Establishment and Growth of Embryogenic Suspension Cultures of Ocotea catharinensis Mez. (Lauraceae)

Moser, Juliana Righetto; Garcia, Magali Gonçalves; Viana, Ana Maria

doi:10.1023/b:ticu.0000020387.96568.25

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…1b). This acidification was previously reported for cell suspension cultures of Rollinia mucosa (Figueiredo et al 2000) and Ocotea catharinensis (Moser et al 2004) and seems to be related to the uptake of ammonium due to cell growth.…”

Section: Resultsmentioning

confidence: 58%

Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

Júnior

Cavalari

Carpita

et al. 2010

Trees

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Rudgea jasminoides (Rubiaceae) is a tropical tree species native of the Atlantic Forest in the south of Brazil. Previous studies with leaf cell walls of R. jasminoides showed a different proportion of cross-linked glycans compared to what is usually reported for eudicots. However, due to the difficulties of working with whole plant organs, cell suspensions of R. jasminoides, consisting of predominantly undifferentiated cells with mainly primary cell walls, were used to examine cell walls and extracellular soluble polysaccharides (EP) released into the culture medium. Sugar composition and linkage analysis showed homogalacturonans, xylogalacturonans and arabinogalactans to be the predominant EP. In the cell wall, homogalacturonans and arabinogalactans are the major pectins, and xyloglucans and xylans are the major crosslinking glycans. The presence of xylogalacturonans in the R. jasminoides cell cultures seems to be related to the occurrence of a homogeneous cell suspension with loosely attached cells. Although all alkali extractions from the cell walls yielded amounts of xyloglucan that exceed those of the xylans, the latter was found in a proportion that is higher than what has been usually reported for primary cell walls of most eudicots. The xyloglucan from cell walls of cell suspension cultures of R. jasminoides has low fucosylation levels and high proportion of galactosyl residues, a branching pattern commonly found in storage cellwall xyloglucans.

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Section: Resultsmentioning

confidence: 58%

Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

Júnior

Cavalari

Carpita

et al. 2010

Trees

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Thidiazuron-induced somatic embryos, their multiplication, maturation, and conversion in Cinnamomum pauciflorum Nees (Lauraceae)

et al. 2009

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Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

Kretzschmar

Júnior

Braga

2007

In Vitro Cell.Dev.Biol.-Plant

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The primary utilization of carbohydrates by cell suspension cultures of Rudgea jasminoides, a native woody Rubiaceae from tropical forests, was investigated. Sucrose, glucose + fructose, glucose, or fructose were supplied as carbon sources. The growth curves of R. jasminoides cultured in glucose + fructose, glucose, or fructose showed similar patterns to that observed when sucrose was supplied to the cells, except that an increase in dry mass was observed at the beginning of the stationary growth phase in the media containing only one monosaccharide. The increase in hexose levels in the media during the early stages of the cultures indicated extracellular hydrolysis of sucrose, which was further supported by the increase in the activity of acid invertase bound to the cell wall. Glucose was preferentially taken up, whereas uptake of fructose was delayed until glucose was nearly depleted from the medium. Measurements of intracellular sucrose content and cytoplasmatic and vacuolar invertases indicate that the enzymatic activity seems to be correlated with a decrease in the hexose flux into the cells of R. jasminoides. Our results indicate that the behavior of cell suspension cultures of R. jasminoides regarding sugar utilization seems to be similar to other dicotyledonous undifferentiated cell suspension cultures.

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Establishment and Growth of Embryogenic Suspension Cultures of Ocotea catharinensis Mez. (Lauraceae)

Cited by 3 publications

References 11 publications

Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

Thidiazuron-induced somatic embryos, their multiplication, maturation, and conversion in Cinnamomum pauciflorum Nees (Lauraceae)

Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

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