Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture: a biotechnology approach for mesquite gum production

Abstract

This study presents a protocol for the establishment of Prosopis laevigata cell suspension culture as a strategy to obtain an in vitro mesquite gum productive cell line. The callus used for this purpose was obtained with hypocotyls from 15-day-old plantlets, placed on Murashige-Skoog medium with two different plant growth regulators (PGRs), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T; 5.0 lM) and kinetin (KIN; 5.0 lM). With this PGRs treatment, after four subcultures (30 days each) an exuded gum-like substance was observed on the callus surface. The growth kinetics of the cell suspension culture showed a specific cell growth rate (l) of 0.14 d -1 and doubling time (t d ) of 6.6 days, respectively. The gum-like substance from callus culture and the broth from cell suspension culture were subjected to chemical analysis and compared with the mesquite gum exuded from wild trees. Both, gum-like substance from callus culture and the broth from cell suspension culture showed the presence of Arabinogalactanproteins, and their polysaccharide fraction presented the same monosaccharides as those isolated from mesquite gum. In addition, the emulsifying properties of gum-like substance from callus culture and the broth from cell suspension culture were compared to those of mesquite gum and all three samples exhibited similar emulsifying capacity and emulsification stability.

Establishment of a Cell Suspension Culture of Eysenhardtia platycarpa: Phytochemical Screening of Extracts and Evaluation of Antifungal Activity

“…In other Fabaceae species such as Prosopis laevigata , the in vitro germination can occur after 3–7 days in mechanically scarified seeds [ 14 , 15 ]. Regarding callus induction, a suitable response of callus formation was exhibited on internodal segments after 15 days of culture ( Figure 1 B).…”

“…It is known from callus induction treatments on semisolid culture medium that these do not always adapt best to a liquid culture medium. For instance, P. laevigata leaf explants presented 100% of callus with trichlorophenoxyacetic acid (2,4,5-T, 1.28 mg/L) with BAP (1.13 mg/L) or 2,4,5-T (1.28 mg/L) with KIN (1.08 mg/L); however, only calluses containing KIN were suitable for establishing the CSC [ 14 ]. This indicates that, even among closely related species, the genotype is an influential factor in the response of cell cultures; moreover, the PGRs activity varies depending on the presence of transporter or receptor biosynthesized proteins in the explants, affecting in vitro culture development [ 25 ].…”

Tolerancia y capacidad de remoción de Cr(VI) y Pb(II) en cultivos de células en suspensión de Prosopis laevigata

“…The duplication time (t d ) was 14.1 days, and specific growth rate (µ) was 0.049 days −1 . However, studies conducted by Trejo-Espino et al (2011) in a Prosopis leavigata cell suspension culture reported a lower MAB (11.9 g DW L −1 ). Instead the t d (6.6 days) and µ (0.104 days −1 ) were higher than those found in this study.…”

“…La combinación de 1.5 mg L -1 de 2,4-D con 1.0 mg L -1 KIN (D1.5K1.0) o con BAP (D1.5B1.0) presentaron significativamente el mayor porcentaje de inducción de callo (100%) así como las mejores características de friabilidad y rápido crecimiento (Tabla 6). En los reportes existentes para la inducción de callo en especies relacionadas, P. juliflora (Nandwani y Ramawat, 1991), P. tamarugo (Nandwani y Ramawat, 1991) y P. laevigata (Trejo-Espino et al, 2011), ha sido necesario el uso de la combinación de auxinas y citocininas para la obtención de callo. La inducción de callo se vio significativamente afectada por el tipo de explante (cotiledón, hipocótilo y raíz).…”

“…This yields a straight line over the exponential phase growth. The slope of the linear part of the plot corresponds to specific cell growth rate and is given in 1 per units of time (Trejo-Espino et al, 2011)).…”