2017
DOI: 10.1007/s11627-017-9864-z
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Establishment and biochemical characterization of tamarillo (Solanum betaceum Cav.) embryogenic cell suspension cultures

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Cited by 10 publications
(9 citation statements)
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“…The integration of fractionation and/or type-enrichment for proteins or peptides, including resolving protein homogenate by 1D-PAGE followed by in-gel digestion and LC-MS/MS antibody-based acetylated-lysine enrichment ( Zhao et al, 2015a , b ; dos Santos et al, 2016 ; Xia et al, 2016 ; Alves et al, 2017 ), may provide new insights and in-depth proteome coverage during the induction of SE. However, few of the exquisite variety of proteomic tools have been implemented so far.…”
Section: Plant Proteomics Technologiesmentioning
confidence: 99%
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“…The integration of fractionation and/or type-enrichment for proteins or peptides, including resolving protein homogenate by 1D-PAGE followed by in-gel digestion and LC-MS/MS antibody-based acetylated-lysine enrichment ( Zhao et al, 2015a , b ; dos Santos et al, 2016 ; Xia et al, 2016 ; Alves et al, 2017 ), may provide new insights and in-depth proteome coverage during the induction of SE. However, few of the exquisite variety of proteomic tools have been implemented so far.…”
Section: Plant Proteomics Technologiesmentioning
confidence: 99%
“…Biochemical analyses of the proteolytic activity reveal that serine proteases, aspartic proteases, and metalloproteases are dominant in EC, whereas serine proteases dominate NEC in Solanum betaceum ( Alves et al, 2017 ). Leucine aminopeptidase has been found to be unique to PEG-dependent somatic embryo maturation of C. papaya ( Vale et al, 2014 ).…”
Section: Proteolytic Enzymes and The Ubiquitin Proteasome Systemmentioning
confidence: 99%
“…The second group of substrates ( Figure 5 d–f) was used to evaluate the presence of glycoside hydrolases (MU-G, MU-NAG, and MU-C) and alkaline phosphatases (MU-P). The choice of these substrates is based on previous works with tamarillo cell suspension cultures [ 13 ].…”
Section: Resultsmentioning
confidence: 99%
“…Analysis of ICL cellular protein profiles revealed high expression levels of stress-related proteins [ 11 , 12 ], confirming the role of tissue culture systems in inducing molecular reprogramming. During recent experiments, S. betaceum ICL was grown efficiently as PCSs [ 13 ]. The evaluation of their extracellular protein patterns showed that cell suspensions of tamarillo produce and secrete a wide array of proteins into the culture medium, including endoglucanases, proteases, and low molecular weight (>20 kDa) peptides [ 13 ].…”
Section: Introductionmentioning
confidence: 99%
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