Establishment, characterization and virus expression of cell lines derived from radiation‐ and virus‐induced lymphomas of C57BL/Ka mice

Lieberman, Miriam; Declève, A.; Ricciardi‐Castagnoli, Paola; Boniver, Jacques; Finn, Olivera J.; Kaplan, Henry S.

doi:10.1002/ijc.2910240208

Cited by 94 publications

(42 citation statements)

References 26 publications

(12 reference statements)

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“…The thymoma cell lines were derived as described previously (9) from tumors induced by intrathymic injection of wild-type A-MuLV, i.e., with Moloney murine leukemia virus as a helper virus (lines named with the prefix M) or with a modified (pseudotype) A-MuLV virus, using radiation leukemia virus (27) as a helper (R prefix). Prefixes also indicate the mouse strain of tumor origin: BL/Ka (K) or BALB/c (B).…”

Section: Resultsmentioning

confidence: 99%

T-cell receptor and immunoglobulin genes are rearranged together in Abelson virus-transformed pre-B and pre-T cells.

Cook¹,

Balaton²

1987

Mol. Cell. Biol.

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We have assessed the state of rearrangement and expression of B-and T-cell antigen receptor genes in cells of Abelson murine leukemia virus-transformed thymomas and other tumors. We found that unrearranged TcR'y genes are expressed, as are unrearranged C,I genes, in pre-T, pre-B, and myeloid cells. We also found TcR-y genes rearranged and expressed in putative pre-T cells and in cells apparently committed to the B-cell lineage. This is in contrast to the data from more mature T-and B-cell tumors. We conclude that in immature lymphoid cells both immunoglobulin and TcR-y genes are accessible for rearrangement. We discuss the implications of these observations for an understanding of the B-T lymphoid differentiation event.B and T lymphoid cells are thought to arise from a common precursor cell, although neither this progenitor nor the developmental point of divergence of the two lineages has been clearly defined (13). Abelson murine leukemia virus (A-MuLV) transforms immature cells of both lineages (8,34). Here we report the use of such transformants to investigate the relationship between developing B and T cells.The major markers of the lymphoid lineages are their antigen receptors. Three gene loci have been described for each antigen receptor complex. For B cells these encode immunoglobulin heavy chain and the K and A light chains, and for T cells they encode a, fi and y polypeptides. Although the role of y has been unclear, it will be referred to here as TcR-y, since the weight of evidence suggests it may be a component of a separate T-cell receptor (TcR) (4, 6). In particular the -y locus shares many featui es with those of the other receptor genes. Their sequences are homologous, and their gene organization is similar, especially in the need for gene rearrangement to juxtapose variable (V) region sequences close to constant regions to allow expression (reviewed (in reference 25a).For B-cell development these rearrangements have been shown to occur as an ordered series of steps. The first event is transcription from the unrearranged C,u gene, which has been taken to indicate an "opening" of this locus, a necessary condition for subsequent rearrangement of the gene. It is noteworthy that this C,u transcription occurs not only in pre-B cells but also in myeloid and T cells (23). The second step, juxtaposition of a diversity (D) region to a joining (J) region of the heavy-chain locus, has been observed in both pre-B and T cells (14,26) While examining receptor gene rearrangement and expression in a series of cloned A-MuLV-induced thymoma cell lines, we observed that TcR-y was often transcribed from unrearranged genes. This was found to occur not only in transformed immature thymocytes but also in myeloid and pre-B cells. This pattern of expression is remarkably like that of unrearranged immunoglobulin p, (Ig,u) genes. Moreover, we observed rearrangement and transcription of apparently legitimate TcR-y genes in cells which also bear immunoglobulin rearrangements thought to be absolutely specific to the B lineage....

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Section: Resultsmentioning

confidence: 99%

T-cell receptor and immunoglobulin genes are rearranged together in Abelson virus-transformed pre-B and pre-T cells.

Cook¹,

Balaton²

1987

Mol. Cell. Biol.

