Establishment and characterization of a macrophage cell line from the goldfish

Wang, R

doi:10.1006/fsim.1995.0032

Cited by 87 publications

(57 citation statements)

References 23 publications

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“…A number of studies production of ROM as evidenced by NBT reduction in have previously shown activity due to esterases and/or leucocytes. Although this reaction was relatively low acid phosphatases (Meseguer et al 1994, Dalmo & Sel-compared to the action of zymosan, the possibility that jelid 1995, Wang et al 1995, Afonso et al 1997, ROM could be a part of the host response against gyro- Ssrensen et al 1997) in fish leucocytes.…”

Section: Discussionmentioning

confidence: 98%

Rainbow trout leucocyte activity:influence on the ectoparasitic monogenean Gyrodactylus derjavini

Buchmann¹,

Bresciani²

1999

Dis. Aquat. Org.

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The ectoparasitic monogenean Gyrodactylus deqavinl from ralnbow trout Oncorhynchus mykiss was exposed in vltro to macrophages isolated as pentoneal exudate cells or as pronephros cells from the host Cells colonized the parasite especially in the mannose-nch regions in the c e p h a l~c ducts where ciliated structures were abundant Opsonizatlon wlth fresh serum, in contrast to heatinactivated serum, enhanced colonlzat~on also on other body parts The adverse effect of the activated macrophages towards G derjavlnl was assoc~ated with a heat-lab~le component released from these cells to the culture medium Analysis of substances released from the cells showed leactivity for a number of enzymes, complement factor C3, ~nterleukin (11-1) and reactive oxygen metabohtes Chemotaxls assays with pronephnc leucocytes showed chemoattractants in G derlavini and the respiratory burst level of macrophages was slightly elevated due to parasite exposure It is suggested that skln leucocytes contribute to an increased level of complement factors in the trout skln during the host response, whereby a hostlle m~croenvironment for the parasites IS created In addition, the 1L-l production could affect mucous cell secretion and hyperplasia and add to the antiparasitlc actlon of the epithelium Likewise react~ve oxygen metabolites and vanous enzymes are l~k e l y to be involved In the skin response

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Section: Discussionmentioning

confidence: 98%

Rainbow trout leucocyte activity:influence on the ectoparasitic monogenean Gyrodactylus derjavini

Buchmann¹,

Bresciani²

1999

Dis. Aquat. Org.

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“…It has recently been shown that fish macrophages possess the inducible form of NOS, and that they are able to release NO after stimulation with certain molecules (Wang et al 1995, Laing et al 1999). The first experiments carried out to compare the bactericidal properties of NO and peroxynitrite suggested the latter molecule possessed a higher toxicity than NO for Escherichia coli (Brunelli et al 1995, using SIN-1 and gaseous NO), Candida albicans (Vazquez-Torres et al 1995, using SIN-1 and chemically synthesized peroxynitrite and sodium nitroprusside as NO generator) and Brugia malayi (Thomas et al 1997, using SIN-1 and SNAP).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, work by Neumann et al (1995), Wang et al (1995) and Neumann & Belosevic (1996) has shown that a macrophage cell line and primary macrophage cultures from goldfish produce NO when stimulated with macrophage activating factor (MAF) and bacterial lipopolysaccharide (LPS). Wiegertjes et al (1998) reported NO production in vitro by carp macrophages stimulated with LPS and with the intracellular parasite Trypanosoma borrelli.…”

Section: Introductionmentioning

confidence: 99%

Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish

Campos-Pérez¹,

Ellis²,

Secombes³

2000

Dis. Aquat. Org.

