Establishment and characterization of a bovine mammary epithelial cell line with unique properties

Zavizion, Boris; Duffelen, Marilyn van; Schaeffer, Warren I.; Politis, I.

doi:10.1007/bf02723679

Cited by 106 publications

(93 citation statements)

References 24 publications

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“…The effect of calcium on proliferation of BME-UV1 cells is an interesting phenomenon. This is because these cells have been produced from primary mammary epithelial cells by microinjection with a plasmid bearing the DNA sequence for SV-40 large T-antigen (Zavizion et al 1996). Therefore, the question is whether these cells have maintained the normal phenotype or have become neoplastic.…”

Section: Discussionmentioning

confidence: 99%

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The effect of calcium on mammary epithelial cell proliferation and the plasminogen activating system

Cheli

Zavizion²,

Todoulou³

et al. 1999

Can. J. Anim. Sci.

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Cheli, F., Zavizion, B., Todoulou, O. and Politis, I. 1999. The effect of calcium on mammary epithelial cell proliferation and the plasminogen activating system. Can. J. Anim. Sci. 79: 277-283. The objectives of this study were to examine 1) the effect of extracellular calcium on proliferation of bovine mammary epithelial cells, 2) whether extracellular calcium regulates the mitogenic effect of insulin-like growth factor (IGF-I) and epidermal growth factor (EGF) towards mammary epithelial cells, and 3) whether the effects of calcium on growth are mediated through changes in the plasminogen activating system. The BME-UV1 cells were used as a model system. Results showed that optimal proliferation of BME-UV1 cells grown in the presence of 10% dialyzed FBCS was achieved when the culture medium was supplemented with 1-2 mmol L -1 of extracellular Ca 2+ . IGF-I (P<0.01) but not EGF, increased proliferation of BME-UV1 cells. Furthermore, calcium does not regulate IGF-I and EGF responsiveness of BME-UV1 cells. Northern blot analysis was performed to examine the effect of extracellular calcium on expression of urokinasetype plasminogen activator (u-PA), PA inhibitor 1 (PAI-1) and u-PA receptor (u-PAR) genes by BME-UV1 cells in culture. Results showed that calcium increased expression of all above-mentioned genes after 24 h of exposure of cells to calcium, at a time that the effect of calcium on growth was not apparent. Calcium had no effect on u-PA and u-PAR expression after 48 and 72 h of exposure of cells to calcium, at a time that the effect of calcium on growth was predominant. Calcium caused a small increase of PAI-1 expression after 48 and 72 h but this small increase is apparently of limited biological value. Key words: Mammary epithelial cells, calcium, growth factors, plasminogen activating systemCheli, F., Zavizion, B., Todoulou, et Politis, I. 1999. Effet du calcium sur la prolifération des cellules de l'épithélium mammaire et sur le système activateur des plasminogènes. Can. J. Anim. Sci. 79: 277-283. L'objet de nos travaux était d'examiner 1) l'effet du calcium extracellulaire sur la prolifération des cellules épithéliales mammaires des bovins, 2) si le calcium extracellulaire module les effets mitogéniques de IGF-I et du facteur de croissance épidermique (FCE) vers les cellules épithéliales mammaires et 3) si les effets du calcium sur la croissance sont produits par la médiation de changements affectant le système activateur des plasminogènes. Les cellules de la souche de cellule épithéliale mammaire BME-UV1 étaient utilisées comme système modèle. Les résultats montrent que la prolifération optimale des cellules BME-UV1 mises en culture en présence d'une suspension à 10 % de sérum fétal bovin (FBCS) dialysé était réalisée lorsque le milieu de culture était complémenté par 1 à 2 mmol L -1 de Ca 2+ extracellulaire. IGF-I accroissait (P < 0,01) la prolifération des cellules BME-

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Section: Discussionmentioning

confidence: 99%

“…The origin of the BME-UV1 bovine mammary epithelial cell line was described previously (Zavizion et al 1996). Briefly, these cells were produced from primary mammary epithelial cells following microinjection with a plasmid carrying the sequence of the simian virus 40 early region mutant tsA58, encoding the thermolabile large T-antigen.…”

Section: Cell Culturementioning

confidence: 99%

The effect of calcium on mammary epithelial cell proliferation and the plasminogen activating system

Cheli

Zavizion²,

Todoulou³

et al. 1999

Can. J. Anim. Sci.

