Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1.

Abstract

A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologically diagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 colon of K-ras was detected, expression of c-erbB-2 product and MUC 1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.extrahepatic bile duct carcinoma; cell line; TFK-1; c-erbB-2; MUC1In cases of bile duct carcinoma (BDC), massive infiltration of tumor cells along the bile duct tree and a multicentric origin of tumors are very frequently observed (Suzuki et al. 1989). Therefore curative resection of BDC is usually difficult, resulting in a poor prognosis of BDC patients. The limits of surgery for BDC indicate the need for adjuvant therapy to control residual BDC tissues. To develop an effective therapy for BDC, it is necessary to establish cell lines for

Establishment and characterisation of six human biliary tract cancer cell lines

“…However, cell lines originated from biliary tract cancers have rarely been reported. Ten human extrahepatic bile duct carcinoma cell lines (SK-ChA-1 (Knuth et al, 1985), KMBC (Yano et al, 1992), and OCUCh-LM1 (Yamada et al, 1995), TFK-1 (Saijyo et al, 1995), ICBD-1 (Takiyama et al, 1998), HBDC (Jiao et al, 2000), SCK, JCK, Cho-CK, Choi-CK (Kim et al, 2001a)) and three gall bladder carcinoma cell lines (MZ-ChA-1 and MzChA-2 (Knuth et al, 1985), OCUG-1 (Yamada et al, 1997) have been reported in the world literature.…”

Interleukin 6 upregulates myeloid cell leukemia‐1 expression through a STAT3 pathway in cholangiocarcinoma cells†

“…The human cholangiocarcinoma cell lines KMCH-1, 19 TFK-1, 20 Mz-ChA-1, 21 and KMBC 22 were cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, penicillin G (100,000 U/L), streptomycin (100 mg/L), and gentamycin (100 mg/L). 11,23 H69 cells, immortalized human cholangiocytes, were kindly provided by Dr. D. Jefferson (Tufts University, Boston, MA) and cultured as previously described.…”

Differential expression of cyclooxygenase-2 (COX-2) in human bile duct epithelial cells and bile duct neoplasm

“…Human hepatobiliary carcinoma cell lines used in this study were HuCC-T1, 49 derived from moderately differentiated adenocarcinoma from intrahepatic bile duct carcinoma (IHBC); HuH-28, 50 undifferentiated adenocarcinoma of IHBC; TFK-1, 51 partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma of extrahepatic bile duct carcinoma (EHBC); NCCC-1, well-differentiated adenocarcinoma of EHBC; and MzChA-1, 52 a highly differentiated papillary adenocarcinoma of GBC.…”