Establishment and neurite outgrowth properties of neonatal and adult rat olfactory bulb glial cell lines

Goodman, M. Nadine; Silver, Jerry; Jacobberger, James W.

doi:10.1016/0006-8993(93)91613-w

Cited by 83 publications

(11 citation statements)

References 63 publications

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“…The basement membrane LEPRE1, a leucine-proline rich chondroitin sulfate proteoglycan, and NID2, an adhesive protein that binds collagen type I, IV, and laminin and is important for the formation of neuromuscular junctions, are both basement membrane-associated proteins (Kohfeldt et al, 1998;Wassenhove-McCarthy and McCarthy, 1999;Fox et al, 2008). OECs were reported to express three other major neurite growth-supporting molecular components of the basement membrane (i.e., collagen type IV, fibronectin, and laminin) (Laurie and Leblond, 1983;Lein et al, 1991;RamonCueto and Nieto-Sampedro, 1992;Doucette, 1996;Kafitz and Greer, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…The bulk of the CTB labeling in the SCARB2 group was observed at 0.5-2 mm distance of the lesion center, approximately at the border of the GFAP-negative area of the scar. Repulsive molecules that are present after a spinal cord lesion, such as the myelin inhibitors, Nogo, MAG, and OMpg, and scar-associated inhibitors, including semaphorins, chondroitin sulfate proteoglycans, and collagens (Niclou et al, 2006;Fawcett et al, 2012;Lee and Zheng, 2012), may counteract the growth permissive environment created by SCARB2 expression. Interestingly, a recent study suggested that scavenger receptors can play a role in myelin clearance (Hendrickx et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In the 1990s, it was shown that OECs stimulate axon regeneration in the injured spinal cord after various types of lesions (Ramon-Cueto and Nieto-Sampedro, 1994;Li et al, 1997;Ramon-Cueto et al, 1998) can remyelinate axons (Franklin et al, 1996;Imaizumi et al, 2000;Radtke et al, 2004) and stimulate neural tissue repair and angiogenesis (Lakatos et al, 2003;Plant et al, 2003;Ramer et al, 2004;.…”

Section: Introductionmentioning

confidence: 99%

“…OECs contribute to regeneration by expressing cell adhesion molecules (NCAM,L1, and by secreting a variety of signaling molecules, including neurotrophic factors (Boruch et al, 2001;Woodhall et al, 2001;Lipson et al, 2003;Santos-Silva et al, 2007), and extracellular matrix molecules (e.g., laminin, clusterin, matrix metalloprotease 2 (MMP2), SPARC, and plasminogen activator inhibitor-1) (Doucette, 1990;Tisay and Key, 1999;Fairless et al, 2005;Au et al, 2007;Liu et al, 2010;Simon et al, 2011). Neurites of cultured primary olfactory neurons are enfolded by OECs (Ramon-Cueto et al, 1993), which promotes their extension (Kafitz and Greer, 1997; Tisay and Key, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Neurites of cultured primary olfactory neurons are enfolded by OECs (Ramon-Cueto et al, 1993), which promotes their extension (Kafitz and Greer, 1997; Tisay and Key, 1999). OECs provide neurotrophic support to adult retinal ganglion cells, embryonic, postnatal, and adult dorsal root ganglion neurons (DRG), postnatal cerebral cortical neurons, and postnatal hippocampal neurons (Goodman et al, 1993;Lipson et al, 2003;Chung et al, 2004;Leaver et al, 2006;Au et al, 2007;Pellitteri et al, 2009;Runyan and Phelps, 2009).…”

Section: Introductionmentioning

confidence: 99%

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A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons

