Establishment of a ciliated epithelial cell line from human Fallopian tube

Ando, Hironori; Kobayashi, Miya; Toda, Shigeru; Kikkawa, Fumitaka; Masahashi, T; Mizutani, Shigehiko

doi:10.1093/humrep/15.7.1597

Cited by 24 publications

(21 citation statements)

References 22 publications

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“…Previous efforts to recapitulate primary human FTE in culture have produced suboptimal results due to the rapid dedifferentiation and loss of the polarized morphology and the biological behavior of the two sub-populations of epithelium (Ando et al , 2000; Comer et al , 1998; Henriksen et al , 1990; Kervancioglu et al , 1994). …”

Section: Resultsmentioning

confidence: 99%

Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis

et al. 2009

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Recent studies suggest that some serous ovarian carcinomas arise from the fallopian tube epithelium rather than the ovarian surface epithelium. This hypothesis places emphasis on the fallopian tube secretory epithelial cell as a cell-of-origin. Herein we report the development of a novel ‘ex-vivo’ primary human fallopian tube epithelium culture system that faithfully recapitulates the in-vivo epithelium, as demonstrated by morphological, ultrastructural, and immunophenotypic analyses. Mass spectrometry-based proteomics reveals that these cultures secrete proteins previously identified as biomarkers for ovarian cancer. We also utilize this culture system to study the response of the fallopian tube epithelium to genotoxic stress and find that the secretory cells exhibit a distinct response to DNA damage when compared to neighboring ciliated cells. The secretory cells demonstrate a limited ability to resolve the damage over time, potentially leaving them more susceptible accumulation of additional mutagenic injury. This divergent response is confirmed with in-situ studies using tissue samples, further supporting the use of this ex-vivo culture system to investigate fallopian tube epithelial pathobiology. We anticipate that this novel culture system will facilitate the study of serous ovarian carcinoma pathogenesis, and propose that similar culture systems could be developed for other organ-site specific epithelia.

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Section: Resultsmentioning

confidence: 99%

Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis

et al. 2009

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“…Propagation of human immortalized ovarian surface cells (IOSE) 84 and fallopian tube fimbria has been made possible by stable transfection with SV40 T antigen. 85,86 For example, IOSE-29 does not form tumors, while its neoplastic counterpart IOSE29EC forms s.c. and i.p. tumors in SCID mice.…”

Section: Cell Lines and Xenograftsmentioning

confidence: 99%

Epithelial ovarian cancer experimental models

et al. 2013

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Epithelial ovarian cancer (OvCa) is associated with high mortality and, as the majority (>75%) of women with OvCa have metastatic disease at the time of diagnosis, rates of survival have not changed appreciably over 30 years. A mechanistic understanding of OvCa initiation and progression is hindered by the complexity of genetic and/or environmental initiating events and lack of clarity regarding the cell(s) or tissue(s) of origin. Metastasis of OvCa involves direct extension or exfoliation of cells and cellular aggregates into the peritoneal cavity, survival of matrix-detached cells in a complex ascites fluid phase, and subsequent adhesion to the mesothelium lining covering abdominal organs to establish secondary lesions containing host stromal and inflammatory components. Development of experimental models to recapitulate this unique mechanism of metastasis presents a remarkable scientific challenge and many approaches used to study other solid tumors (lung, colon, and breast, for example) are not transferable to OvCa research given the distinct metastasis pattern and unique tumor microenvironment. This review will discuss recent progress in the development and refinement of experimental models to study OvCa. Novel cellular, three-dimensional organotypic, and ex vivo models are considered and the current in vivo models summarized. The review critically evaluates currently available genetic mouse models of OvCa, the emergence of xenopatients, and the utility of the hen model to study OvCa prevention, tumorigenesis, metastasis, and chemoresistance. As these new approaches more accurately recapitulate the complex tumor microenvironment, it is predicted that new opportunities for enhanced understanding of disease progression, metastasis and therapeutic response will emerge.

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“…This becomes critical when small animals like mice are used for experiments. Oviductal epithelial cell lines are established from pathological samples of pig and human oviduct (Boullant et al, 1973;Ando et al, 2000;Lee et al, 2001). To our knowledge, no clonal fibloblastic cell lines are yet established from oviducts, and analysis of epithelial-stromal interaction is very important in epithelial differentiation.…”

Section: Introductionmentioning

confidence: 99%

Characterization of newly established clonal oviductal cell lines and differential hormonal reculation of gene expression

Umezu

Hanazono

Aizawa

et al. 2003

In Vitro Cell.Dev.Biol.-Animal

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Oviductal functions have been studied mainly in primary epithelial cell culture and organ culture. However, secretory cells and ciliated cells coexist in the epithelium, and the small size of the oviduct limits the sources of both epithelial and stromal cells. To circumvent the limits, we attempted to establish clonal cell lines from an oviduct of a p53-deficient mouse. An oviduct was enzymatically digested and cultured in medium containing 10% fetal calf serum supplemented with estradiol-17beta. Morphologically distinct clones (10 epithelial and 4 fibroblastic clones) were established, and all clones expressed estrogen receptor alpha and progesterone receptor. Expression of a mouse oviduct-specific glycoprotein gene as a marker of secretory cells was limited in one clone and was stimulated by estrogens and suppressed by progesterone. Expression of helix factor hepatocyte nuclear factor/forkhead homologue-4 gene as a marker of ciliated cells was limited in two clones and was suppressed by estrogens. The two genes were never coexpressed in any clones. The results strongly suggest that the oviductal epithelium consists of two functionally determined populations. To our knowledge, this is the first establishment of functional clonal cell lines of the oviduct and makes it possible to study independently two oviductal functions, secretion and ciliogenesis.

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Establishment of a ciliated epithelial cell line from human Fallopian tube

Cited by 24 publications

References 22 publications

Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis

Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis

Epithelial ovarian cancer experimental models

Characterization of newly established clonal oviductal cell lines and differential hormonal reculation of gene expression

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