Establishing a process of irradiating small animal brain using a CyberKnife and a microCT scanner

Kim, Hak‐Soo; Fabien, Jeffrey; Zheng, Yiran; Yuan, Jake; Brindle, James; Sloan, Andrew E.; Yao, Min; Wessels, Barry W.; Machtay, Mitchell; Welford, Scott M.; Sohn, Jason W.

doi:10.1118/1.4861713

Cited by 11 publications

(16 citation statements)

References 28 publications

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“…Cradles have been routinely used to facilitate the accuracy of cranial irradiation in small animals (11)(12)(13)(14) or co-registration of multimodality and longitudinal images (15)(16)(17)(18). Among various cradles for the mouse head and neck region, the tooth bar anchoring upper incisors is essential.…”

Section: Discussionmentioning

confidence: 99%

Automated MicroSPECT/MicroCT Image Analysis of the Mouse Thyroid Gland

Cheng

Hollingsworth

Scarberry

et al. 2017

Thyroid

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Section: Discussionmentioning

confidence: 99%

Automated MicroSPECT/MicroCT Image Analysis of the Mouse Thyroid Gland

Cheng

Hollingsworth

Scarberry

et al. 2017

Thyroid

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“…The 3D printing technologies have since been utilized in radiotherapy. Kim et al (18) 3D‐printed a mouse stereotactic body mold using SLA technology for CyberKnife QA studies. Nie et al (19) 3D‐printed 192 Ir‐based small animal apparatus.…”

Section: Introductionmentioning

confidence: 99%

Potential of 3D printing technologies for fabrication of electron bolus and proton compensators

Zou

Fisher²,

Zhang

et al. 2015

J Applied Clin Med Phys

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In electron and proton radiotherapy, applications of patient‐specific electron bolus or proton compensators during radiation treatments are often necessary to accommodate patient body surface irregularities, tissue inhomogeneity, and variations in PTV depths to achieve desired dose distributions. Emerging 3D printing technologies provide alternative fabrication methods for these bolus and compensators. This study investigated the potential of utilizing 3D printing technologies for the fabrication of the electron bolus and proton compensators. Two printing technologies, fused deposition modeling (FDM) and selective laser sintering (SLS), and two printing materials, PLA and polyamide, were investigated. Samples were printed and characterized with CT scan and under electron and proton beams. In addition, a software package was developed to convert electron bolus and proton compensator designs to printable Standard Tessellation Language file format. A phantom scalp electron bolus was printed with FDM technology with PLA material. The HU of the printed electron bolus was 106.5±15.2. A prostate patient proton compensator was printed with SLS technology and polyamide material with −70.1±8.1 HU. The profiles of the electron bolus and proton compensator were compared with the original designs. The average over all the CT slices of the largest Euclidean distance between the design and the fabricated bolus on each CT slice was found to be 0.84±0.45 mm and for the compensator to be 0.40±0.42 mm. It is recommended that the properties of specific 3D printed objects are understood before being applied to radiotherapy treatments.PACS number: 81.40

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“…An Institutional Animal Care and Use Committee protocol (protocol #2013-0063) was approved prior to initiating animal experiments. We previously developed a custom stereotactic immobilization device with an accuracy of 2 mm 14 and now demonstrate an improved device with an accuracy of 1 mm. The new device immobilizes the mouse with a spring-loaded bite block as the mouse is stretched to full extension in prone position ( Figure 1).…”

Section: Developing a Stereotactic Base Plate And Body Mold Systemmentioning

confidence: 99%

“…We have reported on the use of CyberKnife for the delivery of conformal RT in mice. 14 A 3-dimensional (3D)-printed stereotactic body mold was used, and 3 fiducial marks were placed on the mold. The positional accuracy between mice was 1.41 ± 0.73 mm, which was added to the target volume to generate a planning target volume (PTV).…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Small Animal Immobilizer and Positioning System for the Study of Delivery of Intracranial and Extracranial Radiotherapy Using the Gamma Knife System

Awan

Dorth

Mani

et al. 2016

Technol Cancer Res Treat

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The purpose of this research is to establish a process of irradiating mice using the Gamma Knife as a versatile system for small animal irradiation and to validate accurate intracranial and extracranial dose delivery using this system. A stereotactic immobilization device was developed for small animals for the Gamma Knife head frame allowing for isocentric dose delivery. Intercranial positional reproducibility of a reference point from a primary reference animal was verified on an additional mouse. Extracranial positional reproducibility of the mouse aorta was verified using 3 mice. Accurate dose delivery was validated using film and thermoluminescent dosimeter measurements with a solid water phantom. Gamma Knife plans were developed to irradiate intracranial and extracranial targets. Mice were irradiated validating successful targeted radiation dose delivery. Intramouse positional variability of the right mandible reference point across 10 micro-computed tomography scans was 0.65 ± 0.48 mm. Intermouse positional reproducibility across 2 mice at the same reference point was 0.76 ± 0.46 mm. The accuracy of dose delivery was 0.67 ± 0.29 mm and 1.01 ± 0.43 mm in the coronal and sagittal planes, respectively. The planned dose delivered to a mouse phantom was 2 Gy at the 50% isodose with a measured thermoluminescent dosimeter dose of 2.9 ± 0.3 Gy. The phosphorylated form of member X of histone family H2A (γH2AX) staining of irradiated mouse brain and mouse aorta demonstrated adjacent tissue sparing. In conclusion, our system for preclinical studies of small animal irradiation using the Gamma Knife is able to accurately deliver intracranial and extracranial targeted focal radiation allowing for preclinical experiments studying focal radiation.

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Establishing a process of irradiating small animal brain using a CyberKnife and a microCT scanner

Abstract: With the use of a stereotactic body mold with fiducial markers, microCT imaging, and resolution down-sampling, the CyberKnife system can successfully perform small-animal radiotherapy studies.

Cited by 11 publications

References 28 publications

Automated MicroSPECT/MicroCT Image Analysis of the Mouse Thyroid Gland

Automated MicroSPECT/MicroCT Image Analysis of the Mouse Thyroid Gland

Potential of 3D printing technologies for fabrication of electron bolus and proton compensators

Development and Validation of a Small Animal Immobilizer and Positioning System for the Study of Delivery of Intracranial and Extracranial Radiotherapy Using the Gamma Knife System

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