Establishment, authenticity, and characterization of cervical cancer cell lines

Abstract

Cell lines have been considered excellent research models in many areas of biomedicine and, specifically, in the study of carcinogenesis. However, they cease to be effective models if their behavior changes. Although studies on the cross-contamination of cell lines originating from different tissues have been performed, little is known about cell lines derived from cervical neoplasia. We know that high-risk HPV (HR-HPV) is associated with the development of this type of cancer. This link between HPV infection and cancer was first established over 35 years ago when HPV16 DNA was found to be present in a large proportion of cervical cancer biopsies. The present review paper aims to report the status of the establishment, authenticity, and characterization of cervical cancer (CC) cell lines. This is a systematic review of articles on the establishment, authenticity, and characterization of CC cell lines, published from 1960 to date in the databases and in cell repository databases. 52 cell lines were identified in the literature. Only 25 cell lines were derived from cervical neoplasia, of which only 45.8% have a reported identity test (genomic fingerprint). Despite the increase in the establishment of cell lines of cervical neoplasia and the standards for the regulation of these study models, the criteria for their characterization continue to be diverse.

MUC1 promotes cervical squamous cell carcinoma through ERK phosphorylation-mediated regulation of ITGA2/ITGA3

“…The human cervical squamous cell carcinoma lines SW756, SiHa, CaSki, C33A and ME180 were purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China) [26]. SW756, SiHa and CaSki cells were cultured in RPMI 1640 medium (11875093; Gibco, USA) supplemented with 10% (vol/vol) fetal bovine serum (10099158; Thermo Scientific, USA), penicillin (15140148; 100 U/mL, Gibco) and streptomycin (15140148; 100 mg/mL, Gibco) at 37 °C in a humidified incubator containing 5% CO 2 .…”

Sitotoksisitas Ekstrak Cabe Merah Keriting (Capsicum annum) Terhadap Hela Cell Lines

“…Kanker merupakan salah satu penyakit yang baru banyak diketahui setelah memasuki stadium tiga atau empat, karena deteksi penyakit kanker sejak dini masih jarang dilakukan oleh masyarakat. Kanker jika tidak ditangani dengan segera akan menyebar dan tumbuh secara abnormal di dalam tubuh serta dapat merusak sel normal dan jaringan yang ada disekitarnya [10]. Penelitian ini menggunakan HeLa cell lines sebagai salah satu lini sel kanker serviks.…”

Ligand-Based Drug Design of Genipin Derivatives with Cytotoxic Activity against HeLa Cell Line: A Structural and Theoretical Study

“…CaSki is associated with HPV16 and derived from metastasis, while both CaLo and INBL are associated with HPV18. However, CaLo represents an early stage (stage IIB), while INBL represents a metastatic stage (stage IVB) according to the FIGO classification [28]. This suggests that Genipin could potentially affect cervical cancer cells from both high-risk HPV types (16 and 18) and from different stages of the disease.…”

“…In vitro cytotoxicity activity of Geniposide and Genipin was measured using the sulforhodamine B (SRB) (MP Biomedicals, Irvine, CA, USA) protein staining assay [49][50][51], using three different cervical cancer cell lines CaSki, CaLo, and INBL; and the immortalized keratinocytes HaCaT, as a non-tumorigenic cell line. The CaLo and INBL cell lines [28] were donated by Profesor María de Lourdes Gutiérrez Xicoténcatl from Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública. Briefly, cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, and seeded in a 96-well microtiter plate at a cellular density of 5000 cells/mL, and placed in an incubator (5% CO 2 and 37 • C) for 5 h. Afterward, different concentrations of pure iridoids (0.04128, 0.2064, 1.032, 5.16, 25.8, and 129 µM), of podophyllotoxin positive control (0.00303, 0.00606, 0.01213, 0.02425, 0.04850, and 0.09700 µM), and of cisplatin positive control (0.10400, 0.20781, 0.41563, 0.83125, 1.6625, and 3.325 µM) were added in triplicate and incubated for 72 h. The assays were conducted in three independent experiments.…”