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“…Several ruminant immortalized mammary epithelial cell lines as MAC-T [15] and BME-UV [16] were generated by genomic integration of Simian virus large T-antigen (SV40LTA). However, transformed mammary cells are genetically and (usually) phenotypically changed.…”

Section: Mammary Tissue-derived Primary Cells As An In Vitro Modelmentioning

confidence: 99%

Development of an In Vitro Goat Mammary Gland Model: Establishment, Characterization, and Applications of Primary Goat Mammary Cell Cultures

Ogorevc¹,

Zorc²,

Dovč³

2018

Goat Science

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Alternatives to animal experiments, based on in vitro methodologies, have been suggested and adopted in the last decades in order to completely substitute or to reduce animal numbers in in vivo assays. In this chapter we describe methods for establishment, maintenance, and characterization of primary goat mammary epithelial cell cultures (pgMECs) and possible applications for which the derived primary cell model can be used instead of in vivo experiments. The established cell lines were grown in vitro for several passages and remained hormone and immune responsive and capable of milk protein synthesis. Knowledge on goat mammary cells and their manipulation is applicable to different fields of research; for example, it could be used in basic research to study mammary development and lactation biology, in agriculture to enhance lactation yield and persistency or to produce milk with special characteristics, in biopharma to express recombinant proteins in goat milk, or in biomedicine to study lactation, mammary development, and pathology, including neoplasia. The established cells represent an adequate surrogate for mammary gland; were successfully used to study mammary gland immunity, lactation, and mammary stem/progenitor cells; and have a potential to be used for other purposes.

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“…A number of bovine mammary cell lines have been established, among these the MAC-T (Huynh et al, 1991) and the BME-UV1 (Zavizion et al, 1996) cell lines, both of which were immortalised by transfection with the SV40 T-antigen. MAC-T cells have been used for studies of many bioactives, including fatty acids, growth factors, steroids, retinoids, cytokines and mammary extracts, and parameters such as viability, proliferation, apoptosis, gene expression, epithelial transport, cell signalling and lipogenesis (Woodward et al, 1996;Cohick and Turner, 1998;Berry et al, 2003;Peterson et al, 2004;Zarzynska et al, 2005;Thorn et al, 2006 andBruzelius et al, 2008;Keating et al, 2008;Sorensen et al, 2008;Zhou et al, 2008).…”

Section: Cell-based Models Of the Mammary Glandmentioning

confidence: 99%

Cell-based models to test the effects of milk-derived bioactives

Purup

Nielsen²

2012

Animal

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The life science industries have a strong interest in screening for novel bioactives in complex mixtures like milk and dairy products. Food bioactives are not only important for public health in general, but also have potential therapeutic applications for the treatment of a number of diseases. To identify these novel bioactives, establishment of robust screening assays is essential. The use of in vitro cell-based models for screening and testing have the advantage that several concentrations of mixtures or specific compounds can be assayed at the same time in cells from specific tissues. Primary cell cultures from target organs or established cell lines can be used to identify the most sensitive cells. In addition, a large number of transfected cell lines with very specific sensitivities have been developed. Different endpoints inherent to basal or more sophisticated cellular functions can be investigated, such as cell viability, apoptosis, migration, intracellular signalling, regulation of gene expression, morphology and metabolic alterations. The gastrointestinal tract is an obvious target for bioactive molecules delivered through milk and dairy products, because it lies at the interface between dietary components in the lumen and the internal processes of the host. Identification of bioactive factors that affects proliferation or migration of epithelial cells may have potential applications in promoting gastrointestinal health in both humans and animals. The mammary gland is another target organ of considerable interest since it has been estimated that up to 50% of all newly diagnosed breast cancers may be related to dietary factors. A large number of gastrointestinal and mammary epithelial cell lines are commercially available, but in order to study some cellular functions, primary cultures of freshly isolated cells are often preferred, as established cell lines do not always express specialised properties in culture.

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Establishment and characterization of a bovine mammary epithelial cell line with unique properties

Cited by 106 publications

References 24 publications

The effect of calcium on mammary epithelial cell proliferation and the plasminogen activating system

The effect of calcium on mammary epithelial cell proliferation and the plasminogen activating system

Development of an In Vitro Goat Mammary Gland Model: Establishment, Characterization, and Applications of Primary Goat Mammary Cell Cultures

Cell-based models to test the effects of milk-derived bioactives

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