Establishment and characterization of a human gastric scirrhous carcinoma cell line in serum‐free chemically defined medium

Abstract

We have established a human gastric scirrhous carcinoma cell line (designated as HSC-43) in a serum-free chemically defined medium (CDM) without any polypeptide growth factor, from a primary tumor of a 56-year-old male patient. HSC-43 cells grew in vitro in adherence with a population doubling time of 55 hr, and had the cytological properties of mucinous epithelial tumor cells. Cytogenetic analysis of the cells revealed pseudotetraploidy, with structural abnormalities of deletion at chromosome Iq25 and with 3 marker chromosomes. Some cells had retained features of signet-ring cells and caused fibroblastic proliferation when transplanted into athymic nude mice. The possible involvement of transforming growth factor-alpha (TGF-alpha), and its receptor, the epidermal-growth-factor receptor (EGFR), on the growth of HSC-43 cells was studied. Synthesis and secretion of TGF-alpha by HSC-43 cells were confirmed by biological assay and enzyme-linked immunosorbent assay. Radioreceptor analysis showed the presence of receptors for EGF in HSC-43 cells. Proliferation of HSC-43 cells was inhibited by antibodies against TGF-alpha and/or the EGFR. However, neither TGF-alpha nor epidermal growth factor (EGF) was effective in stimulating the cell growth of HSC-43 cells, irrespective of the cell density when supplemented exogenously. Our data suggest that TGF-alpha and EGFR play a role in the autocrine growth of HSC-43 cells. This may be another example of growth regulation of gastric carcinoma.

Transforming growth factor-β decreases the cancer-initiating cell population within diffuse-type gastric carcinoma cells

“…Human diffuse-type gastric carcinoma cells were cultured as shown in Supplementary Table S1 (Takemura et al, 2004;Yashiro et al, 2009;Yanagihara et al, 1993). Lentivirus vectors were used to generate 2MLN-dnTbRII and control 2MLN-GFP cells as described .…”

Establishment and Characterization of Two Cell Lines from N‐Methyl‐N‐nitrosourea‐induced Mouse Glandular Stomach Carcinomas

“…A large bank of cultured cell lines reflecting this diversity of tumor phenotypes may thus be necessary to elucidate underlying mechanisms. Establishment of gastric epithelial cell lines, however, is limited by culturing difficulties and while some have been obtained from human stomach carcinomas, [1][2][3][4] most were derived from metastatic lesions in the peritoneal cavity, lymph node and liver and were relatively undifferentiated. Primary culture of fetal 5) and adult normal rat gastric epithelium has been used for functional studies of tissue-specific products, but no immortalized cell lines have been reported except for one from Nmethyl-N′-nitro-N-nitrosoguanidine-induced rat gastric carcinoma.…”

Establishment of two cell lines from human gastric scirrhous carcinoma that possess the potential to metastasize spontaneously in nude mice

“…The sources of the other stomach cancer cell lines established before have been described previously. [10][11][12] This study was conducted in accordance with the Declaration of Helsinki. Informed consent was obtained from all of the patients.…”

“…The morphology of the cells was similar to that of the signet-ring cell carcinoma cell lines described previously. [10][11][12] The HSC-57 line exhibited typical morphologic features of epithelial cells, characterized by sheets of polygonal cells in a pavement-like arrangement (Fig. 1E).…”

“…9) In order to address this problem, we previously established and characterized four cell lines from a primary gastric carcinoma and disseminated metastatic lesions of gastric scirrhous carcinoma. [10][11][12] These cell lines did not exhibit the potential to form experimental or spontaneous metastases when injected s.c. or i.v., or implanted orthotopically into nude mice.…”