Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha

Sakai, Osamu; Tatsuhito,; Uchida, Katsuhisa; Igase, Masaya; Mizuno, Takuya

doi:10.1089/mab.2020.0002

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…In the Ventana OptiView DAB Universal Kit (Ventana Medical Systems), sections were incubated with Linker-HQ at 37 °C for 8 minutes, and then incubated with Multimer-HRP at 37 °C for 8 minutes. To detect dog IgM, IgG, IgA, or CD8α, 30 sections were incubated with biotin-conjugated anti-goat immunoglobulin G (1:400; KPL) or anti-rat immunoglobulin G (1:500; Dako) at 37 °C for 40 minutes, and then with horseradish peroxidase–conjugated streptavidin (1:400; Dako) at 37 °C for 40 minutes. Labeled complexes were visualized with 3,3′-diaminobenzidine (DAB) chromogen and 0.03% hydrogen peroxidase in Tris-HCl buffer and then counterstained with hematoxylin.…”

Section: Methodsmentioning

confidence: 99%

Histopathological features and immunophenotyping of canine transmural gastrointestinal lymphoma using full-thickness biopsy samples

et al. 2021

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To elucidate the histopathological characteristics and immunophenotypes of canine transmural “mass-forming” gastrointestinal lymphomas and plasmacytomas, 83 surgically resected biopsy samples were examined. All lymphomas and plasmacytomas were located in the small or large intestine except for 1 plasmacytoma which was in the stomach. According to the World Health Organization (WHO) classification, B-cell neoplasms (17 cases) included lymphoplasmacytic lymphoma (6/17), plasmacytoma (5/17), follicular lymphoma (3/17), and diffuse large B-cell lymphoma (3/17). Based on nuclear sizes, T-cell neoplasms (66 cases) were broadly divided into large cell lymphoma (LCL; 48/66) and small cell lymphoma (SCL; 18/66). According to the WHO classification, T-cell neoplasms included anaplastic large T-cell lymphoma (ALCL; 10/66), angiotropic T-cell lymphoma (3/66), mixed inflammatory type peripheral T-cell lymphoma (mixed inflammatory type PTCL; 33/66), and PTCL-not otherwise specified (PTCL-NOS; 20/66). Mixed inflammatory type PTCLs were further divided into histiocyte- (27/33) and eosinophil- (6/33) dominant types. Immunohistochemically, lymphoplasmacytic lymphomas were positive for Pax5 (6/6) and IgM (5/6), while plasmacytomas were positive for IgG (5/6) and negative for Pax5. LCLs were immunopositive for granzyme B in 31/48 cases (65%) and CD8 in 9/48 cases (19%), while SCLs were immunopositive for granzyme B in 3/18 cases (17%) and CD8 in 3/18 cases (17%). Furthermore, 8/10 cases (80%) of ALCL and 19/27 cases (70%) of histiocyte-dominant PTCL were immunopositive for granzyme B, whereas 6/20 cases (30%) of PTCL-NOS, 1/6 cases (17%) of eosinophil-dominant PTCL, and no cases of angiotropic T-cell lymphomas were immunopositive for granzyme B. The present study describes the immunophenotypes in different histological types of transmural gastrointestinal lymphomas in the dog.

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Section: Methodsmentioning

confidence: 99%

Histopathological features and immunophenotyping of canine transmural gastrointestinal lymphoma using full-thickness biopsy samples

et al. 2021

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“…The reactivities of CD8, granzyme B, CD30, CD56, and FOXP3 were confirmed in the following articles. 4,21,27,32,39 Sequence alignment of human and feline TIA1 was obtained from The Universal Protein Resource (UniProt) (Supplemental Fig. S1).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Intraepithelial cytotoxic lymphocytes are associated with a poor prognosis in feline intestinal T-cell lymphoma

