Establishing patient-derived organoids from human endometrial cancer and normal endometrium

Abstract

Endometrial cancer is the most common gynecologic malignancy in the United States and is one of the few malignancies that had an increasing incidence and mortality rate over the last 10 years. Current research models fail to recapitulate actual characteristics of the tumor that are necessary for the proper understanding and treatment of this heterogenous disease. Patient-derived organoids provide a durable and versatile culture system that can capture patient-specific characteristics such as the mutational profile and response to therapy of the primary tumor. Here we describe the methods for establishing, expansion and banking of endometrial cancer organoids to develop a living biobank. Samples of both endometrial tumor tissue and matched normal endometrium were collected from 10 patients. The tissue was digested into single cells and then cultured in optimized media to establish matched patient endometrial cancer and normal endometrial tissue organoids. Organoids were created from all major endometrial cancer histologic subtypes. These organoids are passaged long term, banked and can be utilized for downstream histological and genomic characterization as well as functional assays such as assessing the response to therapeutic drugs.

Bioengineering approaches for the endometrial research and application

“…Organoids are used as in vitro culture models for studying endometrial cancer [ 121 ]. Endometrial cancer organoids have successfully recapitulated tumor histology and morphology [ 122 ].…”

Organoids, Biocybersecurity, and Cyberbiosecurity—A Light Exploration

“…By the early 2020s, organoid intelligence had become a key player in personalized medicine [18]. It marks a significant step toward functional and implantable organ-like forms, wherein treatments can be specifically designed and tested to suit an individual's unique genetic makeup [5][6][7][8][9][10][13][14][15][16][17][18][19][20][21][22][23]35,62,[94][95][96][97][98].…”

Research progress on the application of organoids in gynecological tumors

“…Some studies have explored new sources of organization and cultivation methods. For example, Katcher et al collected endometrial samples from patients and used the matrix gel method to establish cancerous and normal endometrial tissue organoids for further analysis for DNA and RNA extraction and histological analysis ( Katcher et al, 2023 ). Hsin et al used xenografts (PDX) from patients with EC to construct organoids.…”