Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility

Abstract

A cell line derived from the head kidney of golden pompano Trachinotus ovatus (TOHK) was established and characterized in this study. The TOHK cells grew most rapidly at 28° C and the optimum foetal bovine serum concentration in L-15 medium was 10%. The TOHK cells have a diploid chromosome number of 2N = 54. The transfection efficiency of TOHK cells was 7·5% at the 15th passage and 72% at the 40th passage. The transfection efficiency in TOHK cells was high, so these cells are suitable for foreign gene expression. The cytotoxic effects of heavy metals and extracellular products from Vibrio anguillarum and Vibrio alginolyticus were demonstrated in TOHK cells, so this TOHK cell line could also be applied in environmental monitoring of heavy metals and pathogenic bacteria. TOHK cell line showed high virus susceptibility, such as grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). Then, TOHK cell line could be used for the study of viral pathogenesis and the development of antiviral strategies.

Establishment of a new fish cell line from the caudal fin of golden pompano Trachinotus ovatus and its susceptibility to iridovirus

“…The TOCF cells had been cultured with L‐15 medium at 28° C for more than 100 passages. The culture condition was consistent with other established T. ovatus cell lines (Li et al, ; Yu et al, ; Li et al, ; Zhou et al, ).…”

“…Chromosomes are involved in the mechanism of inheritance and the expression of the genetic characteristics through successive generations (Jing et al, ). The chromosome numbers of TOCF cells counted at passage 30 ranged from 32 to 66 with the modal chromosome number found to be 54 (2 N = 54), which is in accordance with the chromosome count of GPS, TOGB, TOK and TOHK cells (Li et al, ; Yu et al, ; Li et al, ; Zhou et al, ). Moreover, the relative percentage of TOCF cells in the G 0 –G 1 , S and G 2 –M stages was determined using flow cytometry.…”

“…In this study, TOCF cells showed high susceptibility to SGIV, which was confirmed by the occurrence of CPE with the formation of inclusion bodies. In contrast, no CPE and viral replication were examined after RGNNV incubation, which is different from RGNNV‐sensitive TOGB cells, TOK and TOHK cells (Li et al, ; Li et al, ; Zhou et al, ). Moreover, the transcription levels of the MCP gene, increased significantly as the infection time increased.…”

“…The establishment of fish cell lines from host animals is crucial for the isolation, identification and characterization of pathogenic viruses from fish (Swaminathan et al, ). As essential models and tools, brain (TOGB), snout (GPS), mid‐kidney (TOK) and head kidney (TOHK) cell lines from T. ovatus have been applied for virus isolation (Li et al, ; Yu et al, ; Li et al, ; Zhou et al, ). However, characterization of T. ovatus cells derived from the caudal fin has not been reported.…”

First identification of the nervous necrosis virus isolated from cultured golden pompano (Trachinotus ovatus) in Guangxi, China

“…In severe outbreaks, NNV caused mortality in excess of 95% (Harikrishnan, Balasundaram, & Heo, ; Woo, Leatherland, & Bruno, ). As a commercially important cultured marine fish, golden pompano Trachinotus ovatus , Carangidae, Perciformes, is widespread across in China, Japan and some other Asian countries (Li et al., , ; Wang, Feng, Su, Ye, & Wang, ; Zhou, Ge, Lin, & Niu, ). Guangxi Province is an important cultured centre for tropical marine fish species in South China.…”