Establishment of a human preadipose cell line, HPB‐AML‐I: Refractory to PPARγ‐mediated adipogenic stimulation

Abstract

In this study, we established a unique cell line, HPB-AML-I (AML-I), from peripheral blood mononuclear cells collected from a patient with acute myeloid leukemia (AML: M1). Morphological and phenotypical analyses of the established AML-I cells demonstrated that they belong to a preadipocyte cell line as indicated by their storage of lipid droplets and expression of surface antigens similar to those found on bone marrow stromal cells (MSC). Through cell culture under adipogenic conditions, effective differentiation of AML-I cells into adipocytes was induced by an adipogenesis inducing cocktail (INC) made up of a mixture of methylisobutylxanthine, hydrocortisone, and indomethacin. By contrast, activation of peroxisome proliferator-activated receptor (PPARgamma), which plays a key role in lipids metabolism and is highly expressed in AML-I cells, decreased the number of lipid droplets in AML-I cells. Here we report the establishment of a unique human derived-preadipocyte cell line, AML-I, and its bi-directional adipogenic response to different type stimulation, i.e., one is a refractory response to troglitazone, a well-known adipogenic stimulator, and a positive response to INC, an adipogenesis induction cocktail. These results suggest that, based on the adipogenic response, there might be some distinct lineages in human adipocytes and that the unique differentiation of AML-I cells should be useful for analyzing both the differentiation and regulation of human preadipocytes.

Oleate promotes differentiation of chicken primary preadipocytes in vitro

“…Adipocyte differentiation has been extensively studied in mammalian preadipocyte cell lines, such as 3T3-L1 and 3T3-F442A cells [1,2]. These studies have revealed a complicated regulatory network of transcription factors underlies adipocyte differentiation.…”

Growth arrest and apoptosis induced by quercetin is not linked to adipogenic conversion of human preadipocytes

“…7. As we have previously shown that AML-I cells can differentiate into adipocytes with an increase in cytoplasmic lipid droplets after adipogenic stimulation [26], these results suggested that quercetin might promote adipogenic conversion of preadipocytes.…”

“…The human preadipocyte cell line AML-I is possibly derived from a lineage of human bone marrow stromal cells as described previously [26]. Using the AML-I cell model, we investigated the effect of quercetin on the proliferation and differentiation of preadipocytes in vitro.…”

The HPB-AML-I cell line possesses the properties of mesenchymal stem cells

“…In spite of its establishment from the peripheral blood mononuclear cells (PBMCs) of a case with acute myeloid leukemia (AML)-M1, this cell line reportedly has the features of spindle-like morphology and plastic adherence [12]. The detached HPB-AML-I cells were surprisingly capable of proliferating and adhering to plastic surfaces after passage.…”

“…The detached HPB-AML-I cells were surprisingly capable of proliferating and adhering to plastic surfaces after passage. Immunophenotypic analysis of HPB-AML-I demonstrated the absence of hematopoietic cell-surface antigens and showed that this cell line resembles marrow stromal cells [12]. Moreover, in the presence of methylisobutylxanthine, hydrocortisone, and indomethacin, but not troglitazone, an increase in the number of lipid droplets was observed in these cells [12].…”