Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

Abstract

Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal cancers worldwide. Considering their diversity, the establishment of new continuous CRC cell lines with clear genetic backgrounds will provide useful tools for exploring molecular mechanisms, screening and evaluating antitumor drugs in CRC studies. Our de novo CRC cell line, PUMC-CRC1 (Peking Union Medical College Colorectal Cancer 1) was derived from a 47-year-old Chinese female patient diagnosed with moderately to poorly differentiated colon adenocarcinoma. Multiple experiments were used for full characterization. The new cell line was epithelial-like and was passaged for more than 40 times, with a population doubling time of 44 h in vitro, detected by cell counts. The cells exhibited complicated chromosomal abnormalities. The tumor formation rate in SCID mice was 100%. The xenograft tumor was adenocarcinoma with poor to moderate differentiation by Haematoxylin and Eosin staining (H&E) sections. Immunohistochemistry (IHC) analysis and next-generation sequencing (NGS) revealed microsatellite stable (MSS), APC (p.T1493fs) inactivation, KRAS (p.G12V) activation, and SMAD4 (p.V506A) mutation. Quality control of the cell line proved mycoplasma negative and identical STR profile with that of the original tissue, and no interspecific or intraspecific cross contamination was detected. In conclusion, PUMC-CRC1 was a newly established and well characterized human colon cancer cell line, which might be a good model for both in vitro and in vivo studies of the mechanism of colon cancer progression and the treatment strategies for MSS CRC.

Genomic Landscape Alterations in Primary Tumor and Matched Lymph Node Metastasis in Hormone-Naïve Prostate Cancer Patients

“…Generally, cancer has been linked to mutations in the ERBB4 gene [ 25 ], which in this study was confirmed as a highly unstable gene. No information was found on the sequenced P.Q264* missense mutation in this gene, even if this has been already sequenced in a colon cancer cell line [ 26 ]. Regarding CSF1R , as for the SNP rs386693509, no MAF is reported in the databank for this mutation, but it has been sequenced on 15 patients out of 17 in our cohort (88.2%).…”

Overview of research progress and application of experimental models of colorectal cancer

“…The appropriate cell lines are selected based on the type of cancer or gene expression levels, according to the aims of the study. SW620, Caco-2, RKO, SW480, HT8, HT29, HT116, LoVo, and LS174 T cell lines are currently widely used in basic research studies on CRC ( Akashi et al, 2000 ; Vécsey et al, 2002 ; Lind et al, 2004 ; Barretina et al, 2012 ; Ahmed et al, 2013 ; Gemei et al, 2013; Mouradov et al, 2014 ; Maletzki et al, 2015 ; Boot et al, 2016 ; Berg et al, 2017 ; Mooi et al, 2018 ; Kim et al, 2020 ; Bian et al, 2021 ).…”

Patient-Derived Organoids of Colorectal Cancer: A Useful Tool for Personalized Medicine

“…Generally, primary culture of CRC cells involves isolating fresh tumor tissues via enzymatic treatment and culturing them in fetal bovine serum supplemented media [ 13 ]. Obtaining cell lines that can undergo stable passage is challenging; Bian et al mention that the success rate of cell line establishment from a fresh CRC tumor tissue is only approximately 10% [ 14 ]. Each 2D-cultured CRC cell line shows distinct morphological features.…”