Establishment of a Rat Model for Alveolar Cleft With Bone Wax

Xu, Yue; Sun, Jian; Chen, Zhenqi

doi:10.1016/j.joms.2014.12.010

Cited by 12 publications

(7 citation statements)

References 17 publications

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“…Several models have been proposed as alveolar cleft model for testing of tissue-engineered bone replacement material. These ranging from mice, rats, rabbits, cats, dogs, swines, goats, sheep and monkeys [ 4 , 9 – 24 ]. Prior description on rats models were able to create defects simulating alveolar defects because of their ease of handling and cost effectiveness, however, these defects tend to be significantly smaller in volume than human alveolar defects making it technically challenging to properly perform the grafting testing procedure [ 13 , 17 , 18 , 21 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Most current reports in the literature illustrated the creation of simple non-anatomical defects in the maxilla of small animals by creating a maxillary or palatal window to establish a communication between the oral cavity and the nasal cavity, as described by Nguyen et al, Raposo-Amaral et al, Mostafa et al, Takano-Yamamoto et al and Kim et al in rats, and by Sawada et al and Puumanen et al in rabbits [ 17 , 18 , 21 , 22 , 39 – 41 ]. Xu et al described the establishment of a cleft model in rats by extracting a molar tooth and applying bone wax [ 24 ]. Their model has succeeded in controlling the osseous healing process but the anatomical location of the defect does not correspond to a tridimensional maxillary alveolar cleft defects as encountered clinically.…”

Section: Discussionmentioning

confidence: 99%

“…Animal models with simulated alveolar clefts are considered appropriate as an experimental model for testing of clinical interventions. Several animal models have been utilized for testing of alveolar cleft grafting materials including mice, rats, rabbits, cats, dogs, swines, goats, sheep and monkeys [ 4 , 9 – 24 ].…”

Section: Introductionmentioning

confidence: 99%

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A rabbit model for experimental alveolar cleft grafting

et al. 2017

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ObjectivesThe purpose of the present study was to develop an animal model for creating alveolar cleft defects with properly simulated clinical defect environment for tissue-engineered bone-substitute materials testing without compromising the health of the animal. Cleft creation surgery was aimed at creating a complete alveolar cleft with a wide bone defect with an epithelial lining (oral mucosa) overlying the cleft defect.MethodsA postmortem skull of a New Zealand White (NZW) rabbit skull (Oryctolagus cuniculus) underwent an osteological and imaging survey. A pilot postmortem surgery was conducted to confirm the feasability of a surgical procedure and the defect was also radiologically confirmed and illustrated with micro-computed tomography. Then, a surgical in vivo model was tested and evaluated in 16 (n = 16) 8-week-old NZW rabbits to create in vivo alveolar cleft creation surgery.ResultsClinical examination and imaging analysis 8 weeks after cleft creation surgery revealed the establishment of a wide skeletal defect extending to the nasal mucosa simulating alveolar clefts in all of the rabbits.ConclusionsOur surgical technique was successful in creating a sizable and predictable model for bone grafting material testing. The model allows for simulating the cleft site environment and can be used to evaluate various bone grafting materials in regard to efficacy of osteogenesis and healing potential without compromising the health of the animal.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A rabbit model for experimental alveolar cleft grafting

et al. 2017

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“…En relación a la fisura alveolar, existen numerosos estudios en animales donde se realiza una fisura alveolar quirúrgica en monos (Boyne et al, 1998), perros (Mayer et al, 1996;Kawamoto et al, 2002), conejos (el-Bokle et al, 1993) o ratas (Nguyen et al, 2009;Nampo et al, 2010;Mostafa et al, 2014;Xu et al, 2015), en donde se crea un defecto óseo crítico para el estudio de los injertos de la fisura alveolar en humanos. Se han comparado rhBMP-2 con distintos tipos de injertos o también se han estudiado distintas matrices para llevar la rhBMP-2 al defecto óseo (Tabla I).…”

