Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures

Fietz, Agnes; Hurst, José; Joachim, Stephanie C.; Schnichels, Sven

doi:10.1016/j.xpro.2023.102443

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Retinal explants were isolated and kept under culture conditions as described before [39,40]. Briefly, after removing the cornea, lens, and vitreous, a clover-leaf-like structure was generated.…”

Section: Isolation and Cultivation Of Retinal Explantsmentioning

confidence: 99%

Blue Light Damage and p53: Unravelling the Role of p53 in Oxidative-Stress-Induced Retinal Apoptosis

Fietz,

Corsi,

Hurst

et al. 2023

Antioxidants

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In the digital age, the widespread presence of electronic devices has exposed humans to an exceptional amount of blue light (BL) emitted from screens, LEDs, and other sources. Studies have shown that prolonged exposure to BL could have harmful effects on the visual system and circadian rhythm regulation. BL is known to induce oxidative stress, leading to DNA damage. Emerging research indicates that BL may also induce cell death pathways that involve the tumor-suppressor protein p53. Activated p53 acts as a transcription factor to regulate the expression of genes involved in cell cycle arrest, DNA repair, and apoptosis. This study aimed to explore the implication of p53 in BL-caused retinal damage, shedding light on the potential mechanisms of oxidative-stress-induced retinal diseases. BL-exposed porcine retinal cultures demonstrated increased p53- and caspase-mediated apoptosis, depending on exposure duration. Direct inhibition of p53 via pifithrin α resulted in the prevention of retinal cell death. These findings raise concerns about the long-term consequences of the current daily BL exposure and its potential involvement in various pathological conditions, including oxidative-stress-based retinal diseases like age-related macular degeneration. In addition, this study paves the way for the development of novel therapeutic approaches for oxidative-stress-based retinal diseases.

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Section: Isolation and Cultivation Of Retinal Explantsmentioning

confidence: 99%

Blue Light Damage and p53: Unravelling the Role of p53 in Oxidative-Stress-Induced Retinal Apoptosis

Fietz,

Corsi,

Hurst

et al. 2023

Antioxidants

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Degradable and Biocompatible Magnesium Zinc Structures for Nanomedicine: Magnetically Actuated Liposome Microcarriers with Tunable Release

Peter,

Kadiri,

Goyal

et al. 2024

Adv Funct Materials

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Inorganic therapeutic carriers and implants should not only be biocompatible, but should also degrade under physiological conditions. Ideally, the time of the degradation can be controlled, and ideally the degradation products are fully biocompatible and metabolized by the body. This proves a challenge for carriers used in nanomedicine, including microswimmers and nanorobotic systems destined for targeted delivery, as these generally require inorganic materials to enable coupling to external fields. Taking inspiration from macroscopic orthopedic implants that are made from magnesium (Mg) and zinc (Zn) and that are fully biocompatible and degradable, it is showed the growth of complex microstructures, including micropropellers, containing Mg and Zn. By varying the content of Mg, the corrosion time of the microstructures can be tuned from hours to over a month. Incorporation of biocompatible hard‐magnetic iron (Fe)‐platinum (Pt) permits the controlled motion of the micropropellers. The surface of the MgZn structures can be functionalized with liposomes, rendering the structures microcarriers that allow for a time‐dependent release of their cargo as a results of their degradation in aqueous environments. This suggests a powerful platform for targeted drug or gene delivery, that can be integrated with established systems for magnetic actuation and transfection.

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Co-cultivation of primary porcine RPE cells and neuroretina induces inflammation: a potential inflammatory AMD-model

Fietz,

Schnichels,

Hurst

2023

Sci Rep

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One common aspect in the pathology of many retinal diseases like age-related macular degeneration (AMD) is the death of retinal pigment epithelium (RPE) cells. RPE cells are essential for photoreceptor survival as they recycle and remove compounds of the visual cycle and secrete protective cytokines. Studying RPE cells is crucial to improve our understanding of retinal pathologies, yet only a few retinal ex vivo models include them or do so only indirectly. Besides the positive effects in indirect co-cultivation models, also a slight inflammation was observed. In this study we developed an ex vivo model consisting of a primary porcine RPE monolayer directly co-cultured with porcine retinal organ cultures, to investigate and simulate inflammatory retinal diseases, such as (dry) AMD. The direct co-cultivation resulted in immune reactivity (enhanced expression of pro-inflammatory cytokines e.g., IL-1β, IL-6,IL-8) and cell death. These effects were evaluated for the retinal explant as well as for the RPE-monolayer to further understand the complex interactions between these two compartments. Taken together, this ex vivo model can be used to study inflammatory retinal diseases like AMD as well as the rejection observed after RPE-transplantation.

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Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures

Cited by 3 publications

References 6 publications

Blue Light Damage and p53: Unravelling the Role of p53 in Oxidative-Stress-Induced Retinal Apoptosis

Blue Light Damage and p53: Unravelling the Role of p53 in Oxidative-Stress-Induced Retinal Apoptosis

Degradable and Biocompatible Magnesium Zinc Structures for Nanomedicine: Magnetically Actuated Liposome Microcarriers with Tunable Release

Co-cultivation of primary porcine RPE cells and neuroretina induces inflammation: a potential inflammatory AMD-model

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