Establishment of a rare minnow (Gobiocypris rarus) disease model for grass carp reovirus genotype II

Chen, Jiaming; Chang, Ouqin; Li, Yingying; Wang, Yingying; Liu, Chao; Yin, Jun; Bergmann, Sven; Zeng, Weiwei; Wang, Qing

doi:10.1016/j.aquaculture.2020.736133

Cited by 7 publications

(11 citation statements)

References 35 publications

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“…Grass carp is the natural host of GCRV, while the sensitivity of the rare minnow is higher. This species also can be used for GCRV as an important model animal to evaluate the GCRV virulence [ 23 , 46 ]. In our study, rare minnow and grass carp infected with GZ1208 did not exhibit hemorrhagic symptoms and resulted in a cumulative mortality of <10%.…”

Section: Discussionmentioning

confidence: 99%

Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Zeng

Bergmann

Dong

et al. 2021

Viruses

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The hemorrhagic disease of grass carp (HDGC) caused by grass carp reovirus (GCRV) still poses a great threat to the grass carp industry. Isolation and identification of the GCRV genotype I (GCRV-I) has been rarely reported in the past decade. In this study, a new GCRV was isolated from diseased fish with severe symptoms of enteritis and mild hemorrhages on the body surface. The isolate was further identified by cell culture, transmission electron, indirect immunofluorescence, and SDS-PAGE electrophoretic pattern analysis of genomic RNA. The results were consistent with the new isolate as a GCRV-I member and tentatively named GCRV-GZ1208. Both grass carp and rare minnow infected by the GCRV-GZ1208 have no obvious hemorrhagic symptoms, and the final mortality rate was ≤10%, indicating that it may be a low virulent isolate. GZ1208 possessed highest genomic homology to 873/GCHV (GCRV-I) and golden shiner reovirus (GSRV). Additionally, it was found a 90.7–98.3% nucleotide identity, a 96.4–100% amino acid identity, and <50% identity with GCRV-II and III genotypes. Interestingly, the sequences of some segments of GZ1208 were similar to GCRV-8733/GCHV, whereas the remaining segments were more closely related to GSRV, suggesting that a recombination event had occurred. Bootscan analysis of the complete genomic sequence confirmed this hypothesis, and recombination events between 873/GCHV and other GSRV-like viruses were also accompanied by gene mutations.

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Section: Discussionmentioning

confidence: 99%

Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Zeng

Bergmann

Dong

et al. 2021

Viruses

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“…Genotype II GCRV differentiates from genotype I and III GCRVs in lacking suitable in vitro systems to sustain stable viral propagation and study virus cytopathogenicity (Chen et al, 2021). Although grass carp represents the most productive breed in freshwater F I G U R E 2 Determination of absolute lethal dose (LD 100 ) and semi-lethaldose (LD 50 ) of GCRV-JX02 in rare minnow.…”

Section: Discussionmentioning

confidence: 99%

“…GCRV has been heavily studied in recent years due to its pathogenic nature and negative impact on grass carp ( Ctenopharyngodon idellus ) production (Yu et al, 2018). Genotype II GCRV differentiates from genotype I and III GCRVs in lacking suitable in vitro systems to sustain stable viral propagation and study virus cytopathogenicity (Chen et al, 2021). Although grass carp represents the most productive breed in freshwater aquaculture, no experimental SPF grass carp species with clear genetic background is available for researchers.…”

Section: Discussionmentioning

confidence: 99%

“…Rare minnow ( Gobiocypris rarus ) is an eurythermal small cyprinid fish distributed in the natural water of southwest of China, which emerges as a superior laboratory fish with broad application prospects in toxicology (Mu et al, 2020), genetics and biomedicine (Ma et al, 2007). Interestingly, this model fish with clear genetic background is sensitive to GCRV infection and provides a sensitive bioassay to assess GCRV infection and anti‐GCRV immunology (Chen et al, 2021). Artificial infection of the rare minnow with genotype II GCRV results in the rapid onset of haemorrhagic symptoms and near 100% mortality (Lin et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Quercetin protects rare minnow Gobiocypris rarus from infection of genotype II grass carp reovirus

Sun

et al. 2021

Aquaculture Research

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Plant-derived quercetin is a major dietary flavonoid and known mammalian transcriptional repressor for heat shock factor 1 (HSF-1), which is responsible for the transcriptional activation of heat shock proteins upon stress stimulation. Our previous studies indicated that Hsp70, the major heat shock protein during heat shock response, is induced to facilitate the infection of grass carp reovirus (GCRV), and quercetin could reduce the replication efficiency of both genotype I and III GCRVs in vitro. Here, we provide in vivo evidence to show that quercetin could effectively increase the survival rate of rare minnow Gobiocypris rarus from genotype II GCRV (GCRV-JX02) infection.Quercetin is shown to be safe for rare minnow in a dosage range of 30-480 μg/fish.Infection of rare minnow with GCRV-JX02 results in typical haemorrhagic symptom and tissue damage in pathological examination, and the semi-lethal dose (LD 50 ) of GCRV-JX-02 is determined to be 6.0 × 10 2 virus genome copy number through a quantitative real-time RT-PCR assay. Quercetin dose-dependently protects rare minnow from GCRV-JX02 challenge through decreasing progeny virus production and increasing fish survival rate. Thus, quercetin demonstrates anti-aquareovirus activity in vivo and should act as a promising environmentally friendly therapeutic agent for aquaculture.

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“…GCHD is not confined to grass carp, and the rare minnow ( Gobiocypris rarus ) is as sensitive to GCRV II as grass carp ( 20 ). In addition, the use of the rare minnow as the model for evaluation of experimental vaccines against GCRV II infections has many advantages such as its small size, facile care, transparent egg membrane and annual spawning.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Lactococcus lactis Expressing Grass Carp Reovirus VP6 Induces Mucosal Immunity Against Grass Carp Reovirus Infection

Wang

et al. 2022

Front. Immunol.

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Grass carp haemorrhagic disease caused by grass carp reovirus II is a serious disease of the aquaculture industry and vaccination is the only effective method of GCRV protection. In this study, Lactococcus lactis was used as oral vaccine delivery to express the GCRV II VP6 protein. We evaluated the protective efficacy of the live vaccine strain to induce mucosal immune protection. After oral administration, the recombinant strains remained in the hindgut for antigen presentation and increased the survival rate 46.7% and the relative percent survival 42.9%, respectively versus control vaccination. Though L. lactis alone can induce the inflammatory response by stimulating the mucosal immune system, the recombinant L. lactis expressing VP6 greatly enhanced nonspecific immune responses via expression of immune related genes of the fish. Furthermore, both systemic and mucosal immunity was elicited following oral immunization with the recombinant strain and this strain also elicited an inflammatory response and cellular immunity to enhance the protective effect. L. lactis can therefore be utilized as a mucosal immune vector to trigger high levels of immune protection in fish at both the systemic and mucosal levels. L. lactis is a promising candidate for oral vaccine delivery.

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Establishment of a rare minnow (Gobiocypris rarus) disease model for grass carp reovirus genotype II

Cited by 7 publications

References 35 publications

Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Quercetin protects rare minnow Gobiocypris rarus from infection of genotype II grass carp reovirus

Recombinant Lactococcus lactis Expressing Grass Carp Reovirus VP6 Induces Mucosal Immunity Against Grass Carp Reovirus Infection

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