“…Not all cows showed a change over time, indicating that the different diets did in some cases not affect the animal-specific emission of the milk. Some sample-specific variation occurred in some cases which could not be explained by the experiment's design, which were supposed to be related to the day time-specific condition of the cow [31] (it is known that variations in milk constituents may occur, despite controlled and constant diet and housing conditions [32,33]-the variation of DL may be related to this circumstance, too). In cases where the data indicated a trend over time, no constant diet effects were observed in the Mw1ye for these cows, so the time trend can be considered to be marginal in relation to diet, but in the Mw1w, the time trend of 11 cows was, at the same time, a diet effect with higher emission for the pure hay diet, thus, the diet effect was biased in this parameter by the time trend.…”