Establishing Germline Chimeric Chickens Using Primordial Germ Cells

Furuta, Hiroyuki

doi:10.2141/jpsa.011053

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…Qian et al (2010) reported that PGCs was formed out of gonad and migrated toward gonad during embryogenesis which will be developed into spermatozoa and ovum. Primordial germ cells might be used as genetic source and for establishing transgenic chicken (Furuta 2012). Primordial germ cells were cells with plurypotent characteristic making the PGCs as a good model to study embryonic development by in-vitro (Wang et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Determination of production capacity of circulated primordial germ cells (circulated-PGCs) of KUB chicken using lysis buffer ammonium chloride potassium (ACK)

Sopiyana

Supriatna

Setiadi

et al. 2016

JITV

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In poultry embryos, primordial germ cells (PGCs) are progenitor cells for gametes, which have unique migration pathway. Primordial germ cells arise from epiblast in germinal crescent and circulate through the bloodstream for a short period of time, then leave blood vessel to migrate toward gonads. The aim of this study was to determine the potential production capacity of circulated-PGCs of KUB chicken at different developmental stages of embryo using a rapid and simple method. Seventy five KUB chicken fertile eggs were divided into five groups and incubated at 38.5 0C with a humidity of 60%. Hatching was set to the embryonic development stage of 14-18. The blood was collected through dorsal aorta using micropipette under microscope. The collected blood was placed in a 1.5 ml eppendorf tube which was previously filled with 100 µl phosphate buffered saline without Ca2+ and Mg2+ (PBS-) mixed with fetal bovine serum (FBS) with a ratio of 90%:10%. The PGCs were purified using lysis buffer ammonium chloride potassium method. The results showed that average production of circulated-PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P <0.05). The average production of circulated-PGCs at stage 14, 15, 16, 17, and 18 were 37.9; 53.5; 49.8; 38.3; and 33.5 respectively. The number of circulated-PGCs was not different among stages 14, 17 nor 18. The highest number of circulated-PGCs of KUB chicken was obtained at stage 15, so that the isolation and collection of PGCs through the blood circulation was recommended in stage 15.Key Words: KUB Chicken, PGCs, Embryonic Development Stage, Ammonium Chloride Potassium

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Section: Introductionmentioning

confidence: 99%

Determination of production capacity of circulated primordial germ cells (circulated-PGCs) of KUB chicken using lysis buffer ammonium chloride potassium (ACK)

Sopiyana

Supriatna

Setiadi

et al. 2016

JITV

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“…Pembentukan ayam germline chimera mempunyai arti penting untuk penelitian fisiologi, tingkah laku dan biologi perkembangan. Germline chimera mempunyai aplikasi yang sangat potensial untuk generasi unggas transgenik, untuk konservasi spesies yang terancam punah dan untuk melakukan perubahan seks rasio pada industri unggas pedaging (Ishiguro et al 2008;Kang et al 2008;Furuta 2012). Germline chimera mempunyai aplikasi praktis di dalam konservasi spesies dan stok genetik yang ada, karena dapat memelihara material genetik dengan cara menyimpan PGCs yang dibekukan dibandingkan dengan memelihara hewan hidup (Mozdziak et al 2006).…”

Section: Gambar 3 Morfologi Pgcs Setelah Kriopreservasiunclassified

Purification Method and Storage of Primordial Germ Cells-Circulation for Preservation of Local Chicken

Kostaman¹

2015

Indonesian Bulletin of Animal and Veterinary Sciences

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Primordial germ cells (PGCs) dapat dipergunakan untuk produksi ayam transgenik dan penyimpanan material genetik pada spesies unggas. Primordial germ cells merupakan sel-sel germinal yang terproliferasi, yang kemudian akan berubah bentuk menjadi spermatogonia di testis atau oogonia di ovarium. Primordial germ cells memiliki jalur migrasi yang unik, sehingga PGCs dapat dijaring dan dikoleksi dari darah embrio, kemudian diperbanyak serta dikembangkan dengan kultur. Makalah ini menguraikan tentang isolasi, koleksi, purifikasi, penyimpanan dan transfer PGCs-sirkulasi dari ayam-ayam lokal yang ada di Indonesia. Sejumlah PGCs-sirkulasi diisolasi dari ayam Gaok pada stadium 15 sebagai tingkat aman dengan krioprotektan DMSO dan setelah ditransfer ke embrio resipien menghasilkan anak-anak ayam. Dengan demikian, teknologi ini dapat dimanfaatkan untuk pelestarian plasma nutfah ayam lokal Indonesia.

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“…Numerous studies had reported the isolation and collection of PGCs from gonadal embryos (Nakamura et al, 2011;Nakajima et al, 2011;Nakajima et al, 2016). Moreover, germline chimeras had been produced in chicken by transferring PGCs or GGCs into the bloodstream at 2-day-old recipient embryos (Furuta et al, 2007(Furuta et al, , 2008 so that PGCs could be used as a genetic resource to produce germ line chimera (Furuta, 2012). Nakajima et al (2012) reported that gonadal PGCs collected from 7-day-old chick embryos and incubated in PBS [-] can be used to produce germline chimeras.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken

Sopiyana¹,

Setiadi

Fahrudin

et al. 2017

Med Pet

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Primordial germ cells (PGCs) are cells that will differentiate themselves into spermatogonia in the testis or oogonia in the ovary. Primordial germ cells arise from epiblast and circulate through the bloodstream and finally entering gonadal anlage. The aim of this study was to determine the number of gonadal PGCs of KUB chicken at different development stages. Sixty KUB chicken fertile eggs were divided into four groups (6, 7, 8, and 9 days incubation periods), and incubated at 38 o C with a humidity of 60%. Harvesting was synchronized to the embryonic development at 6-9 d. Gonads were collected using sharp tweezers, and were placed in Eppendorf tube 1.5 mL containing 500 µL PBS [-]. Gonadal PGCs were purified using PBS [-]. The results showed that the average number of gonadal PGCs at 6, 7, 8, and 9 d were 113.7; 143.5; 92.9; and 85.7 cells per embryo, respectively. Number of gonadal PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P<0.05), which reached a peak at day 7 of incubation, so that the isolation and collection of PGCs from the gonads were recommended at day 7 of incubation. This information is useful in production of germline chimera of other Indonesian local chickens. Keywords

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Establishing Germline Chimeric Chickens Using Primordial Germ Cells

Cited by 4 publications

References 48 publications

Determination of production capacity of circulated primordial germ cells (circulated-PGCs) of KUB chicken using lysis buffer ammonium chloride potassium (ACK)

Determination of production capacity of circulated primordial germ cells (circulated-PGCs) of KUB chicken using lysis buffer ammonium chloride potassium (ACK)

Purification Method and Storage of Primordial Germ Cells-Circulation for Preservation of Local Chicken

Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken

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