Establishment and characterization of a new cell line TC-YIK originating from argyrophil small cell carcinoma of the uterine cervix integrating HPV16 DNA

Abstract

A new cell line, designated TC-YIK, was established from YIK-1 tumor cells, derived from argyrophil small cell carcinoma (ASCC) of the uterine cervix, and serially heterotransplanted into nude mice, integrating human papillomavirus type 16 (HPV16) DNA. The population doubling time of TC-YIK was approximately 21.6 hours at the 119th subculture. Subcutaneous injection of 1 x 10(8) TC-YIK cells into nude mice yielded a solid tumor. The cytologic appearance of TC-YIK was similar to that of YIK-1. The TC-YIK cells contained argyrophil granules and neurosecretory granules in the cytoplasm and showed positive immunohistochemical staining for neuron-specific enolase, serotonin, and chromogranin. Thus, TC-YIK retained the histochemical characteristics of ASCC. The TC-YIK cells contained HPV16 DNA in a multiple-copy integrated form and actively transcribed the integrated HPV16 genome. Amplification of the c-myc oncogene was observed in the TC-YIK cells. These data suggest that TC-YIK is a useful in vitro experimental model of ASCC and that HPV16 and c-myc may play some role in the genesis of this malignant tumor and/or maintenance of the transformed TC-YIK phenotype.

Radiation sensitivity assay with a panel of patient‐derived spheroids of small cell carcinoma of the cervix

“…SCCC is a rare cancer with a poor prognosis and biological features that are not well elucidated. As a research platform, even conventional cell lines are quite rare . We demonstrated here that CTOS lines of SCCC were easily and efficiently established from surgical samples and used them as an example, applying radiation therapy technology.…”

“…As a research platform, even conventional cell lines are quite rare. 28,29 We demonstrated here that CTOS lines of SCCC were easily and efficiently established from surgical samples and used them as an example, applying radiation therapy technology. The success rates of CTOS preparation and culture are 98.7% in colorectal cancer, 16 80.0% in lung cancer 18 and 84.2% in bladder cancer.…”

Differentiation of a Cell Line of Human Cervical Argyrophil Small Cell Carcinoma to Macrophage Lineage Cells

“…[8][9][10] In our previous studies, we found that TC-YIK cells retained the histochemical characteristics of ASCC cells and could be used as an in vitro experimental model of ASCC, and that the TC-YIK cells were possibly of neuroendocrine origin, based on electron microscopic evidence of small, electron-dense, membrane-bound neurosecretory-type granules and immunohistochemical evidence of neuron-specific enolase, chromogranin, serotonin and gastrin. 8,9,11) In terms of the origin of ASCC, however, there have been three theories: ASCC originates from (i) amine precursor uptake and decarboxylation (APUD) cells, 2,3,12) (ii) undifferentiated epithelial cells with multi-differentiation ability, 13) or (iii) stem cells. …”

“…Isolation and characterization of TC-YIK cells are described elsewhere. 9,10) TC-YIK cells were cloned twice after 60 cell generations from the start of culture using the limiting dilution method. The clone that showed the most stable growth was used for the current study.…”

Effect of dibutyryl cyclic amp on morphologic features and marker production of human cervical argyrophil small‐cell carcinoma cell line

“…Therefore, development of in vivo and in vitro experimental systems is desirable not only for examining the biologic behavior of ASCC, but also for establishing an effective clinical treatment for the tumor. We reported previously the establishment and Characterization of an ASCC tumor line in nude mice and also a cell line from the tumor, designated TC-YIK (Ichimura et al, 1988(Ichimura et al, , 1991. In our previous study, we briefly rcportcd TC-YIK cells to be possibly of neuroendocrine origin based on the demonstration of small, electron-dense, membrane-bound neurosecretory-type granules by an electron microscopic study and positive staining for neuron-specific enolase, chromogranin, serotonin and gastrin by an immunohistochemical study (Ichimura ef a/., 1991).…”