Establishment and characterization of highly osteolytic luminal breast cancer cell lines by intracaudal arterial injection

Han, Yuxuan; Nakayama, Jun; Hayashi, Yusuke; Jeong, Seongmoon; Futakuchi, Mitsuru; Ito, Emi; Watanabe, Shinya; Semba, Kentaro

doi:10.1111/gtc.12743

Cited by 29 publications

(42 citation statements)

References 38 publications

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“…C arietinum sprouts are a very rich source of isoflavones and could be used to supply dietary phytoestrogens together with soybeans [ 18 ]. The time and intensity of UVB-light exposure optimal for sprout growth and isoflavone content were determined in previous research [ 16 , 20 ]. The lactic acid fermentation of sprouts with Lactobacillus casei 0979 strain and β-glucosidase treatment was performed to increase the isoflavone content and increase the aglycone forms [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…Bone tissue is one of the most common metastatic sites for breast cancer [ 20 ]; the effect of C. arietinum isoflavones on the detachment and migration of breast-cancer MCF-7 cells was assessed. As can be seen in Figure 10 A–C, all the samples decreased the migration ratio of MCF-7 cells, as well as the scratch-area reduction: ChU had the least effect, whereas the highest activity was observed for ChUFH.…”

Section: Resultsmentioning

confidence: 99%

“…The increase in cell growth and proliferation may lead to hypoxia, which, in turn, elevates the generation of reactive oxygen species and metastasis [ 20 ]. As demonstrated in Figure 11 , the pre-incubation of the MCF-7 cells with the extracts at IC 0 doses diminished the intracellular ROS levels by 10–15% compared to those in the control cells.…”

Section: Resultsmentioning

confidence: 99%

“…MCF-7 human breast-cancer cells are known to express both isoforms of estrogen receptors (ERα and ERβ) [ 29 ], and were thus used in a proliferation experiment to evaluate the estrogenic potency of C. arietinum extracts. Taking into account that bone is one of the most common metastatic sites for breast cancer [ 20 ], the effect of the C. arietinum isoflavones on MCF-7 cell migration was assessed. The C. arietinum sprout preparations were able to decrease migration without cell detachment.…”

Section: Discussionmentioning

confidence: 99%

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Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

et al. 2020

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In the present study, we investigated the biological activity of four extracts obtained from Cicer arietinum L. sprouts. The fermentation of the sprouts with Lactobacillus casei and their incubation with β-glucosidase elevated the concentrations of isoflavonoids, especially coumestrol, formononetin and biochanin A. To study the biological activity of C. arietinum, the human osteosarcoma Saos-2 and human breast cancer MCF-7 cell lines were used. The extracts obtained from fermented sprouts exhibited the strongest ability to decrease intracellular oxidative stress in both types of cells. They augmented mineralization and alkaline phosphatase activity in Saos-2 cells, as well as diminished the secretion of interleukin-6 and tumor necrosis factor α. Simultaneously, the extracts, at the same doses, inhibited the migration of MCF-7 cells. On the other hand, elevated concentrations of C. arietinum induced apoptosis in estrogen-dependent MCF-7 cells, while lower doses stimulated cell proliferation. These results are important for carefully considering the use of fermented C. arietinum sprouts as a dietary supplement component for the prevention of osteoporosis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

et al. 2020

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“…Survival analysis was conducted for breast cancer patients in the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) datasets as previously described [26,79]. We used a cohort of HER2-positive breast cancer patients and all breast cancer patients for this survival analysis.…”

Section: Survival Analysismentioning

confidence: 99%

Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines

Kuroiwa

Nakayama

Adachi

et al. 2020

Cancers

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HER2 is overexpressed in 25–30% of breast cancers, and approximately 30% of HER2-positive breast cancers metastasize to the brain. Although the incidence of brain metastasis in HER2-positive breast cancer is high, previous studies have been mainly based on cell lines of the triple-negative subtype, and the molecular mechanisms of brain metastasis in HER2-positive breast cancer are unclear. In the present study, we performed intracranial injection using nine HER2-positive breast cancer cell lines to evaluate their proliferative activity in brain tissue. Our results show that UACC-893 and MDA-MB-453 cells rapidly proliferated in the brain parenchyma, while the other seven cell lines moderately or slowly proliferated. Among these nine cell lines, the proliferative activity in brain tissue was not correlated with either the HER2 level or the HER2 phosphorylation status. To extract signature genes associated with brain colonization, we conducted microarray analysis and found that these two cell lines shared 138 gene expression patterns. Moreover, some of these genes were correlated with poor prognosis in HER2-positive breast cancer patients. Our findings might be helpful for further studying brain metastasis in HER2-positive breast cancer.

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Osteoblast‐derived extracellular vesicles exert osteoblastic and tumor‐suppressive functions via SERPINA3 and LCN2 in prostate cancer

Ito

Hayashi²,

Sato

et al. 2023

Molecular Oncology

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Clinically, the osteolytic phenotype is rare in prostate cancer (PCa), and the prognosis is generally worse than that of the osteoblastic phenotype. Osteoblastic prostate cancer (BPCa) is a major type of bone metastasis. Several factors responsible for osteogenesis have been identified, but the molecular mechanism of osteoblastic bone metastasis in PCa is not fully understood. Here, we show the osteogenic and tumor‐suppressive roles of SERPINA3 and LCN2 in BPCa. In a co‐culture of osteoblasts (OBs) and BPCa cells, SERPINA3 and LCN2 were remarkably upregulated in BPCa via OB‐derived extracellular vesicles, while they were not in the co‐culture of OBs and osteolytic prostate cancer (LPCa) cells. In both the co‐culture system and mouse xenograft experiments with intracaudal injection, enhanced expression of SERPINA3 and LCN2 in PCa led to osteogenesis. Additionally, the addition of SERPINA3 and LCN2 to BPCa cells significantly suppressed the proliferative potential. Retrospective analysis also confirmed that high expression levels of SERPINA3 and LCN2 were significantly correlated with a better prognosis. Our results may partially explain how osteoblastic bone metastasis develops and why the prognosis for BPCa is relatively better than that for LPCa.

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Establishment and characterization of highly osteolytic luminal breast cancer cell lines by intracaudal arterial injection

Cited by 29 publications

References 38 publications

Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines

Osteoblast‐derived extracellular vesicles exert osteoblastic and tumor‐suppressive functions via SERPINA3 and LCN2 in prostate cancer

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