2024
DOI: 10.1111/jfs.13119
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Establishment of a real‐time fluorescence and visual colorimetric detection method for Staphylococcus aureus based on loop‐mediated isothermal amplification

Xinping Cui,
Jianping Guo,
Zuwei Wang
et al.

Abstract: A loop‐mediated isothermal amplification (LAMP) method combined with calcein was established for the detection of Staphylococcus aureus. LAMP primers were designed targeting the ASL18_04625 species‐specific gene screened through bioinformatics and PCR analysis. The target gene and detection method demonstrated 100% specificity for S. aureus and no cross‐reactions with other pathogens. For LAMP reactions, the results could be directly observed with the naked eye or evaluated by S‐shaped amplification curves of … Show more

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