation. Therefore, the transcriptomic profile at different time points (days 3, 8, 15 and 21) along primary culture development of rainbow trout preadipocytes has been investigated using an Agilent trout oligo microarray.ResultsOur analysis identified 4026 genes differentially expressed (fold-change >3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation. Proliferation cluster comprised 1028 genes up-regulated from days 3 to 8 of culture meanwhile the differentiation cluster was characterized by 2140 induced genes from days 15 to 21. Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. In addition, the implication of the eicosanoid signalling pathway was highlighted during this phase. On the other hand, the terminal differentiation phase was enriched with genes involved in energy production, lipid and carbohydrate metabolism. Moreover, during this phase an enrichment in genes involved in the formation of the lipid droplets was evidenced as well as the activation of the thyroid-receptor/retinoic X receptor (TR/RXR) and the peroxisome proliferator activated receptors (PPARs) signalling pathways. The whole adipogenic process was driven by a coordinated activation of transcription factors and epigenetic modulators.ConclusionsOverall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. Furthermore, the information generated will allow future investigations of specific genes involved in particular stages of fish adipogenesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3728-0) contains supplementary material, which is available to authorized users.

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“…Adiponectin and adiponectin receptors (adipoR1 and adipoR2) have been identified in zebra fish and rainbow trout (O. mykiss) (Kondo et al, 2011;Nishio et al, 2008;Sánchez-Gurmaches et al, 2012a). Adiponectin activates Akt in rainbow trout myotubes, promotes the uptake and oxidation of fatty acid, and further modulates insulin sensitivity, glucose homeostasis and lipid metabolism (Fig.…”

Section: The Effect Of Tnfα On Adiponectinmentioning

confidence: 99%

Tumor necrosis factor alpha is a potent regulator in fish adipose tissue

Liu¹,

Mai²,

Ai³

2015

Aquaculture

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EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin

Cited by 32 publications

References 24 publications

Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells

Tumor necrosis factor alpha is a potent regulator in fish adipose tissue

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