Establishment and Characterization of Colon Carcinoma and Renal Cell Carcinoma Primary Cultures

Abstract

Patients with metastatic renal and colon carcinoma have a very poor prognosis. In many cases, the tumor recurs after surgical excision and chemotherapy. Therefore, it might be beneficial for cancer patients to induce an immune attack against the tumor by inserting a cytokine gene into the tumor cells. Here, two different techniques for isolation of single tumor cells were compared. An enzymatic solution was superior to an EDTA/DTT isolation solution for establishing tumor primary cultures. In total, 18 primary cell cultures could be established from 68 patients with colon and renal cell carcinoma. Cells were further characterized concerning fibroblast contamination, cell proliferation and HLA-typing. These primary tumor cells might be of value for cytokine gene transfer and in vaccination protocols for cancer patients.

Primary Cell Cultures Arising from Normal Kidney and Renal Cell Carcinoma Retain the Proteomic Profile of Corresponding Tissues

“…In fact, the plated tissues were removed from dishes after 5 days when a number of epithelial cells had spread out but the fibroblasts, that can easily be distinguished from renal epithelial cells, had not yet started to massively grow out from the frustules. In this manner, fibroblast overgrowth was not a significant problem as in agreement with others. ,, The same stands true for endothelial cells that require specific culture conditions in order to proliferate. The cytological homogeneity of our primary cultures was evaluated by immunocytochemical stains at passage 3, and in all instances the reactions were homogeneous in intensity and had identical profiles.…”

Primary Cell Cultures from Human Renal Cortex and Renal-Cell Carcinoma Evidence a Differential Expression of Two Spliced Isoforms of Annexin A3

“…Tissues, normal or neoplastic, were washed two times in PBS pH 7.2 at 4°C and minced in 1 mm 3 fragments in a Petri dish. The small fragments were then incubated for 2 hours at 37°C with DMEM-F12 and 1.25 mg/ml Collage- 15 Cell morphology was observed in contrast phase using an Inverted Olympus CK40 microscope at ϫ40 magnification.…”

Hippo Signaling Mediates Proliferation, Invasiveness, and Metastatic Potential of Clear Cell Renal Cell Carcinoma

“…MZ1774, B1, B3, and RCC177 are primary RCC cell lines and have been described in [8], [9], [10]. The human RCC cell line ACHN (ATCC CRL-1611) was maintained in Dulbecco's modified Eagle's medium (PAA Laboratories) supplemented with 10% FBS, 1 × penicillin/ streptomycin (both PAA Laboratories), and 5 μg/ml plasmocin (InvivoGen).…”