Establishment and Characterization of a Human Malignant Fibrous Histiocytoma Cell Line

Yonemoto, Tsukasa; Takenouchi, Toshinao; Tokita, Hisashi; Tatezaki, Shin-ichiro; Mukaida, Naofumi; Mikata, Atsuo; Moriya, Hideshige

doi:10.1097/00003086-199511000-00027

Cited by 4 publications

(10 citation statements)

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“…Cultured cell lines established from human MFHs produced TNF-a, interleukin (IL)-6, IL-8, and macrophage-colony stimulating factor (M-CSF). 19,42 Although possible productions of various factors in histiocytic KJ-A cells or in fibroblastic cells to compare with the histiocytic cell line should be further investigated, inflammatory cytokines released by neooplastic cells might be related to the complicated morphogenesis of MFH. 36 In conclusion, the present study demonstrated that in vivo and in vitro tumor lines (KJ, KJ-P, and KJ-A) established from a rat subcutaneous MFH possessed histiocytic and macrophage-like characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…It has been considered that undifferentiated stem cells seen occasionally in human MFHs may have a potential for multidirectional differentiation towards osteoblasts, myoblasts, and lipoblasts. 9,17,23,27,41,42 Thus, the latest WHO (2002) classification for human soft tissue tumors considers MFH a synonym for undifferentiated pleomorphic sarcomas. 8 The present study revealed no evidence that neoplastic cells differentiating into myogenic cells (no expression of desmin and a-SMA, and no reaction with PTAH stain), neurogenic cells (no expression of S-100 protein), osteogenic cells (no reactivity for ALP), or lipoblastic cells (no reaction with oil red O stain) were present.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a Rat Subcutaneous Malignant Fibrous Histiocytoma and Its Tumor Lines, with Reference to Histiocytic Features

et al. 2007

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Malignant fibrous histiocytoma (MFH) is regarded as soft tissue-derived undifferentiated pleomorphic sarcoma, of which the histogenesis remains to be proven. To investigate the cellular characteristics, a homotransplantable tumor line (KJ) was established from a spontaneous MFH that developed in the subcutis of an aged F344 rat. KJ tumors have been produced in syngeneic rats by serial subcutaneous implantation of tissue fragments. The original and KJ tumors consisted of oval and fusiform cells arranged in interlacing bundles with fibrous stroma. Occasional giant cells with bizarre nuclei were observed. Enzyme/immunohistochemically, neoplastic cells reacted to ED1 and ED2 (antibodies specific for rat histiocytes/macrophages), and showed a positive reaction to vimentin and lysosomal enzyme markers such as acid phosphatase (ACP) and nonspecific esterase (Non-SE). Electron microscopically, neoplastic cells possessed lysosomal granules in cytoplasm. A cloned cell line (KJ-A) was isolated from a KJ tumor. KJ-A cells showed positive reactions to ED1, ED2, ACP, and Non-SE, and had cytoplasmic lysosomal granules. Tumors induced by KJ-A cells exhibited histologic and enzyme/immunohistochemical findings similar to those of KJ tumors. Lipopolysaccharide (LPS) treatment increased the number of ED1-positive cells and the expression of tumor necrosis factor-alpha mRNA by reverse transcription-polymerase chain reaction. Collectively, it is likely that rat MFH cells originally possess histiocyte/macrophage-like features that may be enhanced by LPS. Because tumor lines are useful for in vivo and in vitro studies concerning different characteristics of the original neoplasms. KJ and KJ-A should prove useful for studies concerning the morphogenesis of MFH.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of a Rat Subcutaneous Malignant Fibrous Histiocytoma and Its Tumor Lines, with Reference to Histiocytic Features

et al. 2007

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“…Although several investigators have studied MFH cell lines, and found positive immunoreactivity for type V collagen, 11,12 none have investigated the expression of collagen mRNA in an established MFH cell line using the RT–PCR technique. In our study, the TNMY1 cells expressed collagen types I, III and V. The level of expression of type V collagen mRNA in the human MFH cell line TNMY1 was essentially the same as that of type I collagen.…”

