Establishing repeatability and ruggedness of chiral separations in micellar electrokinetic chromatography mass spectrometry: Combined use of covalently bonded capillary column and molecular micelles

Akter, Ferdoushi; Shamsi, Shahab A.

doi:10.1016/j.chroma.2019.460835

Cited by 8 publications

(8 citation statements)

References 19 publications

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“…CE is a useful method for drug discovery and development in various aspects (e.g., determinations of active ingredients, counterions, and impurities [138,139], enantiomeric separation [139][140][141][142][143][144][145][146], screenings of pK a , and determination of log p values [147][148][149], etc.). Challenges in producing sensitive and reliable quantitative data depend on the instrumentation setup, sample pretreatment/preconcentration, and hyphenated with suitable detectors.…”

Section: Future Trendsmentioning

confidence: 99%

Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Suntornsuk

Anurukvorakun

2022

Electrophoresis

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This review aims to illustrate sensitivity enhancement methods in capillary electrophoresis (CE) and their applications for pharmaceutical and related biochemical substance analyses. The first two parts of the article describe the introduction and principle of CE. The main part focuses on strategies for sensitivity improvement in CE including detector and capillary technologies and preconcentration techniques. Applications of these techniques for pharmaceutical and biomedical substance analyses are surveyed during the years 2018-2021.

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Section: Future Trendsmentioning

confidence: 99%

Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Suntornsuk

Anurukvorakun

2022

Electrophoresis

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“…In the past decade, several MS-compatible polymeric surfactants have been used. The most frequently used surfactants suitable for chiral MEKC-MS are AA and peptide-based polymeric surfactant [54][55][56], sugar-based polymeric surfactants [57], mixtures of polymeric surfactants [23], and achiral volatile surfactants, such as ammonium perfluorooctanoate (APFO) [58,59]. APFO is an achiral, but MS-compatible, volatile surfactant used in a system involving indirect chiral separation employing chiral derivatizing reagents (Table 2)*.…”

Section: Chiral Selectors In Mekc-msmentioning

confidence: 99%

“…Much of the past 10 years of research for direct chiral separation in MEKC-MS has been focused on amino acid or dipeptide-based polymers derived from undecylenic acid, with carbamate and amide linkages functionalized with single amino acid or dipeptide head group [23,[54][55][56]. Using 25 mM poly-sodium N-undecenoyl-L,L-leucylvalinate (poly L,L-SULV) as a chiral pseudophase in MEKC-MS baseline enantioseparation of warfarin, its five metabolites along with the internal standard (coumachlor) was obtained in 45 min [54].…”

Section: Direct Chiral Separations In Mekc-msmentioning

confidence: 99%

“…Furthermore, the fused-silica capillary tip lacks robustness, and electrospray erosion limits the capillary column's lifetime. To overcome these challenges, Akter et al recently proposed the covalently bonded 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) column filled with polysodium N-undecenoxy carbonyl-L-leucinate (poly-L-SUCL) [56]. Simultaneous enantiomeric separations and MS/MS detection of three β-blockers were achieved within 25 min with an improved column lifetime of at least 45-50 runs.…”

Section: Direct Chiral Separations In Mekc-msmentioning

confidence: 99%

“…A drug that can be obtained only using a physician's prescription is considered a prescription drug. Four prescription drugs (metoprolol (METOP), atenolol (ATEN), duloxetine (DX), and methyldopa (MDOPA)), in the form of tablets or capsules, were analyzed in three separate reports [35,38,56]. Duloxetine (also known as Cymbalta) is a selective serotonin and norepinephrine reuptake inhibitor.…”

Section: Pharmaceuticalsmentioning

confidence: 99%

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Capillary Electrophoresis Mass Spectrometry: Developments and Applications for Enantioselective Analysis from 2011–2020

Shamsi

Akter

2022

Molecules

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It is now more than 25 years since the first report of enantioselective analysis by capillary electrophoresis-mass spectrometry (CE-MS) appeared. This article reviews the power of chiral CE-MS in resolving issues on the use of chiral selector incompatibility with MS and poor detectability encountered for chiral compounds by UV detection. The review begins with the general principles, requirements, and critical aspects of chiral CE-MS instrumentation. Next, the review provides a survey of MS-compatible chiral selectors (CSs) reported during the past decade, and the key achievements encountered in the time period using these CSs. Within the context of the strategies used to combine CE and MS, special attention is paid to the approaches that feature partial filling technique, counter-migration techniques, and direct use of CS, such as molecular micelles. In particular, the development and application of moving and fixed CS for EKC-MS, MEKC-MS, and CEC-MS demonstrate how various chiral compounds analyses were solved in a simple and elegant way during the 2010–2020 review period. The most noteworthy applications in the determination of chiral compounds are critically examined. The operating analytical conditions are detailed in the Tables, and the authors provide commentary on future trends of chiral separations by CE-MS.

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Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

et al. 2022

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We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. To this end, we use surface modification of the separation fused silica capillary by the covalently attached copolymer of acrylamide (AM) and 2‐acrylamido‐2‐methyl‐1‐propanesulfonate (AMPS), that is, poly(AM‐co‐AMPS) or PAMAMPS. Coatings were formed by the in‐capillary polymerization of a mixture of the neutral AM and anionic AMPS monomers premixed in various ratios in order to control the charge density of the copolymer. EOF mobility varies in the 0 to ∼40 × 10−9 m2 V−1 s−1 interval for PAMAMPS coatings ranging from 0 to 60 mol.% of charged AMPS monomer. For EOF in PAMAMPS‐treated capillaries, we observed (i) a negligible dependence on pH in the 2–10 interval, (ii) a minor variance among background electrolytes (BGEs) in function of their components and (iii) its standard decrease with increasing ionic strength of the BGE. Interest in variable cathodic EOF was demonstrated by the amelioration of separation of two kinds of isomeric anionic analytes, that is, monosaccharides phosphates and helquat enantiomers, in counter‐EOF mode.

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Establishing repeatability and ruggedness of chiral separations in micellar electrokinetic chromatography mass spectrometry: Combined use of covalently bonded capillary column and molecular micelles

Cited by 8 publications

References 19 publications

Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Capillary Electrophoresis Mass Spectrometry: Developments and Applications for Enantioselective Analysis from 2011–2020

Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

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