Establishment of a rapeseed meal fermentation model for iturin A production by <i>Bacillus amyloliquefaciens </i><scp>CX</scp>‐20

Chen, Wenchao; Ma, Xiaoxuan; Wang, Xiuzhen; Chen, Shouwen; Rogiewicz, Anna; Slominski, B. A.; Wan, Xia; Huang, Fenghong

doi:10.1111/1751-7915.13483

Cited by 12 publications

(17 citation statements)

References 44 publications

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“…Iturin A is an important antifungal cyclic lipopeptide with wide application prospects. In previous studies, we verified the advantages of direct bio-utilization of untreated RSM for effective iturin A production by Bacillus in submerged fermentation, which compared favourably with commercial nitrogen sources (Jin et al, 2014(Jin et al, , 2015Chen et al, 2019a). Moreover, the potential industrial strain B. amyloliquefaciens CX-20 was able to achieve high iturin A productivity by directly using RSM for all nutrients except for the carbon source (Chen et al, 2019a).…”

Section: Introductionsupporting

confidence: 63%

“…(2014) and Chen et al . (2019a), which demonstrated that almost no iturin A were produced when 8 g l −1 ammonium nitrate was used as the nitrogen source. However, when an equal amount of ammonium nitrate was added into RSM medium, the yield of iturin A reached 1.07 g l −1 in spite of the negative effects of ammonium nitrate (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, we verified the advantages of direct bio‐utilization of untreated RSM for effective iturin A production by Bacillus in submerged fermentation, which compared favourably with commercial nitrogen sources (Jin et al, 2014, 2015; Chen et al ., 2019a). Moreover, the potential industrial strain B. amyloliquefaciens CX‐20 was able to achieve high iturin A productivity by directly using RSM for all nutrients except for the carbon source (Chen et al ., 2019a). Even though it was not optimal, when glucose was used as the carbon source, the maximum production of iturin A by B. amyloliquefaciens CX‐20 after 72 h of batch fermentation in shake flasks reached up to 1.25 g l −1 .…”

Section: Introductionmentioning

confidence: 99%

“…This value corresponded to a yield of 13.89 g iturin A per kg RSM, which was very close to the highest production level reported to date. This previous record of 14 g iturin A per kg dry substrate was obtained using B. subtilis RB14‐CS in a medium based on soybean curd residue (Mizumoto et al ., 2007; Chen et al ., 2019a). However, whether fungal pretreatment can further improve the synthesis of iturin A is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX‐20

Chen

Wang

Gong

et al. 2020

Microbial Biotechnology

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Fungal pretreatment is the most common strategy for improving the conversion of rapeseed meal (RSM) into value-added microbial products. It was demonstrated that Bacillus amyloliquefaciens CX-20 could directly use RSM as the sole source of all nutrients except the carbon source for iturin A fermentation with high productivity. However, whether fungal pretreatment has an impact on iturin A production is still unknown. In this study, the effects of fungal pretreatment and direct bio-utilization of RSM for iturin A fermentation were comparatively analysed through screening suitable fungal species, and evaluating the relationships between iturin A production and the composition of solid fermented RSM and liquid hydrolysates. Three main unconventional adverse effects were identified. (1) Solid-state fermentation by fungi resulted in a decrease of the total nitrogen for B. amyloliquefaciens CX-20 growth and metabolism, which caused nitrogen waste from RSM. (2) The released free ammonium nitrogen in liquid hydrolysates by fungal pretreatment led to the reduction of iturin A. (3) The insoluble precipitates of hydrolysates, which were mostly ignored and wasted in previous studies, were found to have beneficial effects on producing iturin A. In conclusion, our study verifies the unconventional adverse effects of fungal pretreatment on iturin A production by B. amyloliquefaciens CX-20 compared with direct bioutilization of RSM.

