Establishment and characterization of a novel alveolar rhabdomyosarcoma cell line, NCC-aRMS1-C1

Sin, Yooksil; Yoshimatsu, Yuki; Noguchi, Rei; Tsuchiya, Ryuto; Sei, Akane; Ono, Takuya; Toki, Shunichi; Kobayashi, Eisuke; Arakawa, Ayumu; Sugiyama, Masanaka; Yoshida, Akihiko; Kawai, Akira; Kondo, Tadashi

doi:10.1007/s13577-020-00403-0

Cited by 8 publications

(5 citation statements)

References 36 publications

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“…Short tandem repeats (STRs) were examined at 10 loci using the GenePrint 10 system (Promega, Madison, WI, USA), as previously described [ 32 ]. Genomic DNAs of the established cell line and the original tumor were prepared for PCR using AllPrep DNA/RNA Mini kits (Qiagen, Hilden, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Invasive potential was assessed using a real-time cell analyzer (xCELLigence, Agilent, Santa Clara, CA, USA) along with NCC-MFS1-C1 [ 28 ], NCC-MFS2-C1 [ 29 ], NCC-MFS3-C1 [ 30 ], and NCC-MFS4-C1 [ 31 ], as previously reported [ 32 ]. We seeded cells (4 × 10 4 ) suspended in a serum-free DMEM/F12 medium in the upper chamber coated with BD Matrigel matrix (BD Biosciences, Franklin Lakes, NJ, USA) ( n = 2).…”

Section: Methodsmentioning

confidence: 99%

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Establishment and Characterization of NCC-MFS5-C1: A Novel Patient-Derived Cell Line of Myxofibrosarcoma

Tsuchiya

Yoshimatsu²,

Noguchi³

et al. 2022

Cells

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Myxofibrosarcoma (MFS) is a highly aggressive malignancy with complex karyotypes and a postoperative recurrence tendency, owing to its strong invasiveness. Although systemic chemotherapy is considered in patients with unresectable MFS, the efficacy of conventional chemotherapy is hitherto unclear. Recently, drug screening analysis using a large number of tumor cell lines has been attempted to discover novel therapeutic candidate drugs for common cancers. However, the number of MFS cell lines is extremely small because of its low incidence—this hinders the conduction of screening studies and slows down the development of therapeutic drugs. To overcome this problem, we established a novel MFS cell line, NCC-MFS5-C1, which was shown to harbor typical MFS genetic abnormalities and thus had useful properties for in vitro studies. We conducted the largest integrated screening analysis of 210 drugs using NCC-MFS5-C1 cells along with four MFS cell lines, which we previously reported. Bortezomib (a proteasome inhibitor) and romidepsin (a histone deacetylase inhibitor) showed stronger antitumor effects than the standard drug, doxorubicin. Therefore, the NCC-MFS5-C1 cell line can potentially contribute to elucidating MFS pathogenesis and developing a novel MFS treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Establishment and Characterization of NCC-MFS5-C1: A Novel Patient-Derived Cell Line of Myxofibrosarcoma

Tsuchiya

Yoshimatsu²,

Noguchi³

et al. 2022

Cells

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“…PXD029225; spheroid of NCC-aRMS1-C1 cells with decellularized muscle extracts with 10% content, in the ProteomeXchange Consortium (https://proteomeconsortium.org) via the jPOST partner repository (https://repository.jpostdb.org) as muscle, and novel therapeutic approaches are required for improving clinical outcomes 4) . In our previous study, we established a novel cell line of alveolar rhabdomyosarcoma using surgically resected tissues and reported its ability for spheroid formation 5) . Although the cell line was well characterized, it was maintained under conventional tissue culture conditions.…”

Section: Data Availabilitymentioning

confidence: 99%

“…We prepared decellularized muscle extracts using mouse muscle tissues, which were used for the tissue culture of rhabdomyosarcoma cells. Rhabdomyosarcoma is a malignant sarcoma, often originating in the previous study 5) . Cells were maintained under tissue culture conditions according to the methodology used in our previous study 5) , and the decellularized muscle extracts was added to tissue culture at a volume concentration of 10%.…”

Section: Introductionmentioning

confidence: 99%

“…Rhabdomyosarcoma is a malignant sarcoma, often originating in the previous study 5) . Cells were maintained under tissue culture conditions according to the methodology used in our previous study 5) , and the decellularized muscle extracts was added to tissue culture at a volume concentration of 10%. Cells were seeded in an ultra-low attachment plate (Nunclon Sphera, Thermo Fisher, Waltham, MA, USA) with or without the decellularized muscle extracts at the concentration of 1×10 5 cells/well.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic analysis of spheroids of rhabdomyosarcoma cells cultured with decellularized muscle extracts

et al. 2022

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The tumor microenvironment determines tumor characteristics, and its recapitulation via in vitro tissue culture conditions is necessary for better understanding of molecular mechanisms of tumor behaviors and development of novel therapy. Rhabdomyosarcoma is a mesenchymal tumor that originates limb muscles. To assess the possible utility of the decellularized materials that contain native components of the microenvironment for the optimal humanized spheroid formation, we demonstrated co-culture of decellularized muscle extracts and rhabdomyosarcoma cells. The decellularized muscle extracts were prepared using a detergent, solubilized via pepsin digestion, and used for the spheroid culture of rhabdomyosarcoma cells in a low-attachment tissue culture plate. Here, we confirmed that these extracts contained proteins whose expression was unique to muscles. When rhabdomyosarcoma cells formed spheroids with decellularized muscle extracts, they exhibited heterogeneous morphology and a unique protein expression pattern. We conclude that in vitro cancer models established using decellularized tissue extracts from the originating organ have the potential to recapitulate features of in vivo tumor cells by providing the components of the tumor microenvironment. The precise molecular mechanisms for the interaction of cells and decellularized tissues are worth further investigation.

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Establishment and characterization of NCC-SS4-C1: a novel patient-derived cell line of synovial sarcoma

Tsuchiya

Yoshimatsu²,

Noguchi³

et al. 2021

Human Cell

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Establishment and characterization of a novel alveolar rhabdomyosarcoma cell line, NCC-aRMS1-C1

Cited by 8 publications

References 36 publications

Establishment and Characterization of NCC-MFS5-C1: A Novel Patient-Derived Cell Line of Myxofibrosarcoma

Establishment and Characterization of NCC-MFS5-C1: A Novel Patient-Derived Cell Line of Myxofibrosarcoma

Proteomic analysis of spheroids of rhabdomyosarcoma cells cultured with decellularized muscle extracts

Establishment and characterization of NCC-SS4-C1: a novel patient-derived cell line of synovial sarcoma

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