Chambers

Nakashima

et al. 2022

Vet Pathol

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The expression of cytotoxic molecules in feline intestinal T-cell lymphoma cells was examined immunohistochemically using endoscopic samples of 50 cases. Cases included 14 large-cell lymphomas (LCLs) and 36 small-cell lymphomas (SCLs). Most LCL and some SCL exhibited marked erosion and villous atrophy. Clonal T-cell receptor (TCR) gene rearrangement was detected in 10/14 (71%) LCL cases and 33/36 (92%) SCL cases. No clonal immunoglobulin heavy chain (IgH) gene rearrangement was detected. Immunohistochemically, all cases were positive for CD3 and negative for CD79α, CD30, CD56, and Foxp3. LCLs were positive for CD8 in 13/14 cases (93%), T-cell intracellular antigen 1 (TIA1) in 14/14 cases (100%), and granzyme B in 6/14 cases (43%). SCLs were positive for CD8 in 28/36 cases (78%), TIA1 in 33/36 cases (92%), and granzyme B in 2/36 cases (6%). TIA1- and granzyme B-positive neoplastic lymphocytes were predominantly observed in the mucosal epithelium of 10/50 cases (20%) and 6/50 cases (12%), respectively. No significant differences in survival time were found based on cell size or epitheliotropism. However, cases with TIA1+ and/or granzyme B+ neoplastic lymphocytes predominantly in the mucosal epithelium had significantly shorter survival times ( P < .05), suggesting that mucosal epithelium infiltration of neoplastic cells with a cytotoxic immunophenotype is a negative prognostic factor. Therefore, intraepithelial cytotoxic lymphocytes may be associated with mucosal injury and impaired intestinal function, leading to a poor prognosis in cats with intestinal T-cell lymphoma.

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“…In the Ventana OptiView DAB Universal Kit (Ventana Medical Systems, Tucson, USA), sections were incubated with Linker-HQ at 37°C for 8 minutes and then incubated with Multimer-HRP at 37°C for 8 minutes. To detect CD8, 17 sections were incubated with biotin-conjugated anti-rat immunoglobulin G (1:500; Dako) at 37°C for 40 minutes and then with horseradish peroxidase-conjugated streptavidin (1:400; Dako) at 37°C for 40 minutes. Labeled complexes were visualized with 3,3' -diaminobenzidine chromogen and 0.03% hydrogen peroxide in Abbreviations: CD, cluster of differentiation; HLA-DR, human leukocyte antigen-DR; RTU, ready to use; HIER, heat-induced epitope retrieval.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Nodal T-zone lymphoma and T-zone hyperplasia in dogs

et al. 2022

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T-zone lymphoma (TZL) is an indolent, nodal lymphoma that has been clinically characterized in detail in dogs, and T-zone hyperplasia (TZH) is a hyperplastic change in lymph nodes associated with antigen processing. In some cases, histopathological features of TZL and TZH are similar, and are difficult to differentiate by morphology alone. Since there have been few publications characterizing their immunohistochemical profiles, histological, immunohistochemical, and clonality examinations were performed using formalin-fixed paraffin-embedded samples of canine lymph nodes with TZL (14 cases) and canine lymph nodes with TZH associated with nonlymphocytic tumors (10 cases). Immunohistochemically, small- to medium-sized lymphocytes of TZL were immunopositive for CD3, CD5, and HLA-DR, and negative for CD45, FOXP3, and granzyme B (GRB) in all cases. Among these 14 cases, 11 were immunopositive for CD8 and 1 was CD20 positive. Paracortical lymphocytes in TZH were diffusely immunopositive for CD3, CD5, and CD45, with scattered immunopositivity for CD8, HLA-DR, FOXP3, and GRB, and negative for CD20 in all cases. A clonal TCR gene rearrangement was detected in 13/14 TZL and none of the TZH cases. The present study revealed that TZL is a clonal proliferation of monomorphic CD8+CD45-GRB- T cells, while TZH consists of an immunophenotypically heterogenous population of CD45+ T cells that are variably positive for CD8 and FOXP3. These results suggest that canine TZL is a clonal proliferation of naïve or premature cytotoxic T cells. Regarding TZH, variable immunopositivity for cytotoxic and regulatory T-cell antigens may reflect immune responses to a variety of regional neoplastic lesions.

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Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha

Cited by 6 publications

References 19 publications

Histopathological features and immunophenotyping of canine transmural gastrointestinal lymphoma using full-thickness biopsy samples

Histopathological features and immunophenotyping of canine transmural gastrointestinal lymphoma using full-thickness biopsy samples

Intraepithelial cytotoxic lymphocytes are associated with a poor prognosis in feline intestinal T-cell lymphoma

Nodal T-zone lymphoma and T-zone hyperplasia in dogs

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