Establishment and characterization of a murine Gardner's osteosarcoma cell line (GOS/T):Purification of bone-specific alkaline phosphatase (ALPase) and use of anti-ALPase as a diagnostic aid

Three osteosarcomas (OS) with features resembling malignant fibrous histiocytoma (MFH) were selected and investigated to identify any clinico-pathological similarities. In all cases there was no significant difference from conventional OS on the radiography and laboratory data. The appearance of MFH-like features within the whole tumour tissue varied from 7% to 55%. It was composed of spindle-shaped cells arranged in short irregular fascicles and a storiform pattern admixed with osteoclast-like giant cells, but devoid of neoplastic osteoid. Such spindle-shaped cells had a poorly developed rough endoplasmic reticulum and expressed a strong alkaline phosphatase activity as well as vimentin. A series of allografts to athymic mice using the MFH-like tissues also showed histologically a proliferation of plump spindle-shaped cells with a storiform pattern lacking osteoclast-like giant cells, and intensely positive for alkaline phosphatase. These findings indicate that the MFH-like features are identified as modulated OS. The constituting cells are most likely to be poorly developed with possible phenotypic alteration in the maturation stage of osteoblastic cell lineage, but different from conventional MFH of bone as regards their distinct histochemical pattern.

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Osteosarcoma with features mimicking malignant fibrous histiocytoma

Yoshida

Yumoto

Minamizaki

1992

Vichows Archiv A Pathol Anat

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Osteosarcoma with Prominent Epithelioid Features

Yoshida

Yumoto

Adachi

et al. 1989

Acta Pathologica Japonica

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Osteosarcoma in the metaphysis to epiphysis of the left femur of a 17 year old male is reported. The lesion appeared osteolytic with sclerotic foci on roentgenographs, accompanied by an extensive tumor shadow in the surrounding soft tissue. While 60% of the tumor was necrotic, histological examination of the remaining viable tissue revealed that it consisted almost entirely of a sheet of epithelioid cells, separated by thin, fibrovascular septa with an alveolar‐like pattern, suggestive of metastatic carcinoma. Only a few areas were characterized by malignant osteoid tissue intermingled with the above cells, showing significant positivity for bone‐specific alkaline phosphatase and 5 nucleotidase, thus permitting a diagnosis of osteosarcoma. Autopsy findings revealed that the metastatic foci were histologically similar to those of the primary tumor. Electron microscopy revealed poor development of cytoplasmic organelles, supporting possible derivation from an osteoblastic cell lineage at an early stage. Acta Pathol Jpn 39: 439 445, 1989.

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Establishment and characterization of a murine Gardner's osteosarcoma cell line (GOS/T):Purification of bone-specific alkaline phosphatase (ALPase) and use of anti-ALPase as a diagnostic aid

Cited by 2 publications

References 21 publications

Osteosarcoma with features mimicking malignant fibrous histiocytoma

Osteosarcoma with features mimicking malignant fibrous histiocytoma

Osteosarcoma with Prominent Epithelioid Features

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