Establishment of a porcine parvovirus (PPV) DNA standard and evaluation of a new LightCycler nested-PCR assay for detection of PPV

Prikhod'ko, Grigori G.; Reyes, Herbert; Vasilyeva, Irina; Busby, Thomas F.

doi:10.1016/s0166-0934(03)00130-7

Cited by 9 publications

(9 citation statements)

References 9 publications

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“…B19 DNA was purified with a filtration method as described previously 24 or with QIAamp, DNA mini kit (Qiagen, Valencia, CA). PCR primers were designed for the conservative regions located by aligning all available B19 sequences.…”

Section: Methodsmentioning

confidence: 99%

“…Amplification of cDNA was carried out in a 50‐µL PCR mixture containing 1 µL of eLONGase (Invitrogen); 400 nmol per L forward and reverse primers; ‐9 (5′‐GTTTTTTGTGAGCTAACTA) and (5′‐GTCCCA CACATAATCAACCCCAAC), encoded at nucleotides 563 to 581 and 2390 to 2367, respectively; 0.2 mmol per L of each dNTP; 60 mmol per L Tris‐SO 4 , pH 9.1; and 18 mmol per L (NH 4 ) 2 SO 4 . The cycling conditions were set as described earlier: 24 35 cycles at 94°C for 5 seconds, 52°C for 5 seconds, and 72°C for 25 seconds. RT‐PCR products were examined by 1.2 percent agarose gel electrophoresis analysis with ethidium bromide staining.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of a new LightCycler reverse transcription–polymerase chain reaction infectivity assay for detection of human parvovirus B19 in dry‐heat inactivation studies

et al. 2005

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Evaluation of a new LightCycler reverse transcription–polymerase chain reaction infectivity assay for detection of human parvovirus B19 in dry‐heat inactivation studies

et al. 2005

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“…PEDV, TGEV, AIV, and PRV are enveloped viruses, while PPV and PoRV are non-enveloped ones. 49 − 54 Thus, the high recoveries also showed that the method could be used for the detection of different types of viruses. It should be noted that the recovery rates of the method varied for different viruses.…”

Section: Resultsmentioning

confidence: 95%

Detection of SARS-CoV-2 and Other Viruses in Wastewater: Optimization and Automation of an Aluminum Hydroxide Adsorption–Precipitation Method for Virus Concentration

Zhang

Yang

2022

ACS EST Water

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This study aimed to provide a low-cost technique for virus detection in wastewater by improving an aluminum hydroxide adsorption–precipitation method. The releasing efficiency of viruses trapped by the aluminum hydroxide precipitates was improved by adding ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) to dissolve the precipitates at a Na 2 EDTA·2H 2 O:AlCl 3 molar ratio of 1.8–3.6. The recovery rates of the improved method for seven viruses, including SARS-CoV-2-abEN pseudovirus and six animal viruses, were 5.9–22.3% in tap water and 4.9–35.1% in wastewater. Rotavirus A (9.0–4.5 × 10 3 copies/mL), porcine circovirus type 2 (5.8–6.4 × 10 5 copies/mL), and porcine parvovirus (5.6–2.7 × 10 4 copies/mL) were detected in China’s pig farm wastewater, while rotavirus A (2.0 × 10 3 copies/mL) was detected in hospital wastewater. SARS-CoV-2 was detected in hospital wastewater (8.4 × 10 2 to 1.4 × 10 4 copies/mL), sewage (6.4 × 10 to 2.3 × 10 3 copies/mL), and river water (6.6 × 10 to 9.3 × 10 copies/mL) in Nepal. The method was automized, with a rate of recovery of 4.8 ± 1.4% at a virus concentration of 10 2 copies/mL. Thus, the established method could be used for wastewater-based epidemiology with sufficient sensitivity in coping with the COVID-19 epidemic and other virus epidemics.

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“…PPV is a nonenveloped ssDNA virus that is responsible for pig intestine infections, which is the most frequent cause of swine reproductive failure . This virus is also often used as a model for the human B19 parvovirus, which is associated with a number of conditions, such as fifth disease in children and arthritis and arthralgias in adults . The PPV infection cycle has been pieced together from traditional pathological observation techniques. − Briefly, upon infection, viruses bind to specific target receptors on the cell membrane surface where the parvovirus capsid is internalized through clathrin-coated pits and then transferred through the endocytic pathway.…”

Section: Introductionmentioning

confidence: 99%

Changes in Membrane Dielectric Properties of Porcine Kidney Cells Provide Insight into the Antiviral Activity of Glycine

Habibi

Joshi

et al. 2020

Langmuir

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The ability to monitor the status and progression of viral infections is important for development and screening of new antiviral drugs. Previous research illustrated that the osmolyte glycine (Gly) reduced porcine parvovirus (PPV) infection in porcine kidney (PK-13) cells by stabilizing the capsid protein and preventing virus capsid assembly into viable virus particles. Dielectrophoresis (DEP) was examined herein as a noninvasive, electric field- and frequency-dependent tool for real-time monitoring of PK-13 cell responses to obtain information about membrane barrier functionality and polarization. DEP responses of PK-13 cells were compared to those of PPV-infected cells in the absence and presence of the osmolyte glycine. With infection progression, PK-13 DEP spectra shifted toward lower frequencies, reducing crossover frequencies (f CO). The spherical single-shell model was used to extract PK-13 cell dielectric properties. Upon PPV infection, specific membrane capacitance increased over the time progression of virus attachment, penetration, and capsid protein production and assembly. Following glycine treatment, the DEP spectra displayed attenuated f CO and specific membrane capacitance values shifted back toward uninfected PK-13 cell values. These results suggest that DEP can be used to noninvasively monitor the viral infection cycle and screen antiviral compounds. DEP can augment traditional tools by elucidating membrane polarization changes related to drug mechanisms that interrupt the virus infection cycle.

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Establishment of a porcine parvovirus (PPV) DNA standard and evaluation of a new LightCycler nested-PCR assay for detection of PPV

Cited by 9 publications

References 9 publications

Evaluation of a new LightCycler reverse transcription–polymerase chain reaction infectivity assay for detection of human parvovirus B19 in dry‐heat inactivation studies

Evaluation of a new LightCycler reverse transcription–polymerase chain reaction infectivity assay for detection of human parvovirus B19 in dry‐heat inactivation studies

Detection of SARS-CoV-2 and Other Viruses in Wastewater: Optimization and Automation of an Aluminum Hydroxide Adsorption–Precipitation Method for Virus Concentration

Changes in Membrane Dielectric Properties of Porcine Kidney Cells Provide Insight into the Antiviral Activity of Glycine

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