EST-SSR development and genetic diversity in the medicinal plant Pseudostellaria heterophylla (Miq.) Pax

Xu, Liyi; Li, PINGPING; Su, JINGJING; Wang, DING; Kuang, Yun-Bo; Ye, ZUYUN; Chen, MEIXIA

doi:10.1016/j.jarmap.2022.100450

Cited by 7 publications

(9 citation statements)

References 26 publications

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“…However, phenotypes are easily affected by cultivation methods and environmental factors and require long-term observation ( Chen et al., 2010 ). In addition, the irregular introduction of P. heterophylla has also impacted the distribution of P. heterophylla genetic resources, which affected the uniform collection, classification, and identification of germplasm resources ( Xu et al., 2023 ). P. heterophylla cultivation has a history of more than 180 years, and at its earliest stage of cultivation, P. heterophylla germplasm resources were mainly derived from wild populations.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, the chloroplast genome rbcL and matK regions, the Internal Transcribed Spacers (ITS) of the nuclear ribosomal DNA, sequence-related amplified polymorphism (SRAP), inter simple sequence repeat (ISSR), and expressed sequence tagsimple sequence repeat (ESR-SSR) have been used to characterize the genetic diversity of P. heterophylla germplasm (Yi et al, 2013;Xiao et al, 2014;Xu et al, 2023). Yi et al (2013) found that the ITS sequences of different P. heterophylla varieties had several specific single nucleotide mutation sites and could be used to identify and distinguish samples from nine different producing areas.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of 17 complete chloroplast genomes reveals intraspecific variation and relationships among Pseudostellaria heterophylla (Miq.) Pax populations

et al. 2023

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Pseudostellaria heterophylla (Miq.) Pax is a well-known medicinal and ecologically important plant. Effectively distinguishing its different genetic resources is essential for its breeding. Plant chloroplast genomes can provide much more information than traditional molecular markers and provide higher-resolution genetic analyses to distinguish closely related planting materials. Here, seventeen P. heterophylla samples from Anhui, Fujian, Guizhou, Hebei, Hunan, Jiangsu, and Shandong provinces were collected, and a genome skimming strategy was employed to obtain their chloroplast genomes. The P. heterophylla chloroplast genomes ranged from 149,356 bp to 149,592 bp in length, and a total of 111 unique genes were annotated, including 77 protein-coding genes, 30 tRNA genes, and four rRNA genes. Codon usage analysis showed that leucine had the highest frequency, while UUU (encoding phenylalanine) and UGC (encoding cysteine) were identified as the most and least frequently used codons, respectively. A total of 75–84 SSRs, 16–21 short tandem repeats, and 27–32 long repeat structures were identified in these chloroplast genomes. Then, four primer pairs were revealed for identifying SSR polymorphisms. Palindromes are the dominant type, accounting for an average of 47.86% of all long repeat sequences. Gene orders were highly collinear, and IR regions were highly conserved. Genome alignment indicated that there were four intergenic regions (psaI-ycf4, ycf3-trnS, ndhC-trnV, and ndhI-ndhG) and three coding genes (ndhJ, ycf1, and rpl20) that were highly variable among different P. heterophylla samples. Moreover, 10 SNP/MNP sites with high polymorphism were selected for further study. Phylogenetic analysis showed that populations of Chinese were clustered into a monophyletic group, in which the non-flowering variety formed a separate subclade with high statistical support. In this study, the comparative analysis of complete chloroplast genomes revealed intraspecific variations in P. heterophylla and further supported the idea that chloroplast genomes could elucidate relatedness among closely related cultivation materials.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of 17 complete chloroplast genomes reveals intraspecific variation and relationships among Pseudostellaria heterophylla (Miq.) Pax populations

et al. 2023

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“…Pseudostellaria heterophylla (Taizishen in Chinese), a medicinal, edible, and ornamental plant of Caryophyllaceae that is rich in polysaccharides, flavonoids, saponins, minerals, and amino acids, has great medicinal, nutritional, and economic value [1][2][3][4]. Its dried tuberous root has many beneficial and pharmacological functions; it is able to inhibit tumor cells, moisten the lungs, protect the myocardium, invigorate the spleen and stomach, benefit the blood, and enhance immunity [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of Oligochitosan and Pyraclostrobin in Controlling Leaf Spot Disease in Pseudostellaria heterophylla