Roet

Franssen

Bree

et al. 2013

Journal of Neuroscience

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Olfactory ensheathing cells (OECs) have neuro-restorative properties in animal models for spinal cord injury, stroke, and amyotrophic lateral sclerosis. Here we used a multistep screening approach to discover genes specifically contributing to the regeneration-promoting properties of OECs. Microarray screening of the injured olfactory pathway and of cultured OECs identified 102 genes that were subsequently functionally characterized in cocultures of OECs and primary dorsal root ganglion (DRG) neurons. Selective siRNA-mediated knockdown of 16 genes in OECs (ADAMTS1, BM385941, FZD1, GFRA1, LEPRE1, NCAM1, NID2, NRP1, MSLN, RND1, S100A9, SCARB2, SERPINI1, SERPINF1, TGFB2, and VAV1) significantly reduced outgrowth of cocultured DRG neurons, indicating that endogenous expression of these genes in OECs supports neurite extension of DRG neurons. In a gain-of-function screen for 18 genes, six (CX3CL1, FZD1, LEPRE1, S100A9, SCARB2, and SERPINI1) enhanced and one (TIMP2) inhibited neurite growth. The most potent hit in both the loss-and gain-of-function screens was SCARB2, a protein that promotes cholesterol secretion. Transplants of fibroblasts that were genetically modified to overexpress SCARB2 significantly increased the number of regenerating DRG axons that grew toward the center of a spinal cord lesion in rats. We conclude that expression of SCARB2 enhances regenerative sprouting and that SCARB2 contributes to OEC-mediated neuronal repair.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons

Roet

Franssen

Bree

et al. 2013

Journal of Neuroscience

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Expression and localization of FGF-1 in the developing rat olfactory system

et al. 1996

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Primary olfactory axons project from the nasal olfactory neuroepithelium to glomeruli in the olfactory bulb where they synapse with mitral cells, the second-order olfactory neurons. We have shown that the heparin-binding growth factor FGF-1 is expressed by olfactory nerve ensheathing cells which surround fascicles of primary olfactory axons en route to the olfactory bulb. These cells are believed to modulate olfactory axon growth between the olfactory neuroepithelium and the olfactory bulb. During late embryogenesis, FGF-1 expression is turned on in the mitral cells, and the FGF-1 peptide becomes confined to layers of synaptic neuropil in the postnatal olfactory bulb. FGF-1 is selectively present in glomeruli and the external plexiform layer. In cultures of olfactory neuroepithelial cells, complexes between FGF-1 and an appropriate activating heparan sulfate proteoglycan stimulated morphological differentiation of both olfactory nerve ensheathing cells and primary sensory olfactory neurons. Thus, the spatiotemporal expression and the functional properties of FGF-1 in this system suggest that this molecule plays an important regulatory role in the formation of the olfactory pathway.

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Do olfactory glia have advantages over Schwann cells for CNS repair?

Franklin

Barnett

1997

J. Neurosci. Res.

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To a large extent the success of axon regeneration and sustained remyelination which distinguishes the PNS from the CNS is attributable to differences in their respective glial environments. For this reason, many have been attracted to the idea that repair of the CNS might be achieved by transplanting Schwann cells into areas of CNS pathology. Schwann cells will not only promote regeneration but will also myelinate axons thereby making them an appropriate cell type to mediate repair of lesions characterised by demyelination as well as axotomy. The recent discovery that olfactory glia are capable of forming myelin sheaths, together with their well-documented ability to support axon regeneration, means that these cells have a range of repair properties similar to that of Schwann cells. It is not clear at present which of these two alternatives, the Schwann cells or the olfactory glial cell, would be of greater benefit for achieving regeneration of axons or remyelination of persistent demyelination following transplantation into the CNS. In this article we review the repair properties of olfactory glia and identify the areas in which their use for repairing the CNS may have advantages over Schwann cells.

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Establishment and neurite outgrowth properties of neonatal and adult rat olfactory bulb glial cell lines

Cited by 83 publications

References 63 publications

A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons

A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons

Expression and localization of FGF-1 in the developing rat olfactory system

Do olfactory glia have advantages over Schwann cells for CNS repair?

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