Establishment of a procedure for bacterial spot inoculation and assessment in processing tomato field trials

Pontes, Nadson de Carvalho; Nascimento, A. R.; Moita, A. W.; Maffia, Luiz A.; Oliveira, José Rogério de; Quezado-Duval, A. M.

doi:10.1007/s40858-015-0039-3

Cited by 4 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Implications of this widespread use of Cu based agrochemicals could mean increased evolutionary pressure on the development of Cu-tolerant Xanthomonas strains in tomato growing regions. This is well-documented and is a growing concern for tomato producers and scientists working in this area. ,,− Thus, identifying alternatives to Cu compounds that can manage Cu tolerant strains of Xanthomonas spp. and would also have minimal impact in soil accumulation is critical.…”

Section: Discussionmentioning

confidence: 99%

Magnesium Oxide Nanomaterial, an Alternative for Commercial Copper Bactericides: Field-Scale Tomato Bacterial Spot Disease Management and Total and Bioavailable Metal Accumulation in Soil

Liao

Huang

Carvalho

et al. 2021

Environ. Sci. Technol.

View full text Add to dashboard Cite

Copper (Cu) is the most extensively used bactericide worldwide in many agricultural production systems. However, intensive application of Cu bactericide have increased the selection pressure toward Cu-tolerant pathogens, including Xanthomonas perforans, the causal agent of tomato bacterial spot. However, alternatives for Cu bactericides are limited and have many drawbacks including plant damage and inconsistent effectiveness under field conditions. Also, potential ecological risk on nontarget organisms exposed to field runoff containing Cu is high. However, due to lack of alternatives for Cu, it is still widely used in tomato and other crops around the world in both conventional and organic production systems. In this study, a Cu-tolerant X. perforans strain GEV485, which can tolerate eight tested commercial Cu bactericides, was used in all the field trials to evaluate the efficacy of MgO nanomaterial. Four field experiments were conducted to evaluate the impact of intensive application of MgO nanomaterial on tomato bacterial spot disease severity, and one field experiment was conducted to study the impact of soil accumulation of total and bioavailable Cu, Mg, Mn, and Zn. In the first two field experiments, twice-weekly applications of 200 μg/mL MgO significantly reduced disease severity by 29–38% less in comparison to a conventional Cu bactericide Kocide 3000 and 19–30% less in comparison to the water control applied at the same frequency (p = 0.05). The disease severity on MgO twice-weekly was 12–32% less than Kocide 3000 + Mancozeb treatment. Single weekly applications of MgO had 13–19% higher disease severity than twice weekly application of MgO. In the second set of two field trials, twice-weekly applications of MgO at 1000 μg/mL significantly reduced disease severity by 32–40% in comparison to water control applied at the same frequency (p = 0.05). There was no negative yield impact in any of the trials. The third field experiment demonstrated that application of MgO did not result in significant accumulation of total and bioavailable Mg, Mn, Cu, or Zn in the root-associated soil and in soil farther away from the production bed compared to the water control. However, Cu bactericide contributed to significantly higher Mn, Cu, and Zn accumulation in the soil compared to water control (p = 0.05). This study demonstrates that MgO nanomaterial could be an alternative for Cu bactericide and have potential in reducing risks associated with development of tolerant strains and for reducing Cu load in the environment.

show abstract

Section: Discussionmentioning

confidence: 99%

Magnesium Oxide Nanomaterial, an Alternative for Commercial Copper Bactericides: Field-Scale Tomato Bacterial Spot Disease Management and Total and Bioavailable Metal Accumulation in Soil

Liao

Huang

Carvalho

et al. 2021

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…; Pontes et al . ). Inoculum was applied to plants to the point of run‐off 20 days after transplanting, ensuring thorough coverage of both the upper and lower leaf surfaces.…”

Section: Methodsmentioning

confidence: 97%

“…Bacterial inoculum was prepared to approximately 1 9 10 8 CFU per ml in 10 mmol l À1 MgSO 4 Á7H 2 O, from 48-h GNA cultures (Pernezny et al 2008;Pontes et al 2015). Inoculum was applied to plants to the point of run-off 20 days after transplanting, ensuring thorough coverage of both the upper and lower leaf surfaces.…”

Section: Confirmation Of Copper Tolerance In Vivomentioning

confidence: 99%

Media pH and media type can significantly affect the reliability of in vitro copper tolerance assessments of Pseudomonas syringae pv. tomato

Griffin

Brown

Gambley³

2018

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

Intervals and Number of Applications of Acibenzolar-S-Methyl for the Control of Bacterial Spot on Processing Tomato

et al. 2016

View full text Add to dashboard Cite

Acibenzolar-S-methyl (ASM) is a plant activator that triggers systemic acquired resistance that is labeled for use in Brazil for managing tomato bacterial spot. The aim of this study was to define the optimum relationship between interval and number of applications of ASM for the most efficacious control of bacterial spot on processing tomato. Four intervals between applications (4, 7, 10, and 14 days) and four application frequencies (4, 6, 8, and 10 applications) were evaluated in five trials performed between 2010 and 2011 in Brasília, Distrito Federal, and in Morrinhos, in the state of Goiás. Copper hydroxide (CH) was applied after ASM applications to complete 13 applications per growing season. Two check treatments were added: standard CH weekly applications and untreated. The following variables were evaluated: severity of bacterial spot, yield, percentage of ripe and rotten fruit, soluble solids content, and the benefit/cost ratio. Disease severity varied between treatments in three trials and yield varied in two trials. A greater number of applications resulted in a reduction of bacterial spot, and the ideal interval between applications was between 8 to 10 days. However, there was a reduction in yield with 10 ASM applications. The temporal effect of ASM applications on bacterial spot severity was evaluated under greenhouse conditions. Reduction in disease severity was observed up to 6 days following ASM application. Additionally, the weekly treatment of seven applications of ASM followed by six applications of CH was compared with a standard program of CH under commercial production conditions in Itaberaí, Goiás, where bacterial spot occurred naturally. The program with ASM and CH resulted in significantly less foliar disease severity than the standard program, which did not result in yield gains. In addition, no differences were detected between the two programs for soluble solids content, industrial yield, plant height, and percentage of ripe and rotten fruit.

show abstract

Establishment of a procedure for bacterial spot inoculation and assessment in processing tomato field trials

Cited by 4 publications

References 14 publications

Magnesium Oxide Nanomaterial, an Alternative for Commercial Copper Bactericides: Field-Scale Tomato Bacterial Spot Disease Management and Total and Bioavailable Metal Accumulation in Soil

Magnesium Oxide Nanomaterial, an Alternative for Commercial Copper Bactericides: Field-Scale Tomato Bacterial Spot Disease Management and Total and Bioavailable Metal Accumulation in Soil

Media pH and media type can significantly affect the reliability of in vitro copper tolerance assessments of Pseudomonas syringae pv. tomato

Intervals and Number of Applications of Acibenzolar-S-Methyl for the Control of Bacterial Spot on Processing Tomato

Contact Info

Product

Resources

About