Establishment of a rat model with ageing insomnia induced by D‑galactosef and para‑chlorophenylalanine

Ren, Xiaojuan; Wang, Qingquan; Zhang, Xingping; Wang, Guan‐Ying; Liu, Tao; Deng, Ning; Yan, Di

doi:10.3892/etm.2020.9080

Cited by 7 publications

(6 citation statements)

References 61 publications

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“…Similarly, IL-6 levels were increased in the rat brain. 57,59–61 Also, molecular docking study revealed high affinity of rutin to IL6RA and IL6RB indicating the ability of rutin to reduce aging-associated inflammatory process.…”

Section: Discussionmentioning

confidence: 99%

Rutin attenuates d-galactose-induced oxidative stress in rats’ brain and liver: molecular docking and experimental approaches

et al. 2023

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Section: Discussionmentioning

confidence: 99%

Rutin attenuates d-galactose-induced oxidative stress in rats’ brain and liver: molecular docking and experimental approaches

et al. 2023

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“…To investigate the potential mechanisms linking insomnia and cognitive impairment, we first established a PCPA-induced insomniac model. Compared with other models, , such as the horizontal platform environmental deprivation method, the stress stimulation sleep deprivation method, the gentle stimulation deprivation method, and the forced exercise deprivation method, our model has a short modeling cycle, clear target, and significant insomnia characteristics and is suitable for acute insomnia studies . Currently, a variety of behavioral tests, including the Morris water maze test, Y-maze test, novel place recognition test, and novel object recognition (NOR) test, have been used to investigate the cognitive ability.…”

Section: Discussionmentioning

confidence: 99%

The Combination of Quantitative Proteomics and Systems Genetics Analysis Reveals that PTN Is Associated with Sleep-Loss-Induced Cognitive Impairment

Zhou,

Li,

Liu

et al. 2023

J. Proteome Res.

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Sleep loss is associated with cognitive dysfunction. However, the detailed mechanisms remain unclear. In this study, we established a parachlorophenylalanine (PCPA)-induced insomniac mouse model with impaired cognitive function. Mass-spectrometry-based proteomics showed that the expression of 164 proteins was significantly altered in the hippocampus of the PCPA mice. To identify critical regulators among the potential markers, a transcriptome-wide association screening was performed in the BXD mice panel. Among the candidates, the expression of pleiotrophin (Ptn) was significantly associated with cognitive functions, indicating that Ptn-mediates sleep-loss-induced cognitive impairment. Gene co-expression analysis further revealed the potential mechanism by which Ptn mediates insomnia-induced cognitive impairment via the MAPK signaling pathway; that is, the decreased secretion of Ptn induced by insomnia leads to reduced binding to Ptprz1 on the postsynaptic membrane with the activation of the MAPK pathway via Fos and Nr4a1, further leading to the apoptosis of neurons. In addition, Ptn is genetically trans-regulated in the mouse hippocampus and implicated in neurodegenerative diseases in human genome-wide association studies. Our study provides a novel biomarker for insomnia-induced cognitive impairment and a new strategy for seeking neurological biomarkers by the integration of proteomics and systems genetics.

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“…Therefore, NREM can be retained to a certain degree by the rats adapting flexible postures, rather than the rats only being allowed to sit on the platforms. Secondly, unlike the deep-water design used for multi-platform devices in previous studies ( 34 ), the shallow water allows the rats to walk freely and to communicate or play with each other without getting most of their body wet, which can protect them from developing depression or soaking-associated skin diseases. Thirdly, although the platforms allow the rats to sleep lightly, they may rotate around the steel wire when the balance between the two sides of the platform is distorted during REM sleep.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a chronic insomnia rat model of sleep fragmentation using unstable platforms surrounded by water

Yan

Zhang

et al. 2023

Exp Ther Med

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Chronic fragmented sleep is a very common type of insomnia that affects the daily lives of numerous people around the world. However, its pathogenesis is not very clear and a corresponding rat model has not been reported for this purpose at present. The present study aimed to establish a rat model of chronic insomnia with sleep fragmentation using self-made multiple strings of unstable platforms surrounded by shallow water. During the establishment of the models, changes in body weight and differences in food and water intake in the daytime and at night were acquired. The rat models were assessed using several tests, including the Morris water maze test, pentobarbital sodium-induced sleep, infrared monitoring and electroencephalogram/electromyography during sleep. The expression levels of certain inflammatory factors and orexin A were detected in the serum and brain tissues using ELISAs, immunohistochemistry and immunofluorescence. The expression levels of orexin 1 receptor (orexin 1r) were also detected in the brain. Polysomnography indicated that the model rats were successfully prepared with reduced non-rapid eye movement (non-REM) sleep in the daytime, which was increased at night, and considerably lower REM duration during the day and night. The number of instances of sleep arousals were also increased in the day and at night, and the average duration of each sleep bout was decreased in the daytime. The body weights of the model rats increased at a normal rate. However, the reduction of body weight in the daytime and increased in body weight at night were significantly less than those of the control rats. The daytime food and water consumption of the model rats increased significantly compared with that of the control rats, but was similar to that of the control group at night. The Morris water maze test indicated that the model rats were slow to learn to escape the platforms and performed a lower number of target crossings. The pentobarbital-induced sleep experiment confirmed that the model rats exhibited a longer sleep latency and shorter sleep time. The serum IL-1β, IL-6, TNF-α and orexin A levels of the model rats were significantly increased, whereas their serum IL-10 levels were significantly decreased compared with those of the control rats. The expression levels of IL-1β, IL-6, orexin A and orexin 1r in the brain tissues of the model rats were also significantly increased. In conclusion, these data indicate that learning and memory function, sleep time, arousal times, diurnal and nocturnal body weight changes, food and water intake, and expression levels of the specific inflammatory factors orexin A and orexin 1r were altered in the model rats. This suggests the chronic insomnia rat model with sleep fragmentation was successfully established using multiple strings of unstable platforms surrounded by water.

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Establishment of a rat model with ageing insomnia induced by D‑galactosef and para‑chlorophenylalanine

Cited by 7 publications

References 61 publications

Rutin attenuates d-galactose-induced oxidative stress in rats’ brain and liver: molecular docking and experimental approaches

Rutin attenuates d-galactose-induced oxidative stress in rats’ brain and liver: molecular docking and experimental approaches

The Combination of Quantitative Proteomics and Systems Genetics Analysis Reveals that PTN Is Associated with Sleep-Loss-Induced Cognitive Impairment

Establishment of a chronic insomnia rat model of sleep fragmentation using unstable platforms surrounded by water

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