Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines

Abstract

Permanently growing cell lines can be invaluable because of their usefulness in a variety of experimental situations. We report the characteristics of seven cell lines designated, SNU-306, SNU-334, SNU-1528, SNU-1553, SNU-1581, SNU-1958 and SNU-2372, which were established from three primary carcinomas, two pleural effusion, one pericardial effusion and one ascitic fluid samples obtained from seven Korean breast carcinoma patients. The histopathology of the primary tumors and their in vitro growth characteristics are described. DNA fingerprinting analysis and genetic alterations in the p53 and EGFR genes were conducted. The expression levels of the ER-α, PR, C-erbB2, E-cadherin, COX-2, MDR and MXR genes were investigated and sensitivity to anticancer drugs was screened. Growth was as adherent cells (four cell lines), floating aggregates (one cell line) and both (two cell lines). All lines were free of mycoplasma or bacteria and were proven unique by DNA fingerprinting analysis using 18 microsatellite markers. Estrogen receptor (ER) mRNA was highly expressed in five cell lines and low or undetectable in SNU-1958 and SNU-2372. Progesterone receptor (PR) mRNA was expressed only in the SNU-306. SNU-1958 and SNU-2372 were hormone receptor-negative and C-erbB2-negative (triple-negative). SNU-1528 had an in-frame deletion of 42 base pairs of p53 gene and showed over 20-fold resistance for taxol compared to the other cell lines. There were no mutation in the EGFR gene; COX-2 was expressed in four cell lines and MXR was expressed in two cell lines. These well-characterized seven breast cancer cell lines, which include two triple-negative cell lines, will be useful for the study of breast cancer biology.

Establishment and characterization of three stable Basal/HER2-positive breast cancer cell lines derived from Chinese breast carcinoma with identical missense mutations in the DNA-binding domain of TP53

“…Many studies have described methods for establishing breast cancer cell lines, including differential trypsinization [ 12 – 16 ], adherence [ 17 ], tissue inoculation [ 12 , 18 – 20 ], geneticin treatment [ 21 , 22 ], and differential centrifugation [ 23 ], among others. We used the cell scraping method.…”

Coxsackievirus B Type 4 Infection in β Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing

“…55 In addition, ERa reportedly forms an inactive cytoplasmic complex with URI and HSP90, and URI deletion promotes the nuclear translocation that ERa requires for transcriptional activity. 29 We detected endogenous interaction between URI and ERa in INS-1E cells, MCF-7 breast cancer cells (known to have high levels of ERa 56 ) and Pdx1-cre pancreases in co-immunoprecipitation (coIP) experiments, but not in Uri1 flox/flox ; Pdx1-cre pancreases (Figures 5G, S5J, and S5K), indicating its specificity. As mentioned, translocation to the nucleus is required for the transcriptional activity of ERa, and URI has been shown to sequester it in the cytoplasm.…”

Establishment and characterization of patient-derived xenograft models of gastrointestinal stromal tumor resistant to standard tyrosine kinase inhibitors

“…PCR was used to amplify 15 tetranucleotide repeat loci and sex-determining markers at loci containing highly polymorphic microsatellite markers. The amplified products were analyzed using an ABI 3730 genetic analyzer (Applied Biosystems, Foster City, CA, USA) [ 27 , 28 ].…”