Section: Estructura Molecular Y Actividad Biológicaunclassified

Proteína Morfogenética Ósea y su Opción como Tratamiento de la Fisura Alveolar

et al. 2017

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1,10URIBE, F.; CANTÍN, M.; ALISTER, J.P.; VILOS, C.; FARIÑA, R. & OLATE, S. Proteína morfogenética ósea y su opción como tratamiento de la fisura alveolar. Int. J. Morphol., 35(1):310-318, 2017.RESUMEN:La proteína morfogenética ósea (BMP), es una proteína endógena que ha mostrado efectos significativos en la promoción de la formación ósea. El uso de BMP ha sido descrito en la reconstrucción de defectos óseos de origen traumáticos y patológicos, incluyendo la fisura alveolar, el aumento de reborde alveolar, la elevación de seno maxilar, el injerto de alveolo post-extracción, y la cirugía perimplantaria entre otros. A pesar de las ventajas asociadas al uso de BMP y que en la actualidad se aplica en combinación con matrices de colágeno, ciertas propiedades tales como su baja resistencia mecánica y su elevada tasa de liberación inicial disminuyen su eficacia en la formación ósea. En este contexto, el desarrollo de nuevos sistemas de liberación prolongada de BMP que permitan la quimiotaxis de células mesenquimáticas y su posterior diferenciación a osteoblastos representa un desafío con alto potencial clínico para la estimulación de la formación ósea. En este trabajo, se describe el uso de BMP en la reconstrucción de fisuras alveolares y en particular se discuten las ventajas de su administración en micropartículas poliméricas como sistemas de liberación de BMP (rhBMP-2) con promisorias aplicaciones en la estimulación de la formación ósea.PALABRAS CLAVE: Proteína morfogenética ósea; rhBMP-2; Fisura alveolar; Micropartículas. INTRODUCCIÓNLas proteínas morfogenéticas óseas (BMPs) son un conjunto de proteínas endógenas que pertenecen a la familia de factor de crecimiento transformante beta (TGF-β) y que tienen la capacidad de inducir formación del hueso, cartílago y el tejido conectivo (Carreira et al., 2014a).Las BMPs fueron descritas por el Dr. Marshal Urist en 1965(Urist, 1965, al implantar matriz ósea desmineralizada extraída de bovino a nivel intramuscular en ratas y conejos y observar la producción ósea ectópica en el sitio de implantación; él lo denominó formación ósea por autoinducción. Urist atribuyó el proceso a la presencia de una proteína que atrae células mesenquimáticas pluripotenciales e induce formación ósea local, por lo que la denominó proteína morfogenética ósea (BMP). Actualmente, se han identificado 20 tipos distintos de BMP, sin embargo sólo la BMP-2, BMP-7 y BMP-9 son capaces de promover la osteoinducción. Pese a que BMP es una proteína endógena que puede ser obtenida de tejido óseo desmineralizado, su aislamiento es inviable debido a que se encuentra en mínimas cantidades (Wozney et al., 1988). Estudios descritos en bovinos mostraron que es posible obtener 57 mg de un extracto rico en BMPs por kilo de hueso fresco. Sin embargo, luego de realizar los procesos de purificación y aislamiento de una BMP en específi-co, el rendimiento se redujo a unos cuantos ng de BMP por kg de hueso (Carreira et al., 2014a).Por otra parte, basados en las posibles reacciones inmunológicas que pueden ocasionar la utili...

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“…Despite recent biotechnological developments of bone substitute materials, optimising the quality of the existing regenerative materials and looking for novel and more effective materials is essential in developing a clinically suitable material. The establishment of a proper in vivo biological model simulating alveolar clefts is essential in order to conduct experimental testing of bone grafting materials and evaluate the clinical effect with respect to osteogenesis and healing, and thus far several animal models have been developed in mice, rats, rabbits, cats, dogs, swines, goats, sheep and monkeys [ 2 , 6 , 35 – 49 ].…”

Section: Introductionmentioning

confidence: 99%

Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model

et al. 2017

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BackgroundAlveolar cleft repair is performed via bone grafting procedure to restore the dental arch continuity. A suitable bone substitute materials should possess osteoinductive and osteoconductive properties, to promote new bone formation, along with a slowly resorbable scaffold that is subsequently replaced with functionally viable bone. Calcium phosphate biomaterials have long proved their efficacy as bone replacement materials. Dentin in several forms has also demonstrated its possibility to be used as bone graft replacement material in several studies. The purpose of this study was to evaluate bone regeneration pattern and quantify bone formation after grafting pre-established experimental alveolar clefts defects model in rabbits using composite xenogenic dentin and β-TCP in comparison to β-TCP alone.MethodsUnilateral alveolar cleft defects were created in 16 New Zealand rabbits according to previously described methodology. Alveolar clefts were allowed 8 weeks healing period. 8 defects were filled with β-TCP, whereas 8 defects filled with composite xenogenic dentin with β-TCP. Bone regeneration of the healed defects was compared at the 8 weeks after intervention. Quantification of bone formation was analyzed using micro-computed tomography (µCT) and histomorphometric analysis.ResultsµCT and histomorphometric analysis revealed that defects filled with composite dentin/β-TCP showed statistically higher bone volume fraction, bone mineral density and percentage residual graft volume when compared to β-TCP alone. An improved surgical handling of the composite dentin/β-TCP graft was also noted.ConclusionsComposite xenogenic dentin/β-TCP putty expresses enhanced bone regeneration compared to β-TCP alone in the reconstruction of rabbit alveolar clefts defects.

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Establishment of a Rat Model for Alveolar Cleft With Bone Wax

Cited by 12 publications

References 17 publications

A rabbit model for experimental alveolar cleft grafting

A rabbit model for experimental alveolar cleft grafting

Proteína Morfogenética Ósea y su Opción como Tratamiento de la Fisura Alveolar

Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model

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