Establishment and characterization of Xenopus oviduct cells in primary culture

Marsh, Joan; Tata, Jamshed R.

doi:10.1016/0014-4827(87)90337-5

Cited by 13 publications

(1 citation statement)

References 16 publications

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“…For example, adult Xenopus liver cells contain estrogen receptor and respond to estradiol by producing vitellogenin (for a review, see reference 29), but other cells which also contain receptor do not. Neither adult oviduct (22) nor even liver from premetamorphic tadpoles (11,13) does so, although both contain receptor and indeed respond to estradiol in other ways (17)(18)(19). Hence, it is clear that a certain level of receptor is sufficient to allow activation of chromosomal vitellogenin genes in adult liver cells, but it is equally clear that the presence of a similar amount in other cell types is not sufficient, even if the other cell types are inherently estrogen responsive.…”

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confidence: 92%

Activation of chromosomal vitellogenin genes in Xenopus oocytes by pure estrogen receptor and independent activation of albumin genes.

McKenzie¹,

Cridland²,

Knowland³

1990

Mol. Cell. Biol.

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We generated pure estrogen receptor protein in Xenopus oocytes by injecting them with estrogen receptor mRNA synthesized in vitro. A chromosomal vitellogenin gene, which normally responds to estrogen only in liver cells, is activated. Primer extension shows that initiation is accurate, and ribonuclease mapping shows that the first exon is correctly spliced out of the initial transcript. Long transcripts are produced, one

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