Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation

Ben‐Bassat, Hannah; Shlomai, Zipora; Kohn, Gertrude; Prokocimer, Miron

doi:10.1016/0165-4608(90)90148-4

Cited by 24 publications

(15 citation statements)

References 23 publications

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“…Experiments were performed on cells from the lymphoblastoid Loucy cell line (DSMZ, ACC394) 17 . A CNV analysis has been performed on this cell line as part of the COSMIC project 18, 19 .…”

Section: Methodsmentioning

confidence: 99%

Performance of four modern whole genome amplification methods for copy number variant detection in single cells

Deleye

Tilleman

Plaetsen

et al. 2017

Sci Rep

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Whole genome amplification (WGA) has become an invaluable tool to perform copy number variation (CNV) detection in single, or a limited number of cells. Unfortunately, current WGA methods introduce representation bias that limits the detection of small CNVs. New WGA methods have been introduced that might have the potential to reduce this bias. We compared the performance of PicoPLEX DNA-Seq (Picoseq), DOPlify, REPLI-g and Ampli-1 WGA for aneuploidy screening and copy number analysis using shallow whole genome massively parallel sequencing (MPS), starting from single or a limited number of cells. Although the four WGA methods perform differently, they are all suited for this application.

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“…Experiments were performed on cells from the lymphoblastoid Loucy cell line (DSMZ, ACC394) 17 . A CNV analysis has been performed on this cell line as part of the COSMIC project 18, 19 .…”

Section: Methodsmentioning

confidence: 99%

Performance of four modern whole genome amplification methods for copy number variant detection in single cells

Deleye

Tilleman

Plaetsen

et al. 2017

Sci Rep

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“…This study was performed on cells derived from the female LOUCY cell line (DSMZ, ACC394)6. A reference 180 K arrayCGH profile (Agilent Technologies) was obtained from unamplified genomic DNA from this cell line (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Fastq files of the samples were automatically analyzed using the Vivar software67. The Vivar software performs CNA detection using the QDNAseq algorithm8.…”

Section: Methodsmentioning

confidence: 99%

Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing

Deleye

Coninck

Dheedene

et al. 2016

Sci Rep

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Starting from only a few cells, current whole genome amplification (WGA) methods provide enough DNA to perform massively parallel sequencing (MPS). Unfortunately, all current WGA methods introduce representation bias which limits detection of copy number aberrations (CNAs) smaller than 3 Mb. A recent WGA method, called TruePrime single cell WGA, uses a recently discovered DNA primase, TthPrimPol, instead of artificial primers to initiate DNA amplification. This method could lead to a lower representation bias, and consequently to a better detection of CNAs. The enzyme requires no complementarity and thus should generate random primers, equally distributed across the genome. The performance of TruePrime WGA was assessed for aneuploidy screening and CNA analysis after MPS, starting from 1, 3 or 5 cells. Although the method looks promising, the single cell TruePrime WGA kit v1 is not suited for high resolution CNA detection after MPS because too much representation bias is introduced.

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“…The cells were cultured for 48 h in the absence or presence of TI, and the expression of BAFF was examined by RT-PCR. Among T cell lines thus far examined, a T cell line named Loucy, which was previously established from T cell acute lymphoblastic leukemia (30), showed robust induction of BAFF in response to the stimulation. On the other hand, other T cell lines as well as a human mononuclear cell line, THP-1, expressed BAFF almost constitutively (Fig.…”

Section: Inducible Expression Of Baff In Loucymentioning

confidence: 99%

Aberrant expression of BAFF in T cells of systemic lupus erythematosus, which is recapitulated by a human T cell line, Loucy

Yoshimoto

Takahashi

Ogasawara

et al. 2006

International Immunology

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B cell-activating factor of the tumor necrosis factor (TNF) family, or BAFF, is mainly produced in monocytes and dendritic cells, and indispensable for proliferation, differentiation and survival of B cells. BAFF is a type II membrane-bound protein and the extracellular C-terminal fragment is released from the cells as soluble BAFF (sBAFF), which binds to specific receptors on B cells. Accumulating evidence suggests that BAFF plays an important role in the pathogenesis of autoimmune diseases, such as systemic lupus erythematosus (SLE). In this study, we developed a sensitive sandwich ELISA system to quantify the amount of sBAFF using our own mAb. Treatment of peripheral T cells of SLE patients with an anti-CD3 antibody triggered robust expression of BAFF and subsequent release of sBAFF from the cells. On the other hand, the stimulus induced only marginal elevation of sBAFF from normal T cells.

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Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation

Cited by 24 publications

References 23 publications

Performance of four modern whole genome amplification methods for copy number variant detection in single cells

Performance of four modern whole genome amplification methods for copy number variant detection in single cells

Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing

Aberrant expression of BAFF in T cells of systemic lupus erythematosus, which is recapitulated by a human T cell line, Loucy

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