Establishment, Characterization and Expression Pattern of a Spleen Cell Line, SMSP, Derived from Turbot (Scophthalmus maximus)

Xue, Ting; Liu, Yiping; Ge, Xuefeng; Li, Chao

doi:10.1007/s11802-023-5516-6

J. Ocean Univ. China

2023

DOI: 10.1007/s11802-023-5516-6

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Establishment, Characterization and Expression Pattern of a Spleen Cell Line, SMSP, Derived from Turbot (Scophthalmus maximus)

Ting Xue,

Yiping Liu,

Xuefeng Ge

et al.

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2024

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Transcriptome Analysis of Transiently Reversible Cell Vacuolization Caused by Excessive Serum Concentration in Scophthalmus maximus

Song,

Shao,

2024

Biology

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As an important research tool, cell lines play a vital role in life science research, medical research, and drug development. During the culture of the Scophthalmus maximus head kidney (TK) cell line, we found a phenomenon of cell vacuolization caused by excessive serum concentration. Moreover, the vacuolization of the cells gradually disappeared after passage by trypsin digestion. In clarifying the formation mechanism of this reversible cellular vacuolation, transcriptomics was utilized to explore the mechanism of cell vacuolization caused by excessive serum concentration. Transcriptome analysis indicated that excessive serum concentration could cause the up-regulated expression of PORCN and other genes to promote cell proliferation. Compared with cells whose vacuolization disappeared after trypsin digestion and passage, the expression of mitosis-related genes (BUB1, ttk, Mad2, Cdc20, CDK1, CCNB1), nuclear stability-related genes LMNB1 and tissue stress and repair-related genes HMMR in vacuolated cells caused by excessive serum concentration was significantly up-regulated. There is a regulatory system related to adaptation and stress repair in the cells, which can maintain cell stability to a certain extent. This study provides a theoretical basis for the stable culture of fish cell lines and the solution to the problem of cell vacuolation.

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Transcriptome Analysis of Transiently Reversible Cell Vacuolization Caused by Excessive Serum Concentration in Scophthalmus maximus

Song,

Shao,

2024

Biology

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show abstract

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Establishment, Characterization and Expression Pattern of a Spleen Cell Line, SMSP, Derived from Turbot (Scophthalmus maximus)

Cited by 1 publication

References 50 publications

Transcriptome Analysis of Transiently Reversible Cell Vacuolization Caused by Excessive Serum Concentration in Scophthalmus maximus

Transcriptome Analysis of Transiently Reversible Cell Vacuolization Caused by Excessive Serum Concentration in Scophthalmus maximus

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