Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique

Abstract

Using minimally invasive methods to model spinal cord injury (SCI) can minimize behavioral and histological differences between experimental animals, thereby improving the reproducibility of the experiments.These methods need two requirements to be fulfilled: clarity of the surgical anatomical pathway and simplicity and convenience of the laboratory device. Crucially for the operator, a clear anatomical pathway provides minimally invasive exposure, which avoids additional damage to the experimental animal during the surgical procedures and allows the animal to maintain a consistent and stable anatomical morphology during the experiment.In this study, the use of a novel integrated platform called the SCI coaxial platform for spinal cord injury in small animals to expose the T9 level spinal cord in a minimally invasive way and stabilize and immobilize the vertebra of mice using a vertebral stabilizer is researched, and, finally, a coaxial gravity impactor is used to contuse the spinal cord of mice to approach different degrees of T9 spinal cord injury. Finally, histological results are provided as a reference for the readers.

A novel minimally invasive and versatile kyphoplasty balloon-based model of porcine spinal cord injury

“…Previously described minimally invasive animal models of spinal cord injury include models that minimize the amount of bone removed during the laminectomy portion of the injury surgery ( 15 , 16 ), models involving microscopic dissection and/or operating techniques intended to minimize collateral tissue trauma ( 17 ), and models wherein a balloon compression device is inserted into the epidural or subdural space via a small hemilaminectomy defect ( 18 – 20 ). While each of these are less invasive than a typical open surgical laminectomy, they each still involve muscle dissection and bony removal, and are subject to the limitations of laminectomy in a spinal cord injury model discussed previously.…”

“…A variety of minimally invasive animal models for spinal cord injury have been developed, including models wherein a small laminectomy or hemilaminectomy is utilized to minimize bony removal ( 15 , 16 ), and models involving microscopic dissection and/or operating techniques intended to minimize collateral tissue trauma ( 17 ). Each of these, however, still requires muscle dissection and removal of bone over the site of injury.…”

Exploration of the effect and potential mechanism of quercetin in repairing spinal cord injury based on network pharmacology and in vivo experimental verification

“…For each in vivo experiment, rats were randomly divided into 3 groups of 6 rats. The SCI model was performed as per previously published reports [ 19 ], on Feng's Standard SCI coaxial platform [ 20 ]. Briefly, rats were anesthetized with isoflurane and the spinal cord was exposed after T10 laminectomy.…”

CREB1 Facilitates GABAergic Neural Differentiation of Human Mesenchymal Stem Cells through BRN2 for Pain Alleviation and Locomotion Recovery after Spinal Cord Injury

“…On the day before modeling, all mice were administered cyclosporine A (HY-B0579, MCE, Middlesex, NJ, USA) via intraperitoneal injection at a dosage of 10 mg/kg/day. The contusion SCI model was established on the next day following previously published protocols [23,24]. After SCI, the treatment was applied to the mice in different groups.…”