Establishment of a peritoneal mesothelial cell line from a transgenic rat harbouring the temperature-sensitive simian virus 40 large T-antigen gene

Hotta, Yoko; Kaneko, Kayo; Inuma, Jiro; Inami, Yuko; Aruga, Seiki; Shimaoka, Tetsutaro; Sekiguchi, Y.; Io, Hiroaki; Hamada, Chikuma; Obinata, Masuo; Ueda, Masatsugu; Tomino, Yasuhiko

doi:10.1093/ndt/gfp742

Cited by 8 publications

(7 citation statements)

References 24 publications

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“…Recently, we established transgenic rats that express the temperature-sensitive tsA58 mutant of the simian virus 40 large T-antigen, which serves as a source of immortalized rat cell lines [44]. This cell line might be a useful tool for various types of in vitro biological research on peritoneal dialysis.…”

Section: Mesothelial Transplantationmentioning

confidence: 99%

Mechanisms and interventions in peritoneal fibrosis

Tomino

2011

Clin Exp Nephrol

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Peritoneal dialysis (PD) is an attractive treatment for patients with end-stage kidney disease (ESKD). However, long-term peritoneal dialysis is associated with development of functional and structural alterations of the peritoneal membrane. Several factors are implicated in the development of peritoneal fibrosis in PD patients. Inflammatory cytokines, which are induced in the peritoneal cavity during peritonitis, may also induce chronic inflammation and fibrosis. Transforming growth factor β1 (TGF-β1) is generally considered to play an important role in peritoneal fibrosis. The objective of this review is to summarize the mechanisms of peritoneal fibrosis using in vitro and in vivo studies, and the current status and future prospects of interventions in the peritoneal fibrosis.

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Section: Mesothelial Transplantationmentioning

confidence: 99%

Mechanisms and interventions in peritoneal fibrosis

Tomino

2011

Clin Exp Nephrol

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“…To determine the paracrine effect of transplanted mesothelial cells, we injected temperature-sensitive mesothelial cells, of which proliferation is controlled by temperature. The TSMCs were not proliferated but only differentiated at what might be an interperitoneal temperature of 38°C in the rats [15]. The characteristics of TSMCs might be one of the reasons for results to be different from previous reports [20–22].…”

Section: Discussionmentioning

confidence: 99%

“…We established TSMC lines from temperature-sensitive SV40 large T antigen gene transgenic rats [15]. TSMCs progressively grow at 33°C, and are suspended and differentiated at 38°C, whereas the cells are continuously sustaining the native morphological and functional characteristics of primary mesothelial cells.…”

Section: Methodsmentioning

confidence: 99%

“…The relevance of the pro-fibrotic growth factor TGF-β in the failure of ultrafiltration induced by PD was underscored in a rat model in which the TGF-β gene was transduced to the peritoneum, where it was associated with a decrease in peritoneal function [14]. We established temperature-sensitive immortalized mesothelial cell (TSMC) lines, which are not proliferated but only differentiated at 38°C or more such as peritoneal cavity, from temperature-sensitive SV40 large T antigen gene transgenic rats [15]. The repeatedly subcultured mesothelial cells are retaining the characteristics of primary mesothelial cell [15].…”

Section: Introductionmentioning

confidence: 99%

“…We established temperature-sensitive immortalized mesothelial cell (TSMC) lines, which are not proliferated but only differentiated at 38°C or more such as peritoneal cavity, from temperature-sensitive SV40 large T antigen gene transgenic rats [15]. The repeatedly subcultured mesothelial cells are retaining the characteristics of primary mesothelial cell [15]. The mesothelial cells transplanted into the peritoneal cavity play a role in peritoneal homeostasis and immunoregulation without their proliferation.…”

Section: Introductionmentioning

confidence: 99%

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Paracrine effects of transplanted mesothelial cells isolated from temperature-sensitive SV40 large T-antigen gene transgenic rats during peritoneal repair

Kanda

Hamada

Kaneko

et al. 2013

Nephrology Dialysis Transplantation

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BackgroundThe prevention and restoration of peritoneal damage is a critical mission in peritoneal dialysis (PD). Transplantation of mesothelial cells has been suggested to suppress peritoneal injury during PD. Few studies have examined the efficacy and safety of cell transplantation. We evaluated the paracrine effects of mesothelial transplantation during peritoneal repair using immortalized temperature-sensitive mesothelial cells (TSMCs) in chlorhexidine gluconate (CG)-induced peritoneal fibrosis rats.MethodsContinuous-infusion pumps containing 8% CG were placed into the abdominal cavity for 21 days. After the removal of the pumps, the TSMCs were injected into the peritoneal cavity at Day 22 (Tx-1 group) or 29 (Tx-2 group). Morphological findings and mRNA expressions of regeneration-related factors were examined at Days 22, 29 and 35.ResultsPeritoneal thickness was aggravated in the Tx-1 group. Levels of transforming growth factor (TGF)-β, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 mRNA in the Tx-1 group at Day 35 were comparable with those at Day 22. The levels of Snail, B-Raf and ERK-1, markers of epithelial to mesenchymal transition and of the RAS/MAPK pathway in the Tx-1 group, were significantly higher than those in the Tx-2 group. TGF-β and VEGF were produced from the transplanted mesothelial cells and the surrounding cells in the Tx-1 group.ConclusionIt appears that the paracrine effect of transplanted mesothelial cells during peritoneal repair is associated with its surrounding condition. It is important to determine the most appropriate time for developing peritoneal repair through mesothelial transplantation.

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Peritoneal Structure and Changes as a Dialysis Membrane After Peritoneal Dialysis

Selgas

Honda

López–Cabrera

et al. 2023

Nolph and Gokal's Textbook of Peritoneal Dialysis

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Establishment of a peritoneal mesothelial cell line from a transgenic rat harbouring the temperature-sensitive simian virus 40 large T-antigen gene

Abstract: We established a novel, conditionally immortalized MC line that continuously retained the characteristics of primary cultured peritoneal MCs. This cell line might be a useful tool for various types of in vitro biological research on peritoneal dialysis.

Cited by 8 publications

References 24 publications

Mechanisms and interventions in peritoneal fibrosis

Mechanisms and interventions in peritoneal fibrosis

Paracrine effects of transplanted mesothelial cells isolated from temperature-sensitive SV40 large T-antigen gene transgenic rats during peritoneal repair

Peritoneal Structure and Changes as a Dialysis Membrane After Peritoneal Dialysis

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