Establishing a straightforward I‐SceI‐mediated recombination one‐plasmid system for efficient genome editing in P. putidaKT2440
Hao Meng,
Sebastian Köbbing,
Lars M. Blank
Abstract:Pseudomonas putida has become an increasingly important chassis for producing valuable bioproducts. This development is not least due to the ever‐improving genetic toolbox, including gene and genome editing techniques. Here, we present a novel, one‐plasmid design of a critical genetic tool, the pEMG/pSW system, guaranteeing one engineering cycle to be finalized in 3 days. The pEMG/pSW system proved in the last decade to be valuable for targeted genome engineering in Pseudomonas, as it enables the deletion of l… Show more
Lignin is an aromatic biopolymer found in plant cell walls, that is the must abundant source of renewable aromatic carbon in the biosphere. Hence there is considerable interest in the...
Lignin is an aromatic biopolymer found in plant cell walls, that is the must abundant source of renewable aromatic carbon in the biosphere. Hence there is considerable interest in the...
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