Section: Discussionmentioning

confidence: 99%

“…Some of the confusion regarding MFH is attributable to the fact that the origin of this tumor is still unclear, due to its pleomorphic appearance. Several researchers have reported the establishment of cell lines of MFH to try to elucidate the pathogenesis of MFH 7–15 . The results have not been consistent with each other and thus the pathogenesis of MFH is still being debated.…”

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confidence: 99%

Establishment and characterization of cell line TNMY1 derived from human malignant fibrous histiocytoma

Nakatani

Marui

Yamamoto

et al. 2001

Pathology International

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Although malignant fibrous histiocytoma (MFH) is one of the most common soft tissue sarcomas, its pathogenesis remains unclear. In this study, a cell line derived from human MFH, TNMY1, was established from a metastatic chest-wall lesion of a 60-year-old woman with MFH. The TNMY1 cell line was passaged 95 times, and it still retained the biological characteristics of the original tumor. TNMY1 consists of spindle-shaped cells and pleomorphic cells associated with multinucleated giant cells. Immunohistochemical studies showed that the spindle-shaped and pleomorphic cells were positive for vimentin, CD68 and alpha-smooth muscle actin, but negative for epithelial membrane antigen, desmin, muscle actin, alpha-sarcomeric actin, myoglobin, lysozyme and S-100 protein. The cells expressed collagen types I, III and V. These results indicate that MFH may originate from mesenchymal stem cells with the potential to differentiate into either fibroblasts or histiocytes. An elevated level of collagen type V mRNA expression is considered to support a diagnosis of MFH.

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“…The transplanted tumors derived from cases 2 and 3 contained abundant bizarre multinucleated giant cells of human origin. Yonemoto et al established a cloned MFH cell line, in which multinucleated giant cells appeared to be stable 39 . Using immunocytochemical staining by proliferating cell nuclear antibody (PCNA), they demonstrated that the cultured multinucleated giant cells have proliferating ability.…”

Section: Discussionmentioning

confidence: 99%

Origin of histiocyte‐like cells and multinucleated giant cells in malignant fibrous histiocytoma: Neoplastic or reactive?

Hatano¹,

Tokunaga²,

Ogose³

et al. 1999

Pathology International

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The origin of histiocyte-like cells in malignant fibrous histiocytoma (MFH) remains controversial. To determine whether histiocyte-like cells and multinucleated giant cells show reactive or neoplastic proliferation, we transplanted human storiform-pleomorphic MFH to nude mice and investigated the origin of histiocyte-like cells using the DNA in situ hybridization (ISH) system. In addition, we analyzed the mRNA expression of mouse c-fms and human colony stimulating factor-1 (CSF-1); immunohistochemical expression of markers detectable in cells of monocyte/macrophage lineage. The DNA ISH revealed neoplastic proliferation of fibroblastic cells and bizarre multinucleated giant cells of human origin. Monocyte/macrophage lineage cells were seen in parental tumors, whereas they did not participate in neoplastic proliferation in transplanted tumors. The parental tumors expressed human CSF-1 mRNA and the histiocyte-like cells in transplanted tumors expressed 'mouse' c-fms mRNA. These results suggest that MFH induce infiltration of monocyte/macrophage and CSF-1 is one of the mediators involved in this phenomenon, because the human CSF-1 can act as a ligand to the mouse c-fms. Histiocyte-like cells in MFH should be considered as a reactive monocyte/macrophage lineage rather than as an element of neoplasm.

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Establishment and Characterization of a Human Malignant Fibrous Histiocytoma Cell Line

Cited by 4 publications

References 0 publications

Characterization of a Rat Subcutaneous Malignant Fibrous Histiocytoma and Its Tumor Lines, with Reference to Histiocytic Features

Characterization of a Rat Subcutaneous Malignant Fibrous Histiocytoma and Its Tumor Lines, with Reference to Histiocytic Features

Establishment and characterization of cell line TNMY1 derived from human malignant fibrous histiocytoma

Origin of histiocyte‐like cells and multinucleated giant cells in malignant fibrous histiocytoma: Neoplastic or reactive?

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