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The inhibitory effect of the nitric oxide (NO) donor S-nitroso-acetyl-penicillamine (SNAP) and the NO and O 2 -donor 3-morpholino-sydnonimine hydrochloride (SIN-1) was tested in a cell-free assay. Strains of the bacterial fish pathogens Aeromonas salmonicida, Renibacterium salmoninarum and Yersinia ruckeri were exposed to different concentrations of the NO donors for 24 h. The results showed that NO possesses inhibitory properties, while peroxynitrite had no effect. However, when SIN-1 was used in combination with superoxide dismutase (SOD) alone or with catalase, an inhibitory effect comparable to that caused by SNAP was seen. The implications of these results are discussed. KEY WORDS: SNAP · SIN-1 · Nitric oxide · Peroxynitrite · Renibacterium salmoninarum · Aeromonas salmonicida · Yersinia ruckeri Resale or republication not permitted without written consent of the publisherDis Aquat Org 43: [109][110][111][112][113][114][115] 2000 of non-infectious conditions such as cerebral ischaemia (Forman et al. 1998), acute myocardial infarction (Shimojo et al. 1998) and heart injury (Wang & Zweier 1996). Moreover, endogenously produced peroxynitrite induces protein oxidation in the mitochondria and nucleus of immunostimulated macrophages (Szabo et al. 1997). Hence, it is not clear whether peroxynitrite formation is beneficial for the host even though it inhibits microbes.In fish, NOS activity has been reported in several tissues (Schoor & Plumb 1994, Sodestrom et al. 1995). Moreover, work by Neumann et al. (1995), Wang et al. (1995) and Neumann & Belosevic (1996) has shown that a macrophage cell line and primary macrophage cultures from goldfish produce NO when stimulated with macrophage activating factor (MAF) and bacterial lipopolysaccharide (LPS). Wiegertjes et al. (1998) reported NO production in vitro by carp macrophages stimulated with LPS and with the intracellular parasite Trypanosoma borrelli. Buentello & Gatlin (1999) also showed that medium composition exerts an influence in the NO production of channel catfish macrophages stimulated by LPS. The inducible form of NOS, iNOS, has been cloned and partially sequenced from a goldfish macrophage cell line and primary cultures of rainbow trout macrophages (Laing et al. 1999). Previous experiments have demonstrated that rainbow trout injected with Renibacterium salmoninarum showed an increase of NO (as measured by nitrite/nitrate concentration) in serum, and that iNOS is expressed in kidney and gill tissue (CamposPérez et al. 2000), suggesting that the produced NO may have a role in defence in this organism. Hence, in the present work the effectiveness of NO and peroxynitrite to inhibit strains of bacterial pathogens known to infect rainbow trout (Aeromonas salmonicida, R. salmoninarum and Yersina ruckeri) is assessed by using the NO donor S-nitroso-acetyl-penicillamine (SNAP) and the NO and O 2 -donor 3-morpholino-sydnonimine hydrochloride (SIN-1) in a cell-free assay. MATERIALS AND METHODSBacteria. The species and strains used were Aeromonas salmon...

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“…As células do sistema imune são os leucócitos, células derivadas da medula óssea que incluem células mielóides (neutrófilos polimorfonucleares -PMNs, monócitos, eosinófilos e basófilos) e linfócitos (BROWM, 1997 WANG et al, 1995).…”

Section: 32) Migração Celularunclassified

Estresse de transporte em cascudos amazônicos ornamentais Cochliodon sp. (L145) e Hypostomus sp. (L28) (LORICARIIDAE)

et al. 2016

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In this study was evaluated the hematological response of ornamental Amazon plecos Cochliodon sp. (L145) and Hypostomus sp. (L28) submitted to transportation conditions. The erythrogram, leukogram, thrombogram, glucose and total plasma proteins (PPT) were determined at 0, 6, 24, 48 and 72 hours after 3-hours simulated transportation. For basal hematological profile, the blood was collected immediately to allow comparisons after the transport stress. Cochliodon sp. showed reduction in total erythrocyte after 6 and 24 hours of post-transport, and increase after 6 hours. Hypoglycemia were observed at 6 hours post-transport for Hypostomus sp. Thus, the Hypostomus sp. showed higher resistance compared to Cochliodon sp. when to the transport stress.

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Establishment and characterization of a macrophage cell line from the goldfish

Cited by 87 publications

References 23 publications

Rainbow trout leucocyte activity:influence on the ectoparasitic monogenean Gyrodactylus derjavini

Rainbow trout leucocyte activity:influence on the ectoparasitic monogenean Gyrodactylus derjavini

Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish

Estresse de transporte em cascudos amazônicos ornamentais Cochliodon sp. (L145) e Hypostomus sp. (L28) (LORICARIIDAE)

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