Establishment and Application of a Visual DNA Microarray for the Detection of Food-borne Pathogens

Li, Yongjin

doi:10.2116/analsci.32.215

Cited by 18 publications

(6 citation statements)

References 21 publications

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“…Main immunological detection includes ELISA and colloidal gold immunochromatographic test methods, the former detection limit is about 10 3 –10 6 CFU/ml, the detection time is 3–4 hr (Brandao, Liebana, & Pividori, 2015; Di Febo et al, 2019), the latter detection limit is 10 5 CFU/ml, the detection time is about 15 min (Duan et al, 2017); however, Salmonella pathogen often contaminates food only at low levels, which often resulted in negative results. Molecular biology methods have lower detection limits, especially PCR, the detection limit can be up to 10 2 CFU/ml (Alves, Hirooka, & de Oliveira, 2016; Y. J. Li, 2016), the detection time is 5 hr or so, but using the traditional PCR to detect microorganisms often needs extracting DNA, amplification and electrophoresis, and so on, and the operation are relatively complicated. Recently, digital PCR and real‐time PCR have been widely applied for Salmonella or other pathogens assay, they have rapid, quantitative, and low detection limit advantages, but expensive instrument disadvantage is also obvious (M. Wang et al, 2018; Zhou et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots

Xiang-yang

Liu

2020

Journal of Food Safety

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Salmonella pathogen is old and difficult to control in food safety. The study was aimed to develop a sensitive and rapid CdTe/ZnS quantum dots (QDs) mediated fluorescence-linked immunosorbent assay (FLISA) of Salmonella spp. The optical properties and structure of the CdTe/ZnS QDs were characterized by UV-Vis absorption spectroscopy and transmission electron microscopy. The emission peak wavelength is 575 nm. The CdTe/ZnS QDs-labeled secondary antibody (goat anti-rabbit IgG) was formed by biotin-avidin system. The detection limits of S. Enteritidis and artificial simulated samples are 1.0 × 10 3 CFU/ml and 1.78 × 10 3 CFU/ml, respectively. The study of FLISA stability shows that the antibody with QDs had very good activity when it was stored in the dark at 4 C for at least 35 days. The FLISA provides a new and easy-operated method for the rapid detection of Salmonella spp. and has broad application in food safety.

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Section: Resultsmentioning

confidence: 99%

Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots

Xiang-yang

Liu

2020

Journal of Food Safety

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“…These techniques can detect multiple strains/species of pathogens at one time. Implementations such as microarrays (Li, 2016), multiplex PCR (Rodríguez et al, 2015), and genome scale metabolic technique (Metz, Ding, & Baumler, 2018) have been developed. However, these techniques require sophisticated analytical equipment and are not well suited for routine or field detection.…”

Section: Comparison Of Test Methodsmentioning

confidence: 99%

Single and multiple detections of foodborne pathogens by gold nanoparticle assays

Pissuwan

Gazzana

Mongkolsuk

et al. 2019

WIREs Nanomed Nanobiotechnol

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A late detection of pathogenic microorganisms in food and drinking water has a high potential to cause adverse health impacts in those who have ingested the pathogens. For this reason there is intense interest in developing precise, rapid and sensitive assays that can detect multiple foodborne pathogens. Such assays would be valuable components in the campaign to minimize foodborne illness. Here, we discuss the emerging types of assays based on gold nanoparticles (GNPs) for rapidly diagnosing single or multiple foodborne pathogen infections. Colorimetric and lateral flow assays based on GNPs may be read by the human eye. Refractometric sensors based on a shift in the position of a plasmon resonance absorption peak can be read by the new generation of inexpensive optical spectrometers. Surface‐enhanced Raman spectroscopy and the quartz microbalance require slightly more sophisticated equipment but can be very sensitive. A wide range of electrochemical techniques are also under development. Given the range of options provided by GNPs, we confidently expect that some, or all, of these technologies will eventually enter routine use for detecting pathogens in food. This article is categorized under: Diagnostic Tools > Biosensing

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“…Most of methods are time consuming, laborious, and less specific (Abubakar et al, 2007;Kim et al, 2006;Jarvik et al, 2010). To overcome these limitations, various molecular and genetic-based approaches such as polymerase chain reaction (PCR) (Park et al, 2009;de Freitas et al, 2010;Liu et al,2012;He et al, 2016), sequence-based serotyping, and DNA microarray hybridization (Guard et al,2012;Li, 2016) methods have been developed. Recently, specific PCR based-markers have gained importance in pathogen detection due to fast detection and high accuracy in comparison to the conventional methods (Kim et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Multiplexed Stn and Plca based specific genetic marker for early detection of Salmonella enterica and Listeria monocytogenes in milk samples

Saini

Kaushal²,

Kumar

2020

Ann.UDJG.FoodTechnology

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In this study conventional PCR and multiplex PCR based method was developed for the detection of two common foodborne pathogens Salmonella enterica and Listeria monocytogenes in 20 raw milk samples. The PCR was undertaken to detect two genes namely, Salmonella enterotoxin (stn) gene and phosphatidylinositol-specific phospholipase C gene (plcA) from both the organisms. The DNA templates (for both organisms) were amplified using specific set of primers. The resulting amplicons were found to be 265 bp and 147 bp respectively. Validation studies were further performed in artificial spiked milk samples and raw milk samples using multiplex PCR. The available detection methods are bacterial culturing, biochemical tests, serological tests, antibiotic sensitivity, ELISA and PCR. All these methods are either expensive or non-confirmatory and have some limitations. The reported multiplexed PCR based genetic marker completes overall analysis in 80 min which is the minimum time reported so far for the confirmation of these foodborne pathogens. Sensitivity and specificity of developed method was calculated and compared with different conventional methods. The detection limit of the assay for the S. enterica was 6.6 x10 0 CFU/mL and for L. monocytogenes was 4.5x10 0 CFU/mL.

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Establishment and Application of a Visual DNA Microarray for the Detection of Food-borne Pathogens

Cited by 18 publications

References 21 publications

Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots

Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots

Single and multiple detections of foodborne pathogens by gold nanoparticle assays

Multiplexed Stn and Plca based specific genetic marker for early detection of Salmonella enterica and Listeria monocytogenes in milk samples

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