Establishment and Characterization of Two Neoplastic Cell Lines (U‐1285 and U‐1568) Derived From Small Cell Carcinoma of the Lung

Abstract

Two human cell lines have been established in vitro from patients with small cell carcinoma of the lung (SCC). The U‐1285 line was derived from the classical small cell type of SCC while the U‐1568 orginated from a larger cell variant. The cell lines grow as suspension cultures and have been passaged continuously in vitro for 3 years (U‐1285) and 2 years (U‐1568), respectively. The malignant nature of the lines is suggested by their infinite growth potential, their capacity to form colonies in agarose and their aneuploidy. Both cell lines contain cytoplasmic electron dense particles indistinguishable from classical neurosecretory granules but only in U‐1568 has hormone production (human chorion gonadotropin (α‐HCG)) been proven. The U‐1568 line produces carcino‐embryonic antigen (CEA) while both U‐1285 and U‐1568 produce alpha‐feto‐protein (AFP). Chromosome analysis reveal aneuploidy of both lines. Among structural aberrations, involvement of chromosome 14 in U‐1285 and chromosomes 1, 7 and 12 in U‐1568 is interesting since alterations of these chromosomes have been described previously in other malignant conditions.

miRNA-214 modulates radiotherapy response of non-small cell lung cancer cells through regulation of p38MAPK, apoptosis and senescence

“…Total profiling of miRNA in different NSCLC and SCLC cell lines revealed that each cell line expressed ∼100–200 different miRNAs (Figure 1B). For each LC subtype, SCLC and NSCLC, the cell lines were grouped into RS (SF2<0.3) and RR (SF2⩾0.3) on the basis of their clonogenic survival data (Bergh et al , 1982, 1985; Carney et al , 1983; Carmichael et al , 1989; Sirzen et al , 1998). The expression of the different individual miRNAs within each of these cohorts was examined.…”

“…The human NSCLC cell lines H23, A549, H157, H661, H1299 and U-1810 and the SCLC cell lines H69, H82, U-1285, U-1690 and U-1906 were used (Bergh et al , 1982, 1985; Carney et al , 1983; Carmichael et al , 1989; Sirzen et al , 1998). All cells were maintained in RPMI 1640 medium (Invitrogen, Stockholm, Sweden) supplemented with 10% of heat-inactivated fetal bovine serum (FBS), ℒ-glutamine (2 mℳ), penicillin (100 U ml −1 ) and streptomycin (100  μ g ml −1 ) (Gibco, Invitrogen, Paisley, UK) at 37 °C and 5% CO 2 .…”

Arsenic trioxide is highly cytotoxic to small cell lung carcinoma cells

“…The fact that cells of As 2 O 3 nonresponding SCLC tumors were larger and that the SCLC-mv cell line (derived from a large cell variant of SCLC; ref. 35) was less sensitive to As 2 O 3 treatment may indicate that the highest efficacy of As 2 O 3 is to be expected in patients with classic small cell type SCLC.…”

Marine Sponge Cribrochalina vasculum Compounds Activate Intrinsic Apoptotic Signaling and Inhibit Growth Factor Signaling Cascades in Non–Small Cell Lung Carcinoma

“…The human SCLC U-1285 (20) and NSCLC U-1810 (21) were kind gifts from Uppsala University where they were established and characterized (22). SCLC H69 and H82, ovarian cancer cell lines A2780 and SKOV-3, foreskin fibroblasts immortalized with hTERT BJ-5ta, bronchial epithelial cells BEAS-2B and hTERT immortalized retina epithelial cells RPE-1 were all purchased from the ATCC.…”