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“…A weakly leukemogenic retrovirus has been isolated from these thymomas (5,15,19), and one such isolate was subsequently rendered highly potent by serial cell-free passages in vivo (13,19). Prevention of thymomas was achieved by active and passive immunization with MuLV antibodies (26), and leukemogenic MuLV has been isolated from several lymphoid cell lines established from these X-ray-induced tumors (18,29). Despite this, infectious MuLV or its footprints have been detected in only a low percentage of primary thymomas, and its presence appears rather elusive (5-7, 10, 11, 17, 19, 26, 31).…”

mentioning

confidence: 99%

Strong selection for cells containing new ecotropic recombinant murine leukemia virus provirus after propagation of C57BL/6 radiation-induced thymoma cells in vitro or in vivo.

Jolicoeur¹,

Rassart²,

Sankar-Mistry³

1983

Mol. Cell. Biol.

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Using the Southern procedure, we have studied the presence of ecotropicspecific murine leukemia viral sequences in genomic DNA isolated from primary X-ray-induced thymomas, from lymphoid cell lines established from them, or from secondary tumors passaged in vivo. We found that primary radiation-induced thymomas and infiltrated spleens do not harbor newly acquired ecotropic provirus. However, additional ecotropic proviruses (which appear recombinant in the gagpol region) could be detected in most of the tumorigenic cell lines established in vitro from them and in tumors arising from subcutaneous transplantation of the primary thymomas. These results suggest that primary radiation-induced thymomas may not be clonal. They also indicate a strong correlation between the presence of ecotropic recombinant proviruses in the genome and the growth ability, both in vitro and in vivo, of specific cells within these thymomas, suggesting a possible mitogenic function for murine leukemia virus.

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“…RadLV was prepared as cell-free extracts of thymomas induced by intrathymic injection of similarly passaged RadLV (14). Preparation of infected thymocytes for RNA preparation and nuclear extraction was as described (10).…”

Section: Methodsmentioning

confidence: 99%

Activation Transcription Factor 1 Involvement in the Regulation of Murine H-2D Expression

Ishiguro

Brown

Meruelo

1997

Journal of Biological Chemistry

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Resistance to radiation leukemia virus-induced leukemia is correlated with an increase in H-2D expression on the thymocyte surface. Recently, it has been shown that elevated H-2D d expression on the infected thymocyte is a result of elevated mRNA transcription and that the transcriptional increase is correlated with elevated levels of a DNA binding activity, H-2 binding factor 1 (H-2 BF1), which recognizes the 5 -flanking sequences (5 -TGACGCG-3 ) of the H-2D d gene. This target for transcription factor binding has been found to be identical in the 5 -regulatory region of 12 rodent class I genes, nine of which have been shown to be functional genes. Furthermore, this cis-element is found 5 of 20 primate class I genes (15 human genes), seven of which are known to be functional. Here, we demonstrate that activation transcription factor 1 (ATF-1) is one component of H-2 BF1. In addition, the levels of ATF-1 mRNA in uninfected and radiation leukemia virus-infected thymocytes parallel those of H-2D d mRNA, and therefore, it is suggested that ATF-1 up-regulates the transcription of the H-2D d gene after radiation leukemia virus infection of thymocytes. Transfection experiments also demonstrate that ATF-1 activates a reporter plasmid that contains the H-2 BF1 motif, but not a reporter lacking this motif. This is the first demonstration of the interaction of ATF-1 with 5 -regulatory sequences of major histocompatibility complex class I genes.

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Establishment, characterization and virus expression of cell lines derived from radiation‐ and virus‐induced lymphomas of C57BL/Ka mice

Cited by 94 publications

References 26 publications

T-cell receptor and immunoglobulin genes are rearranged together in Abelson virus-transformed pre-B and pre-T cells.

T-cell receptor and immunoglobulin genes are rearranged together in Abelson virus-transformed pre-B and pre-T cells.

Strong selection for cells containing new ecotropic recombinant murine leukemia virus provirus after propagation of C57BL/6 radiation-induced thymoma cells in vitro or in vivo.

Activation Transcription Factor 1 Involvement in the Regulation of Murine H-2D Expression

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