Establishment and Characterization of an Oral Mucosal Melanoma Cell Line (MEMO) Derived From a Longstanding Primary Oral Melanoma

Lourenço, Sílvia Vanessa; Bologna, Sheyla Batista; Hsieh, Ricardo; Sangüeza, Martín; Fernandes, Juliana Dumêt; Nico, Marcello Menta Simonsen

doi:10.1097/dad.0b013e31826a9905

Cited by 11 publications

(15 citation statements)

References 16 publications

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“…Mucosal melanoma may also originate from melanocyte precursors residing in the submucosa as a result of arrest in their migration from the neural crest. 12,41 Immunoreactivity of melanoma cells to antibodies against S-100 protein, MART-1 (Melan-A) and gp100 (HMB-45) can be useful to distinguish oral melanoma from other malignancies. 42,43 Yu et al 43 examined the expression of HMB-45, S-100 and Melana-A in primary oral and nasal melanomas and their results indicate that HMB-45 and S-100 are positive in 94% and 88% of cases respectively.…”

Section: Dermoscopy Of An Amalgam Tattoo Dermoscopy Image Showing a mentioning

confidence: 99%

“…10,11 POMM are believed to arise from pigmented nevi, pre-existing pigmented areas or de novo (30% cases) from apparently normal mucosa. 9,12 Although Kahn et al 13 described transformation of a benign oral pigmentation to primary oral melanoma, a definite precursor lesion has not been identified. 14,15 In the oral cavity, mechanical trauma including injury from ill-fitting prostheses, infection, tobacco use has been cited as possible causative factors, but its etiological role seems unlikely.…”

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confidence: 99%

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Melanoma of the oral cavity: pathogenesis, dermoscopy, clinical features, staging and management

Warszawik-Hendzel

Słowińska²,

Olszewska

et al. 2014

J Dermatol Case Rep

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Primary mucosal melanoma of the oral cavity is an exceedingly rare neoplasm which is estimated to comprise 1-2% of all oral malignancies. In contrast to cutaneous melanomas, the risk factors and pathogenesis are poorly understood. The predominate localization of primary oral melanoma is hard palate and maxillary alveolus. Dermoscopy may be utilized as an adjunctive tool in the clinical differential diagnosis of oral mucosal melanoma whenever the lesion is accessible with a dermoscope. Surgery is the mainstay of treatment, but it may be challenging depending on the location of the tumor within the oral cavity and its size. Adjuvant therapy with dacarbazine, platinum analogs, nitrosoureas and interleukin-2 have been utilized with low response rates. Imatinib may be effective for patients with with c-Kit gene mutations. Sunitinib and dasatinib have been reported effective in selected cases. Vemurafenib and dabrafenib are targeted agents for patients with BRAF mutation-positive melanoma. Ipilimumab, an anti-cytotoxic T-lymphocyte antigen 4 antibody and pembrolizumab, a monoclonal antibody targeting programmed death 1 receptor may be a feasible treatment option in patients with metastatic mucosal melanoma. (J Dermatol Case Rep. 2014; 8(3) Melanoma is a malignant tumor that arises from melanocytes and is most commonly cutaneous in origin. Extracutaneous melanomas are known to be exceedingly rare and aggressive neoplasms that embrace ocular, mucosal and leptomeningeal melanomas. EpidemiologyMucosal melanomas are estimated to comprise 4-6.8 % of all primary melanomas. 1The incidence rate for mucosal melanoma (MM) is 2.3 per million. 1,2The incidence of MM increases with age. Older patients have tenfold higher incidence of MM compared to patients under the age of 60 years. 2Primary oral mucosal melanoma (POMM) is excessively uncommon in prepubertal children. 3Although there is a slight male preponderance (1.2:1), 1,4 some studies have been reported higher gender distribution of mucosal melanoma for women, 5,6 other indicate that there is no significant difference between sexes in the incidence of this tumor. 2,7The incidence rate of POMM is highest among Asian men. 3,6 Among the Japanese oral melanoma accounts for 7.5 % of all melanomas versus less than 1 % in Caucasians. 8,9Pathogenesis In contrast to cutaneous melanomas, the etiology and pathogenesis of POMM is poorly understood and no etiological and intraoral risk factors, other than preexisting pigmented nevi, have been identified. 10,11 POMM are believed to arise from pigmented nevi, pre-existing pigmented areas or de novo (30% cases) from apparently normal mucosa. 9,12 Although Kahn et al 13 described transformation of a benign oral pigmentation to primary oral melanoma, a definite precursor lesion has not been identified. 14,15 In the oral cavity, mechanical trauma including injury from ill-fitting prostheses, infection, tobacco use has been cited as possible causative factors, but its etiological role seems unlikely. 30Amelanotic oral melanoma is partic...

