Establishment of a human colony-stimulating-factor-producing cell line from an undifferentiated large cell carcinoma of the lung

Abstract

Two different types of lung cancer, a squamous cell carcinoma designated as OTUK, and an undifferentiated large cell carcinoma named as T3M‐10, were investigated in vitro; both produced a moderate neutrophilia in each patient. In order to analyze this phenomenon, primary cultures were performed. Epithelial cells attached and grew in both of the cultures. The conditioned medium from both tumor cells revealed high colony‐stimulating‐factor (CSF) activities. From T3M‐10 tumor cultures, a new CSF‐producing cell line has been established. T3M‐10 cells have been continuously propagated during the last 28 months and produced CSF. Chromosomal analysis revealed the cell line to be a human aneuploid one with a near‐diploid mode. These results indicate that the two different types of lung cancers produce CSF, which may have stimulated granulopoiesis in vivo in the patients as well.

CDK9 is a prognostic marker and therapeutic target in pancreatic cancer

“…The human pancreatic cancer cell lines BxPC3, 20 Colo 357, 21 and Panc89, 22 kindly provided by Prof. Anna Trauzold (University of Kiel), were cultured in RPMI 1640 Medium (Gibco ® , Thermo Fisher Scientific, Schwerte, Germany) containing 10% fetal calf serum (Biochrom AG, Merck Millipore, Darmstadt, Germany), 1% sodium pyruvate, 1% L-glutamine, and 1% penicillin/ streptomycin (Thermo Fisher Scientific). The human pancreatic duct epithelium (HPDE) cell line HPDE6-E6E7-c7, 23 kindly provided by Ming-Sound Tsao (Toronto), was cultured in a 1:1 mixture of Keratinocyte SFM medium and RPMI 1640 Medium (GlutaMAX™; Gibco, Thermo Fisher Scientific) containing 0.025% bovine pituitary extract, 2.5 µg/L epidermal growth factor (Gibco, Thermo Fisher Scientific), 10% fetal calf serum (Biochrom AG, Merck Millipore), and 1% penicillin/streptomycin (Thermo Fisher Scientific).…”

Tumor-related leucocytosis and chemotherapy-induced neutropenia: Linked or independent prognostic factors for advanced non-small cell lung cancer?

“…After Asano et al [14] published their report on granulocyte-colony stimulating factor (G-CSF)-producing lung carcinoma in 1977, more than 40 hematopoietic cytokines producing lung carcinoma have been identified to date. Several hematopoietic cytokines have been identified from tumor cells or established tumor cell lines [15][16][17][18][19]. The majority of lung carcinomas have been associated with G-CSF production [20,21], and a few cases have been associated with granulocyte-macrophage-colony stimulating factor (GM-CSF) [22] and interleukin-6 (IL-6) production [23].…”

Neutrophilia Associated with Anaplastic Carcinoma of the Thyroid: Production of Macrophage Colony-Stimulating Factor (M-CSF) and lnterleukin-6

“…After the first report showing that soluble factors produced by carcinoma cells caused neutrophilia in tumor-transplanted mice (1), the contribution of colony-stimulating factors (CSFs) to neutrophilia associated with cancers has been extensively investigated and reported on a case report basis. In neutrophilia in patients with thyroid carcinoma, about 12 cases reports, including 3 proceedings, and 8 reports studying the cell lines established from the tumor cells of patients have been recorded (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13).…”