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Section: Introductionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX‐20

Chen

Wang

Gong

et al. 2020

Microbial Biotechnology

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“…Iturin A was extracted according to a reported method [4, 5, 24]. Briefly, 0.3 mL of the mixed fermentation broth was added into a 2 mL glass tube containing 1.2 mL of methanol, shaken well and incubated for 60 min.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

Chen

et al. 2019

BMC Biotechnol

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BackgroundRapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, RSC has been rarely used as a raw material for microbial fermentation, although both RSC and RSM are mainly composed of proteins, carbohydrates and minerals. In this study, we investigated the feasibility of using untreated low-cost RSC as nitrogen source to produce the valuable cyclic lipopeptide antibiotic iturin A using Bacillus amyloliquefaciens CX-20 in submerged fermentation. Especially, the effect of oil in RSC on iturin A production and the possibility of using lipases to improve the iturin A production were analyzed in batch fermentation.ResultsThe maximum production of iturin A was 0.82 g/L at the optimal initial RSC and glucose concentrations of 90 and 60 g/L, respectively. When RSC was substituted with RSM as nitrogen source based on equal protein content, the final concentration of iturin A was improved to 0.95 g/L. The production of iturin A was further increased by the addition of different lipase concentrations from 0.1 to 5 U/mL into the RSC medium for simultaneous hydrolysis and fermentation. At the optimal lipase concentration of 0.5 U/mL, the maximal production of iturin A reached 1.14 g/L, which was 38.15% higher than that without any lipase supplement. Although rapeseed oil and lipase were firstly shown to have negative effects on iturin A production, and the effect would be greater if the concentration of either was increased, their respective negative effects were reduced when used together.ConclusionsAppropriate relative concentrations of lipase and rapeseed oil were demonstrated to support optimal iturin A production. And simultaneous hydrolysis with lipase and fermentation was an effective way to produce iturin A from RSC using B. amyloliquefaciens CX-20.

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Surfactant‐aided mycoremediation of soil contaminated with polycyclic aromatic hydrocarbon (PAHs): progress, limitation, and countermeasures

Rathankumar

Saikia

Kumar

et al. 2021

J of Chemical Tech & Biotech

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Soil contaminated with hydrophobic organic hydrocarbons, such as polycyclic aromatic hydrocarbons (PAHs), pose a serious threat to the ecosystem and human health. Moreover, the sorption and desorption of the PAHs, due to the interaction with the soil organic matter (SOM) and other contaminants, greatly affect the fate and mycoremediation efficiency of the contaminated soil. On the other hand, the application of surfactants with or without additives for the mobilization of PAHs from contaminated soil has garnered increasing attention for soil remediation. In this context, several commercial surfactants and biosurfactants were reported either as soil flushing agents or soil additives, followed by either in situ or ex situ mycoremediation of the PAHs from contaminated soil. Hence, for a successful implementation of surfactants for mycoremediation of contaminated soil, a comprehensive introspection on its chemistry and mechanism is necessary. Thus, this review focuses primarily on understanding the effect of surfactants and the mechanism of desorption of PAHs from the soil. This work also emphasizes the effect of surfactants on the efficiency of mycoremediation of contaminated soil. Further, the review summarizes various surfactant-assisted mycoremediation strategies of contaminated soil. Finally, the limitations of surfactant-assisted mycoremediation, precipitative, and partitioning loss, along with toxicity of the surfactants, were reviewed. Thus, this review will aid in understanding the underlying mechanism and efficiency of surfactant-assisted mycoremediation and it also proposes current research trends to improve the efficiency and enhance the bioremediation of PAHs-contaminated soil.

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Establishment of a rapeseed meal fermentation model for iturin A production by Bacillus amyloliquefaciens CX‐20

Cited by 12 publications

References 44 publications

The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX‐20

The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX‐20

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

Surfactant‐aided mycoremediation of soil contaminated with polycyclic aromatic hydrocarbon (PAHs): progress, limitation, and countermeasures

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