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Section: Dermoscopy Of An Amalgam Tattoo Dermoscopy Image Showing a mentioning

confidence: 99%

mentioning

confidence: 99%

Melanoma of the oral cavity: pathogenesis, dermoscopy, clinical features, staging and management

Warszawik-Hendzel

Słowińska²,

Olszewska

et al. 2014

J Dermatol Case Rep

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“…Oral mucosal melanomas account for between 0.26 and 0.5 % of all oral malignancies [8,9], and for unknown reasons their incidence and prevalence are higher in Asia than in North America or Europe [9]. Their incidence and prevalence in Africa are unknown.…”

Section: Introductionmentioning

confidence: 99%

“…The palate and the maxillary gingiva of elderly men are most frequently affected [8,[11][12][13][14][15]. Oral mucosal melanomas are usually painless and grow rapidly, so they are often diagnosed late in the course of the disease when the lesions are already large and have metastasized to regional lymph nodes.…”

Section: Introductionmentioning

confidence: 99%

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Oral Mucosal Melanoma: Some Pathobiological Considerations and an Illustrative Report of a Case

Tlholoe

Khammissa

Bouckaert

et al. 2014

Head and Neck Pathol

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Oral mucosal melanoma is a relatively rare malignancy with an aggressive clinico-pathological behaviour. The mean 5-year survival rate is about 15 %. It arises primarily from melanocytes found in the basal cell layer of the epithelium, but may sometimes arise from melanocytes residing in the lamina propria. The pathogenesis is complex, and few of the molecular mechanisms underlying the development of oral mucosal melanoma have been defined. The extraneous risk factors associated with oral mucosal melanoma, if any, are unknown. Oral mucosal melanomas account for about 25 % of all mucosal melanomas of the head and neck, and exhibit a profile of cytogenetic alterations, and a pathobiological behaviour and clinical course different from that of cutaneous melanomas. As they are usually painless and grow quickly, as a rule, they are diagnosed late in the course of the disease when the lesions are already large and have metastasized to regional lymph nodes. In this paper we discuss some aspects of the pathobiology of oral mucosal melanoma, and present an illustrative case report.

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Identification of mutations in canine oral mucosal melanomas by exome sequencing and comparison with human melanomas

Dagli,

Nagamine,

Ikeda

et al. 2024

Sci Rep

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Oral mucosal melanomas (OMMs) are aggressive neoplasms commonly found in dogs but rare in humans. Utilizing whole exome sequencing (WES), which focuses on protein-coding regions to reveal mutation profiles, we conducted a comparative analysis of canine OMM and human melanomas. This study involved DNA extraction, exome enrichment, and sequencing from three canine OMM cell lines (CMGD-2, CMGD-5, TLM-1), five canine OMM frozen samples, a human OMM cell line (MEMO), and a human commercial skin melanoma cell line (SK-MEL-28). The sequencing and subsequent analysis of FASTQ files yielded final variant files, leading to the identification of mutations. Our findings revealed a total of 500 mutated genes in canine OMM, including significant ones such as EP300, FAT4, JAK3, LRP1B, NCOR1, and NOTCH1. Notably, 82 shared mutations were identified between human melanomas and canine OMM genomes. These mutations were categorized based on the gene functions. The identification of these mutations provides critical insights that can pave the way for the development of novel therapeutic strategies for both canine and human OMM, offering hope for more effective treatments in the future. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-74748-z.

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Establishment and Characterization of an Oral Mucosal Melanoma Cell Line (MEMO) Derived From a Longstanding Primary Oral Melanoma

Cited by 11 publications

References 16 publications

Melanoma of the oral cavity: pathogenesis, dermoscopy, clinical features, staging and management

Melanoma of the oral cavity: pathogenesis, dermoscopy, clinical features, staging and management

Oral Mucosal Melanoma: Some Pathobiological Considerations and an Illustrative Report of a Case

Identification of mutations in canine oral mucosal melanomas by exome sequencing and comparison with human melanomas

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