Establishment and application of a human osteosarcoma U-2OS cell line that can stably express Cas9 protein

Zhou, Yonghua; Li, Yingjie; Yi, Zilin; Jiang, Mo

doi:10.1007/s11010-022-04434-z

Cited by 2 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 65 The U 2 OS tumor cell line, first isolated from a sarcoma case in 1964, is a widely used cell line model in the pathogenesis of osteosarcoma. 66 Administration of 35 mg/kg/day of acrylamide by gastric gavage for 2 weeks to adult male rats resulted in a significant increase in serum TNF-α and leptin levels in the rats, compared with the control group. 67 Increased leptin levels in the blood (hyperleptinemia), observed as a comorbidity of obesity, 68 is associated with adipose tissue inflammation.…”

Section: Development Of the Obesogen Hypothesismentioning

confidence: 94%

From a toxin to an obesogen: a review of potential obesogenic roles of acrylamide with a mechanistic approach

Büyükdere

Akyol

2023

Nutrition Reviews

View full text Add to dashboard Cite

Obesity and obesity-related disorders such as cancer, type 2 diabetes, and fatty liver have become a global health problem. It is well known that the primary cause of obesity is positive energy balance. In addition, obesity is the consequence of complex gene and environment interactions that result in excess calorie intake being stored as fat. However, it has been revealed that there are other factors contributing to the worsening of obesity. The presence of nontraditional risk factors, such as environmental endocrine-disrupting chemicals, has recently been associated with obesity and comorbidities caused by obesity. The aim of this review was to examine the evidence and potential mechanisms for acrylamide having endocrine-disrupting properties contributing to obesity and obesity-related comorbidities. Recent studies have suggested that exposure to environmental endocrine-disrupting obesogens may be a risk factor contributing to the current obesity epidemic, and that one of these obesogens is acrylamide, an environmental and industrial compound produced by food processing, particularly the processing of foods such as potato chips, and coffee. In addition to the known harmful effects of acrylamide in humans and experimental animals, such as neurotoxicity, genotoxicity, and carcinogenicity, acrylamide also has an obesogenic effect. It has been shown in the literature to a limited extent that acrylamide may disrupt energy metabolism, lipid metabolism, adipogenesis, adipocyte differentiation, and various signaling pathways, and may exacerbate the disturbances in metabolic and biochemical parameters observed as a result of obesity. Acrylamide exerts its main potential obesogenic effects through body weight increase, worsening of the levels of obesity-related blood biomarkers, and induction of adipocyte differentiation and adipogenesis. Additional mechanisms may be discovered. Further experimental studies and prospective cohorts are needed, both to supplement existing knowledge about acrylamide and its effects, and to clarify its established relationship with obesity and its comorbidities.

show abstract

Section: Development Of the Obesogen Hypothesismentioning

confidence: 94%

From a toxin to an obesogen: a review of potential obesogenic roles of acrylamide with a mechanistic approach

Büyükdere

Akyol

2023

Nutrition Reviews

View full text Add to dashboard Cite

show abstract

“…These endogenous promoters are often used for high-efficiency expression of recombinant proteins and also be applied to the expression of Cas9 protein with the rise of the CRISPR/Cas9 system [ 25 ]. Moreover, in the expression of relevant elements of the CRISPR/Cas9 system, the strong Pol II promoter can also be used for the expression of resistance genes or marker genes, which ensures that the gene-edited monoclonal cells can be filtered for genetic researches by antibiotic treatment [ 26 ] or flow cytometry [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization and Application of CRISPR/Cas9 Genome Editing in a Cosmopolitan Pest, Diamondback Moth

Zhang

Xiong

Xie

et al. 2022

IJMS

View full text Add to dashboard Cite

The CRISPR/Cas9 system is an efficient tool for reverse genetics validation, and the application of this system in the cell lines provides a new perspective on target gene analysis for the development of biotechnology tools. However, in the cell lines of diamondback moth, Plutella xylostella, the integrity of the CRISPR/Cas9 system and the utilization of this cell lines still need to be improved to ensure the application of the system. Here, we stabilize the transfection efficiency of the P. xylostella cell lines at different passages at about 60% by trying different transfection reagents and adjusting the transfection method. For Cas9 expression in the CRIPSPR/Cas9 system, we identified a strong endogenous promoter: the 217-2 promoter. The dual-luciferase and EGFP reporter assay demonstrated that it has a driving efficiency close to that of the IE1 promoter. We constructed pB-Cas9-Neo plasmid and pU6-sgRNA plasmid for CRISPR/Cas9 system and subsequent cell screening. The feasibility of the CRISPR/Cas9 system in P. xylostella cell lines was verified by knocking out endogenous and exogenous genes. Finally, we generated a transgenic Cas9 cell line of P. xylostella that would benefit future exploitation, such as knock-in and multi-threaded editing. Our works provides the validity of the CRISPR/Cas9 system in the P. xylostella cell lines and lays the foundation for further genetic and molecular studies on insects, particularly favoring gene function analysis.

show abstract

Establishment and application of a human osteosarcoma U-2OS cell line that can stably express Cas9 protein

Cited by 2 publications

References 35 publications

From a toxin to an obesogen: a review of potential obesogenic roles of acrylamide with a mechanistic approach

From a toxin to an obesogen: a review of potential obesogenic roles of acrylamide with a mechanistic approach

Optimization and Application of CRISPR/Cas9 Genome Editing in a Cosmopolitan Pest, Diamondback Moth

Contact Info

Product

Resources

About