Zhang,

Tang,

Wang

et al. 2024

Antibiotics

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Pseudostellaria heterophylla (or Taizishen in Chinese), a medicinal, edible, and ornamental Chinese herb, is seriously affected by leaf spot disease (LSD). Oligochitosan is a natural agricultural antibiotic that is produced via the degradation of chitosan, which is deacetylated from chitin; pyraclostrobin is a broad-spectrum and efficient strobilurin fungicide. In this work, the ability of pyraclostrobin, oligochitosan, and their formula to manage P. heterophylla leaf spot disease and their role in its resistance, leaf photosynthesis, agronomic plant traits, root growth, and root quality were studied. The results show that the joint application of oligochitosan and low-dosage pyraclostrobin could control LSD more efficiently, with control effects of 85.75–87.49% compared to high-dosage pyraclostrobin or oligochitosan alone. Concurrently, the application of this formula could more effectively improve the resistance, leaf photosynthesis, agronomic plant traits, root yield, and medicinal quality of P. heterophylla, as well as reduce the application of pyraclostrobin. This finding suggests that 30% pyraclostrobin suspension concentrate (SC) 1500-time + 5% oligosaccharin aqueous solutions (AS) 500-time diluent can be recommended for use as a feasible formula to manage LSD and reduce the application of chemical pesticides.

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“…Pseudostellaria heterophylla (Miq.) Pax, a popular clinical herb and nutritious health food, is widely distributed in China, Japan, the Russian Far East, South Korea, and North Korea [ 1 ]. Its dried tuberous root is rich in polysaccharides, saponins, cyclic peptides, flavonoids, amino acids, and minerals, and has high medicinal, nutritional, and commercial values [ 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan Enhances Low-Dosage Difenoconazole to Efficiently Control Leaf Spot Disease in Pseudostellaria heterophylla (Miq.) Pax

Zhang,

Dai,

Liu

et al. 2023

Molecules

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Pseudostellaria heterophylla (Miq.) Pax is a popular clinical herb and nutritious health food. However, leaf spot disease caused by fungal pathogens frequently occurs and seriously influences the growth, quality, and yield of P. heterophylla. In this work, the field control roles of difenoconazole, chitosan, and their combination in the leaf spot disease in P. heterophylla and their effects on the disease resistance, photosynthetic capacity, medicinal quality, and root yield of P. heterophylla are investigated. The results manifest that 37% difenoconazole water-dispersible granule (WDG) with 5000-time + chitosan 500-time dilution liquid had a superior control capacity on leaf spot disease with the control effects of 91.17%~88.19% at 15~30 days after the last spraying, which significantly (p < 0.05) exceeded that of 37% difenoconazole WDG 3000-time dilution liquid and was significantly (p < 0.01) higher than that of 37% difenoconazole WDG 5000-time dilution liquid, chitosan 500-time dilution liquid, or chitosan 1000-time dilution liquid. Simultaneously, this combination could more effectively enhance the disease resistance, photosynthetic capacity, medicinal quality, and tuberous root yield of P. heterophylla compared to when these elements were applied alone, as well as effectively reduce difenoconazole application. This study emphasizes that chitosan combined with a low dosage of difenoconazole can be proposed as a green, efficient, and alternative formula for controlling leaf spot disease in P. heterophylla and enhancing its resistance, photosynthesis, quality, and yield.

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EST-SSR development and genetic diversity in the medicinal plant Pseudostellaria heterophylla (Miq.) Pax

Cited by 7 publications

References 26 publications

Comparative analysis of 17 complete chloroplast genomes reveals intraspecific variation and relationships among Pseudostellaria heterophylla (Miq.) Pax populations

Comparative analysis of 17 complete chloroplast genomes reveals intraspecific variation and relationships among Pseudostellaria heterophylla (Miq.) Pax populations

Synergistic Effects of Oligochitosan and Pyraclostrobin in Controlling Leaf Spot Disease in Pseudostellaria heterophylla

Chitosan Enhances Low-Dosage Difenoconazole to Efficiently Control Leaf Spot Disease in Pseudostellaria heterophylla (Miq.) Pax

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